Membrane cholesterol regulates different modes of synaptic vesicle release and retrieval at the frog neuromuscular junction

2013 ◽  
pp. n/a-n/a ◽  
Author(s):  
Hermann A. Rodrigues ◽  
Ricardo F. Lima ◽  
Matheus de C. Fonseca ◽  
Ernani A. Amaral ◽  
Patrícia M. Martinelli ◽  
...  
Keyword(s):  
Neuromuscular Junction ◽  
Synaptic Vesicle ◽  
Membrane Cholesterol ◽  
Frog Neuromuscular Junction ◽  
Vesicle Release ◽  
Synaptic Vesicle Release

scholarly journals Ammonium chloride alters neuronal excitability and synaptic vesicle release

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Roman M. Lazarenko ◽  
Claire E. DelBove ◽  
Claire E. Strothman ◽  
Qi Zhang
Keyword(s):  
Synaptic Vesicle ◽  
Ammonium Chloride ◽  
Neuronal Excitability ◽  
Vesicle Release ◽  
Synaptic Vesicle Release

Spatial variability in release at the frog neuromuscular junction measured with FM1-43

1999 ◽  
Vol 77 (9) ◽  
pp. 672-678 ◽  
Author(s):  
L -G Wu ◽  
W J Betz
Keyword(s):  
Neuromuscular Junction ◽  
Spatial Variability ◽  
Synaptic Vesicle ◽  
Motor Nerve ◽  
Frog Neuromuscular Junction ◽  
Electron Microscopic ◽  
Electrical Nerve Stimulation ◽  
Large Vesicle ◽  
Fluorescence And Electron Microscopy ◽  
Alpha Bungarotoxin

We quantified the spatial variability in release properties at different synaptic vesicle clusters in frog motor nerve terminals, using a combination of fluorescence and electron microscopy. Individual synaptic vesicle clusters labeled with FM1-43 varied more than 10-fold in initial intensity (integrated FM1-43 fluorescence) and in absolute rate of dye loss during tetanic electrical nerve stimulation. Most of this variability arose because large vesicle clusters spanned more than one presynaptic active zone (inferred from postsynaptic acetylcholine receptor stripes labeled with rhodamine-conjugated alpha-bungarotoxin); when the rate of dye loss was normalized to the length of receptor stripe covered, variability from spot to spot was greatly reduced. In addition, electron microscopic measurements showed that large vesicle clusters (i.e., those spanning multiple active zones) were also thicker, and the increased depth of vesicles led to increased total spot fluorescence without a corresponding increase in the rate of dye loss during stimulation. These results did not reveal the presence of "hot zones" of secretory activity.Key words: synaptic transmission, exocytosis, synaptic vesicles, neuromuscular junction.

scholarly journals On the Brink: A New Synaptic Vesicle Release Model at the Calyx of Held

Neuron ◽  
2015 ◽  
Vol 85 (1) ◽  
pp. 6-8 ◽  
Author(s):  
Melissa A. Herman ◽  
Christian Rosenmund
Keyword(s):  
Synaptic Vesicle ◽  
Calyx Of Held ◽  
Vesicle Release ◽  
Synaptic Vesicle Release ◽  
Release Model

scholarly journals Effects of diet on synaptic vesicle release in dynactin complex mutants: a mechanism for improved vitality during motor disease

Aging Cell ◽  
2012 ◽  
Vol 11 (3) ◽  
pp. 418-427 ◽  
Author(s):  
Joel M. Rawson ◽  
Tabita Kreko ◽  
Holly Davison ◽  
Rebekah Mahoney ◽  
Alex Bokov ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Vesicle Release ◽  
Synaptic Vesicle Release ◽  
Dynactin Complex

scholarly journals The Effect of Neural Noise on Spike Time Precision in a Detailed CA3 Neuron Model

2012 ◽  
Vol 2012 ◽  
pp. 1-16 ◽  
Author(s):  
Eduard Kuriscak ◽  
Petr Marsalek ◽  
Julius Stroffek ◽  
Zdenek Wünsch
Keyword(s):  
Synaptic Vesicle ◽  
Neuron Model ◽  
Channel Noise ◽  
Spike Time ◽  
Vesicle Release ◽  
Time Jitter ◽  
Voltage Gated ◽  
Neural Noise ◽  
Quantal Size ◽  
Synaptic Vesicle Release

Experimental and computational studies emphasize the role of the millisecond precision of neuronal spike times as an important coding mechanism for transmitting and representing information in the central nervous system. We investigate the spike time precision of a multicompartmental pyramidal neuron model of the CA3 region of the hippocampus under the influence of various sources of neuronal noise. We describe differences in the contribution to noise originating from voltage-gated ion channels, synaptic vesicle release, and vesicle quantal size. We analyze the effect of interspike intervals and the voltage course preceding the firing of spikes on the spike-timing jitter. The main finding of this study is the ranking of different noise sources according to their contribution to spike time precision. The most influential is synaptic vesicle release noise, causing the spike jitter to vary from 1 ms to 7 ms of a mean value 2.5 ms. Of second importance was the noise incurred by vesicle quantal size variation causing the spike time jitter to vary from 0.03 ms to 0.6 ms. Least influential was the voltage-gated channel noise generating spike jitter from 0.02 ms to 0.15 ms.

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