Molecular characterization and functional analysis of akt‐1 in pinewood nematode, Bursaphelenchus xylophilus

2021 ◽  
Vol 51 (1) ◽  
Author(s):  
Lifeng Zhou ◽  
Jingjing Ji ◽  
Najie Zhu ◽  
Kai Guo ◽  
Jia Tang ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Molecular Characterization ◽  
Bursaphelenchus Xylophilus ◽  
Pinewood Nematode

Molecular characterization and functional analysis of a calponin gene from the pinewood nematode

2015 ◽  
Vol 45 (6) ◽  
pp. 467-473 ◽  
Author(s):  
J. M. S. Cardoso ◽  
L. Fonseca ◽  
P. Gomes ◽  
C. Egas ◽  
I. Abrantes
Keyword(s):  
Functional Analysis ◽  
Molecular Characterization ◽  
Pinewood Nematode

scholarly journals α-l-Fucosidases from Bursaphelenchus xylophilus Secretome—Molecular Characterization and Their Possible Role in Breaking Down Plant Cell Walls

Forests ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 265
Author(s):  
Joana M.S. Cardoso ◽  
Luís Fonseca ◽  
Isabel Abrantes
Keyword(s):  
Cell Wall ◽  
Molecular Characterization ◽  
Three Dimensional ◽  
Pine Wilt Disease ◽  
Bursaphelenchus Xylophilus ◽  
Pinewood Nematode ◽  
Cell Wall Degrading Enzymes ◽  
Degrading Enzymes ◽  
Host Trees ◽  
Resin Canals

The pinewood nematode (PWN) Bursaphelenchus xylophilus, the causal agent of the pine wilt disease (PWD), enters above-ground parts of the tree, migrates through the resin canals and feeds on plant cells causing extensive damage. In order to penetrate the cell wall and establish a parasitic relationship with host trees, the PWN needs to secretea mixture of active cell wall degrading enzymes. In maritime pine, Pinus pinaster, which is high susceptible to PWN, xyloglucan is the major hemicellulosic polysaccharide in primary cells. The xyloglucan backbone is susceptible to hydrolysis by numerous endoglucanases, some of them specific to xyloglucan. However, to completely degrade xyloglucan, all substitutions on the glucan backbones must be released, and l-fucose residues in xyloglucan branches are released by α-l-fucosidases. In the present study, the molecular characterization of two α-l-fucosidases found in PWN secretome was performed. Moreover, a novel α-l-fucosidase was identified and its cDNA and gene sequence were determined. The three-dimensional structures of these α-l-fucosidases were predicted and the transcript levels were analyzed, thus providing new insights into fundamental PWN biology and the possible role of these proteins as cell wall degrading enzymes.

Cloning, Molecular Characterization, Functional Analysis of Wheat TaVIP1 Genes

2017 ◽  
Vol 43 (2) ◽  
pp. 201
Author(s):  
Pei ZHAO ◽  
Li-Jie TENG ◽  
Ke WANG ◽  
Li-Pu DU ◽  
Xian REN ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Molecular Characterization
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