scholarly journals Curcumin reduces development of seizurelike events in the hippocampal‐entorhinal cortex slice culture model for epileptogenesis

Epilepsia ◽  
2019 ◽  
Vol 60 (4) ◽  
pp. 605-614 ◽  
Author(s):  
Cato M. Drion ◽  
Lieneke Kooijman ◽  
Eleonora Aronica ◽  
Erwin A. Vliet ◽  
Wytse J. Wadman ◽  
...  
Keyword(s):  
Entorhinal Cortex ◽  
Slice Culture ◽  
Cortex Slice ◽  
Culture Model

scholarly journals Preparation of organotypic brain slice cultures for the study of Alzheimer’s disease

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 592
Author(s):  
Cara L. Croft ◽  
Wendy Noble
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Brain Slice ◽  
Synaptic Dysfunction ◽  
Slice Culture ◽  
Therapeutic Effects ◽  
Culture Model ◽  
Organotypic Brain Slice ◽  
Brain Slice Cultures

Alzheimer's disease, the most common cause of dementia, is a progressive neurodegenerative disorder characterised by amyloid-beta deposits in extracellular plaques, intracellular neurofibrillary tangles of aggregated tau, synaptic dysfunction and neuronal death. There are no cures for AD and current medications only alleviate some disease symptoms. Transgenic rodent models to study Alzheimer’s mimic features of human disease such as age-dependent accumulation of abnormal beta-amyloid and tau, synaptic dysfunction, cognitive deficits and neurodegeneration. These models have proven vital for improving our understanding of the molecular mechanisms underlying AD and for identifying promising therapeutic approaches. However, modelling neurodegenerative disease in animals commonly involves aging animals until they develop harmful phenotypes, often coupled with invasive procedures. In vivo studies are also resource, labour, time and cost intensive. We have developed a novel organotypic brain slice culture model to study Alzheimer’ disease which brings the potential of substantially reducing the number of rodents used in dementia research from an estimated 20,000 per year. We obtain 36 brain slices from each mouse pup, considerably reducing the numbers of animals required to investigate multiple stages of disease. This tractable model also allows the opportunity to modulate multiple pathways in tissues from a single animal. We believe that this model will most benefit dementia researchers in the academic and drug discovery sectors. We validated the slice culture model against aged mice, showing that the molecular phenotype closely mimics that displayed in vivo, albeit in an accelerated timescale. We showed beneficial outcomes following treatment of slices with agents previously shown to have therapeutic effects in vivo, and we also identified new mechanisms of action of other compounds. Thus, organotypic brain slice cultures from transgenic mouse models expressing Alzheimer’s disease-related genes may provide a valid and sensitive replacement for in vivo studies that do not involve behavioural analysis.

scholarly journals An Ex Vivo Brain Slice Culture Model of Chronic Wasting Disease: Implications for Disease Pathogenesis and Therapeutic Development

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Naveen Kondru ◽  
Sireesha Manne ◽  
Robyn Kokemuller ◽  
Justin Greenlee ◽  
M. Heather West Greenlee ◽  
...  
Keyword(s):  
Brain Slice ◽  
Chronic Wasting Disease ◽  
Ex Vivo ◽  
Slice Culture ◽  
Wasting Disease ◽  
Disease Pathogenesis ◽  
Culture Model ◽  
Therapeutic Development ◽  
Brain Slice Culture

scholarly journals A Myocardial Slice Culture Model Reveals Alpha-1A-Adrenergic Receptor Signaling in the Human Heart

2016 ◽  
Vol 1 (3) ◽  
pp. 155-167 ◽  
Author(s):  
R. Croft Thomas ◽  
Abhishek Singh ◽  
Patrick M. Cowley ◽  
Bat-Erdene Myagmar ◽  
Megan D. Montgomery ◽  
...  
Keyword(s):  
Adrenergic Receptor ◽  
Human Heart ◽  
Slice Culture ◽  
Receptor Signaling ◽  
Culture Model

A brain slice culture model for studies of endogenous and exogenous precursor cell migration in the rostral migratory stream

2009 ◽  
Vol 1295 ◽  
pp. 1-12 ◽  
Author(s):  
Mette Tanvig ◽  
Morten Blaabjerg ◽  
Rikke K. Andersen ◽  
Ana Villa ◽  
Ann Mari Rosager ◽  
...  
Keyword(s):  
Cell Migration ◽  
Brain Slice ◽  
Rostral Migratory Stream ◽  
Precursor Cell ◽  
Slice Culture ◽  
Culture Model ◽  
Brain Slice Culture ◽  
Migratory Stream
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