A major facilitator superfamily transporter in Colletotrichum fructicola (CfMfs1) is required for sugar transport, appressorial turgor pressure, conidiation and pathogenicity

2019 ◽  
Vol 49 (6) ◽  
pp. e12558
Author(s):  
Yanying Chen ◽  
Guoying Zhou ◽  
Junang Liu
Keyword(s):  
Turgor Pressure ◽  
Sugar Transport ◽  
Major Facilitator Superfamily ◽  
Colletotrichum Fructicola ◽  
Major Facilitator Superfamily Transporter ◽  
Major Facilitator

scholarly journals The Major Facilitator Superfamily Transporter ZIFL2 Modulates Cesium and Potassium Homeostasis in Arabidopsis

2014 ◽  
Vol 56 (1) ◽  
pp. 148-162 ◽  
Author(s):  
Estelle Remy ◽  
Tânia R. Cabrito ◽  
Rita A. Batista ◽  
Miguel C. Teixeira ◽  
Isabel Sá-Correia ◽  
...  
Keyword(s):  
Major Facilitator Superfamily ◽  
Potassium Homeostasis ◽  
Major Facilitator Superfamily Transporter ◽  
Major Facilitator

scholarly journals Enhanced Efflux Pump Expression in Candida Mutants Results in Decreased Manogepix Susceptibility

2020 ◽  
Vol 64 (5) ◽  
Author(s):  
Sean D. Liston ◽  
Luke Whitesell ◽  
Mili Kapoor ◽  
Karen Joy Shaw ◽  
Leah E. Cowen
Keyword(s):  
Fungal Pathogens ◽  
Candida Parapsilosis ◽  
Efflux Pump ◽  
Major Facilitator Superfamily ◽  
Transporter Gene ◽  
Transcription Factor Gene ◽  
Content Type ◽  
Atp Binding Cassette Transporter ◽  
Major Facilitator Superfamily Transporter ◽  
Major Facilitator

ABSTRACT Manogepix is a broad-spectrum antifungal agent that inhibits glycosylphosphatidylinositol (GPI) anchor biosynthesis. Using whole-genome sequencing, we characterized two efflux-mediated mechanisms in the fungal pathogens Candida albicans and Candida parapsilosis that resulted in decreased manogepix susceptibility. In C. albicans, a gain-of-function mutation in the transcription factor gene ZCF29 activated expression of ATP-binding cassette transporter genes CDR11 and SNQ2. In C. parapsilosis, a mitochondrial deletion activated expression of the major facilitator superfamily transporter gene MDR1.

scholarly journals γ-Resorcylate Catabolic-Pathway Genes in the Soil Actinomycete Rhodococcus jostii RHA1

2015 ◽  
Vol 81 (21) ◽  
pp. 7656-7665 ◽  
Author(s):  
Daisuke Kasai ◽  
Naoto Araki ◽  
Kota Motoi ◽  
Shota Yoshikawa ◽  
Toju Iino ◽  
...  
Keyword(s):  
Major Facilitator Superfamily ◽  
Pcr Analysis ◽  
Decarboxylase Activity ◽  
Content Type ◽  
Reverse Transcription Pcr ◽  
Intergenic Regions ◽  
Major Facilitator Superfamily Transporter ◽  
Maleylacetate Reductase ◽  
Major Facilitator ◽  
Rhodococcus Jostii

ABSTRACTTheRhodococcus jostiiRHA1 gene cluster required for γ-resorcylate (GRA) catabolism was characterized. The cluster includestsdA,tsdB,tsdC,tsdD,tsdR,tsdT, andtsdX, which encode GRA decarboxylase, resorcinol 4-hydroxylase, hydroxyquinol 1,2-dioxygenase, maleylacetate reductase, an IclR-type regulator, a major facilitator superfamily transporter, and a putative hydrolase, respectively. ThetsdAgene conferred GRA decarboxylase activity onEscherichia coli. Purified TsdB oxidized NADH in the presence of resorcinol, suggesting thattsdBencodes a unique NADH-specific single-component resorcinol 4-hydroxylase. Mutations in eithertsdAortsdBresulted in growth deficiency on GRA. ThetsdCandtsdDgenes conferred hydroxyquinol 1,2-dioxygenase and maleylacetate reductase activities, respectively, onE. coli. Inactivation oftsdTsignificantly retarded the growth of RHA1 on GRA. The growth retardation was partially suppressed under acidic conditions, suggesting the involvement oftsdTin GRA uptake. Reverse transcription-PCR analysis revealed that thetsdgenes constitute three transcriptional units, thetsdBADCandtsdTXoperons andtsdR. Transcription of thetsdBADCandtsdTXoperons was induced during growth on GRA. Inactivation oftsdRderepressed transcription of thetsdBADCandtsdTXoperons in the absence of GRA, suggesting thattsdgene transcription is negatively regulated by thetsdR-encoded regulator. Binding of TsdR to thetsdR-tsdBandtsdT-tsdRintergenic regions was inhibited by the addition of GRA, indicating that GRA interacts with TsdR as an effector molecule.

scholarly journals Aspergillus niger mstA encodes a high-affinity sugar/H+ symporter which is regulated in response to extracellular pH

2004 ◽  
Vol 379 (2) ◽  
pp. 375-383 ◽  
Author(s):  
Patricia A. vanKUYK ◽  
Jasper A. DIDERICH ◽  
Andrew P. MacCABE ◽  
Oscar HERERRO ◽  
George J. G. RUIJTER ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Ph Regulation ◽  
Functional Characterization ◽  
Carbon Sources ◽  
Sugar Transport ◽  
Major Facilitator Superfamily ◽  
Northern Analysis ◽  
High Affinity ◽  
Hexose Uptake ◽  
Major Facilitator

A sugar-transporter-encoding gene, mstA, which is a member of the major facilitator superfamily, has been cloned from a genomic DNA library of the filamentous fungus Aspergillus niger. To enable the functional characterization of MSTA, a full-length cDNA was expressed in a Saccharomyces cerevisiae strain deficient in hexose uptake. Uptake experiments using 14C-labelled monosaccharides demonstrated that although able to transport d-fructose (Km, 4.5±1.0 mM), d-xylose (Km, 0.3±0.1 mM) and d-mannose (Km, 60±20 µM), MSTA has a preference for d-glucose (Km, 25±10 µM). pH changes associated with sugar transport indicate that MSTA catalyses monosaccharide/H+ symport. Expression of mstA in response to carbon starvation and upon transfer to poor carbon sources is consistent with a role for MSTA as a high-affinity transporter for d-glucose, d-mannose and d-xylose. Northern analysis has shown that mstA is subject to CreA-mediated carbon catabolite repression and pH regulation mediated by PacC. A. niger strains in which the mstA gene had been disrupted are phenotypically identical with isogenic reference strains when grown on 0.1–60 mM d-glucose, d-mannose, d-fructose or d-xylose. This indicates that A. niger possesses other transporters capable of compensating for the absence of MSTA.

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