Effect of a single and repeated stress exposure on gene expression of catecholamine biosynthetic enzymes in brainstem catecholaminergic cell groups in rats

2015 ◽  
Vol 42 (2) ◽  
pp. 1872-1886 ◽  
Author(s):  
Boris Mravec ◽  
Peter Vargovic ◽  
Peter Filipcik ◽  
Michal Novak ◽  
Richard Kvetnansky
Keyword(s):  
Gene Expression ◽  
Biosynthetic Enzymes ◽  
Stress Exposure ◽  
Repeated Stress ◽  
Cell Groups ◽  
Catecholaminergic Cell

scholarly journals Repeated stress exposure results in a survival–reproduction trade-off in Drosophila melanogaster

2009 ◽  
Vol 277 (1683) ◽  
pp. 963-969 ◽  
Author(s):  
Katie E. Marshall ◽  
Brent J. Sinclair
Keyword(s):  
Drosophila Melanogaster ◽  
Model Organism ◽  
Intrinsic Rate ◽  
Intrinsic Rate Of Increase ◽  
Stress Exposure ◽  
Trade Off ◽  
Repeated Stress ◽  
Rate Of Increase ◽  
In The Wild ◽  
Future Reproduction

While insect cold tolerance has been well studied, the vast majority of work has focused on the effects of a single cold exposure. However, many abiotic environmental stresses, including temperature, fluctuate within an organism's lifespan. Given that organisms may trade-off survival at the cost of future reproduction, we investigated the effects of multiple cold exposures on survival and fertility in the model organism Drosophila melanogaster . We found that multiple cold exposures significantly decreased mortality compared with the same length of exposure in a single sustained bout, but significantly decreased fecundity (as measured by r , the intrinsic rate of increase) as well, owing to a shift in sex ratio. This change was reflected in a long-term decrease in glycogen stores in multiply exposed flies, while a brief effect on triglyceride stores was observed, suggesting flies are reallocating energy stores. Given that many environments are not static, this trade-off indicates that investigating the effects of repeated stress exposure is important for understanding and predicting physiological responses in the wild.

Gene expression of catecholamine biosynthetic enzymes following exercise: modulation by age

Neuroscience ◽  
2001 ◽  
Vol 103 (3) ◽  
pp. 703-711 ◽  
Author(s):  
N. Tümer ◽  
H.A. Demirel ◽  
L. Serova ◽  
E.L. Sabban ◽  
C.S. Broxson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Biosynthetic Enzymes

Regulation of Adrenoceptor and Muscarinic Receptor Gene Expression after Single and Repeated Stress

2008 ◽  
Vol 1148 (1) ◽  
pp. 367-376 ◽  
Author(s):  
Jaromir Myslivecek ◽  
Andrej Tillinger ◽  
Martina Novakova ◽  
Richard Kvet��ansk��
Keyword(s):  
Gene Expression ◽  
Muscarinic Receptor ◽  
Receptor Gene ◽  
Repeated Stress ◽  
Receptor Gene Expression

scholarly journals Purity and Enrichment of Laser-Microdissected Midbrain Dopamine Neurons

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Amanda L. Brown ◽  
Trevor A. Day ◽  
Christopher V. Dayas ◽  
Doug W. Smith
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Dopamine Neuron ◽  
Dopamine Neurons ◽  
Acid Decarboxylase ◽  
Cell Groups ◽  
Midbrain Dopamine ◽  
High Degree ◽  
Midbrain Dopamine Neurons

The ability to microdissect individual cells from the nervous system has enormous potential, as it can allow for the study of gene expression in phenotypically identified cells. However, if the resultant gene expression profiles are to be accurately ascribed, it is necessary to determine the extent of contamination by nontarget cells in the microdissected sample. Here, we show that midbrain dopamine neurons can be laser-microdissected to a high degree of enrichment and purity. The average enrichment for tyrosine hydroxylase (TH) gene expression in the microdissected sample relative to midbrain sections was approximately 200-fold. For the dopamine transporter (DAT) and the vesicular monoamine transporter type 2 (Vmat2), average enrichments were approximately 100- and 60-fold, respectively. Glutamic acid decarboxylase (Gad65) expression, a marker for GABAergic neurons, was several hundredfold lower than dopamine neuron-specific genes. Glial cell and glutamatergic neuron gene expression were not detected in microdissected samples. Additionally, SN and VTA dopamine neurons had significantly different expression levels of dopamine neuron-specific genes, which likely reflects functional differences between the two cell groups. This study demonstrates that it is possible to laser-microdissect dopamine neurons to a high degree of cell purity. Therefore gene expression profiles can be precisely attributed to the targeted microdissected cells.

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