Antiseizure medications and fetal nutrients: Effects on choline transporters in a human placental cell line

Epilepsia ◽  
2021 ◽  
Author(s):  
Nino Tetro ◽  
Sonia Moushaev ◽  
Miri Shmuel ◽  
Sara Eyal
Keyword(s):  
Cell Line ◽  
Placental Cell

scholarly journals Selective Impairment of Taurine Transport by Cyclosporin A in a Human Placental Cell Line

1992 ◽  
Vol 32 (1) ◽  
pp. 125-127 ◽  
Author(s):  
Sammanda Ramamoorthy ◽  
Frederick H Leibach ◽  
Virendra B Mahesh ◽  
Vadivel Ganapathy
Keyword(s):  
Cyclosporin A ◽  
Cell Line ◽  
Taurine Transport ◽  
Placental Cell ◽  
Selective Impairment

scholarly journals Regulation of glutamate transport and transport proteins in a placental cell line

2001 ◽  
Vol 281 (3) ◽  
pp. C1014-C1022 ◽  
Author(s):  
Donald Novak ◽  
Forrest Quiggle ◽  
Carlos Artime ◽  
Mark Beveridge
Keyword(s):  
Amino Acid ◽  
Cell Line ◽  
Glutamate Uptake ◽  
Membrane Vesicle ◽  
Transport Proteins ◽  
Cell Surface Expression ◽  
Acid Uptake ◽  
Placental Cell ◽  
Amino Acid Availability ◽  
Amino Acid Depletion

We utilized HRP.1 cells derived from midgestation rat placental labyrinth to determine that the primary pathway for glutamate uptake is via system X[Formula: see text], a Na+-dependent transport system. Kinetic parameters of system X[Formula: see text]activity were similar to those previously determined in rat and human placental membrane vesicle preparations. Amino acid depletion caused a significant upregulation of system X[Formula: see text] activity at 6, 24, and 48 h. This increase was reversed by the addition of glutamate and aspartate but not by the addition of α-(methylamino)isobutyric acid. Immunoblot analysis of the three transport proteins previously associated with system X[Formula: see text] activity indicated a trend toward an increase in GLT1, EAAC1, and GLAST1 immunoreactive protein contents by 48 h; cell surface expression of the same was enhanced by 24 h. Inhibition analysis suggested key roles for EAAC1 and GLAST1 in basal anionic amino acid transfer, with an enhanced role for GLT1 under conditions of amino acid depletion. In summary, amino acid availability as well as intracellular metabolism regulate anionic amino acid uptake into this placental cell line.

Establishment of a rat placental cell line expressing characteristics of extraembryonic membranes

1987 ◽  
Vol 124 (1) ◽  
pp. 134-144 ◽  
Author(s):  
Michael J. Soares ◽  
Kathleen D. Schaberg ◽  
Catherine S. Pinal ◽  
Swapan K. De ◽  
Paramjit Bhatia ◽  
...  
Keyword(s):  
Cell Line ◽  
Placental Cell ◽  
Extraembryonic Membranes

The Effects of Fungicides on Human 3β-Hydroxysteroid Dehydrogenase 1 and Aromatase in Human Placental Cell Line JEG-3

Pharmacology ◽  
2017 ◽  
Vol 100 (3-4) ◽  
pp. 139-147 ◽  
Author(s):  
Shuyan Cao ◽  
Leping Ye ◽  
Ying Wu ◽  
Baiping Mao ◽  
Lanlan Chen ◽  
...  
Keyword(s):  
Cell Line ◽  
Inhibitory Concentration ◽  
Fungal Infections ◽  
Hydroxysteroid Dehydrogenase ◽  
Competitive Inhibitor ◽  
Steroid Production ◽  
Placental Cell ◽  
Ic50 Values ◽  
Agricultural Plants ◽  
Noncompetitive Inhibitors

Placenta secretes a large amount of progesterone and estradiol, which are critical for maintaining pregnancy. In human placenta, 3β-hydroxysteroid dehydrogenase 1 (HSD3B1) catalyzes pregnenolone to form progesterone, and aromatase (CYP19A1) catalyzes testosterone into estradiol. Fungicides display antifungal activities and are widely used to prevent fungal infections in agricultural plants. These chemicals include azoles, such as tebuconazole (TEB), triadimefon (TRI), and vinclozolin (VCZ) or organotins, such as tributyltin (TBT) and tetrabutyltin (TTBT). Fungicides may disrupt the activities of these 2 enzymes. In the present study, we investigated the effects of these fungicides on steroid production in a human placental cell line JEG-3 and on HSD3B1 and CYP19A1 activities. Of all fungicides tested at 100 µmol/L, only TBT inhibited pregnenolone-mediated progesterone production in JEG-3 cells by over 50%. Except TTBT, all other 4 fungicides inhibited testosterone-mediated estradiol production by over 50%. TBT was a moderate HSD3B1 inhibitor with a half maximal inhibitory concentration (IC50) of 45.60 ± 0.12 µmol/L. When pregnenolone was used to determine the mode of inhibition, TBT was a competitive inhibitor of HSD3B1. The IC50 values of TEB, TRI, VCZ, and TBT for CYP19A1 were 56.84 ± 0.13, 58.73 ± 0.14, 57.42 ± 0.171, and 4.58 ± 0.048 µmol/L, respectively. TEB, TRI, and VCZ were noncompetitive inhibitors of CYP19A1, while TBT was a competitive inhibitor of this enzyme. Therefore, they are endocrine disruptors.

scholarly journals Characterization of VAR2CSA-deficient Plasmodium falciparum-infected erythrocytes selected for adhesion to the BeWo placental cell line

2008 ◽  
Vol 7 (1) ◽  
pp. 51 ◽  
Author(s):  
Francisca Yosaatmadja ◽  
Katherine T Andrews ◽  
Michael F Duffy ◽  
Graham V Brown ◽  
James G Beeson ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Cell Line ◽  
Placental Cell

scholarly journals Tert-Butyl Hydroperoxide Stimulated Apoptosis Independent of Prostaglandin E2 and IL-6 in the HTR-8/SVneo Human Placental Cell Line

2020 ◽  
Vol 27 (11) ◽  
pp. 2104-2114 ◽  
Author(s):  
Rita Loch-Caruso ◽  
Cassandra S. Korte ◽  
Kelly A. Hogan ◽  
Sarah Liao ◽  
Craig Harris
Keyword(s):  
Prostaglandin E2 ◽  
Cell Line ◽  
Butyl Hydroperoxide ◽  
Placental Cell ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide

Effect of copper on the cytotoxicity of phenanthrene and 9,10-phenanthrenequinone to the human placental cell line, JEG-3

2007 ◽  
Vol 23 (4) ◽  
pp. 513-520 ◽  
Author(s):  
Z PETERS ◽  
J NYKAMP ◽  
K PASSAPERUMA ◽  
J CARLSON ◽  
S DEWITTEORR ◽  
...  
Keyword(s):  
Cell Line ◽  
Placental Cell ◽  
Effect Of Copper
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