Characterization of cuticular compounds of the cerambycid beetles Monochamus galloprovincialis , Arhopalus syriacus , and Pogonocherus perroudi , potential vectors of pinewood nematode

2021 ◽  
Vol 169 (2) ◽  
pp. 183-194
Author(s):  
Elsa Gonçalves ◽  
Ana Cristina Figueiredo ◽  
José G. Barroso ◽  
Jocelyn G. Millar ◽  
Joana Henriques ◽  
...  
Keyword(s):  
Pinewood Nematode ◽  
Monochamus Galloprovincialis ◽  
Cuticular Compounds ◽  
Cerambycid Beetles

scholarly journals Molecular and biochemical characterization of an endo-β-1,3-glucanase from the pinewood nematode Bursaphelenchus xylophilus acquired by horizontal gene transfer from bacteria

2005 ◽  
Vol 389 (1) ◽  
pp. 117-125 ◽  
Author(s):  
Taisei KIKUCHI ◽  
Hajime SHIBUYA ◽  
John T. JONES
Keyword(s):  
Gene Transfer ◽  
Horizontal Gene Transfer ◽  
Biochemical Characterization ◽  
Glycosyl Hydrolase ◽  
Functional Characterization ◽  
Nematode Species ◽  
Bursaphelenchus Xylophilus ◽  
Pinewood Nematode ◽  
Sequence Comparisons

We report the cloning and functional characterization of an endo-β-1,3-glucanase from the pinewood nematode Bursaphelenchus xylophilus acquired by horizontal gene transfer from bacteria. This is the first gene of this type from any nematode species. We show that a similar cDNA is also present in another closely related species B. mucronatus, but that similar sequences are not present in any other nematode studied to date. The B. xylophilus gene is expressed solely in the oesophageal gland cells of the nematode and the protein is present in the nematode's secretions. The deduced amino acid sequence of the gene is very similar to glycosyl hydrolase family 16 proteins. The recombinant protein, expressed in Escherichia coli, preferentially hydrolysed the β-1,3-glucan laminarin, and had very low levels of activity on β-1,3-1,4-glucan, lichenan and barley β-glucan. Laminarin was degraded in an endoglucanase mode by the enzyme. The optimal temperature and pH for activity of the recombinant enzyme were 65 °C and pH 4.9. The protein is probably important in allowing the nematodes to feed on fungi. Sequence comparisons suggest that the gene encoding the endo-β-1,3-glucanase was acquired by horizontal gene transfer from bacteria. B. xylophilus therefore contains genes that have been acquired by this process from both bacteria and fungi. These findings support the idea that multiple independent horizontal gene transfer events have helped in shaping the evolution of several different life strategies in nematodes.

Presence of the cerambycid beetles Psacothea hilaris and Monochamus alternatus affecting the life cycle strategy of Bursaphelenchus xylophilus

Nematology ◽  
2001 ◽  
Vol 3 (5) ◽  
pp. 455-461 ◽  
Author(s):  
Noritoshi Maehara ◽  
Kazuyoshi Futai
Keyword(s):  
Life History ◽  
Bursaphelenchus Xylophilus ◽  
Pinewood Nematode ◽  
Fourth Stage ◽  
Monochamus Alternatus ◽  
The Third ◽  
Life Cycle Strategy ◽  
Pine Trees ◽  
History Of ◽  
Cerambycid Beetles

AbstractAlthough fourth-stage dispersal juveniles (JIV) of Bursaphelenchus xylophilus, the pinewood nematode (PWN), developed in the presence of both Monochamus alternatus and Psacothea hilaris, the numbers and the percentage of JIV were far higher in the presence of the former than of the latter. JIV first appeared 7 days after pupation of M. alternatus and the number increased from the day of beetle eclosion to the third day thereafter, then remained stable. We conclude that the presence of specific vectors affects both the life history of the PWN and the numbers of nematodes carried by vectors emerging from killed pine trees.

Precise characterization of cuticular compounds in youngDrosophila by mass spectrometry

1988 ◽  
Vol 14 (4) ◽  
pp. 1071-1085 ◽  
Author(s):  
J. M. Pechine ◽  
C. Antony ◽  
J. -M. Jallon
Keyword(s):  
Mass Spectrometry ◽  
Cuticular Compounds ◽  
Precise Characterization

Phylogeography of Monochamus galloprovincialis, the European vector of the pinewood nematode

2017 ◽  
Vol 91 (1) ◽  
pp. 247-257 ◽  
Author(s):  
Julien Haran ◽  
Jérôme Rousselet ◽  
David Tellez ◽  
Alain Roques ◽  
Géraldine Roux
Keyword(s):  
Pinewood Nematode ◽  
Monochamus Galloprovincialis

scholarly journals Identification and Characterization of a Dual-Acting Antinematodal Agent against the Pinewood Nematode, Bursaphelenchus xylophilus

PLoS ONE ◽  
2009 ◽  
Vol 4 (11) ◽  
pp. e7593 ◽  
Author(s):  
Wan-Suk Oh ◽  
Pan-Young Jeong ◽  
Hyoe-Jin Joo ◽  
Jeong-Eui Lee ◽  
Yil-Seong Moon ◽  
...  
Keyword(s):  
Bursaphelenchus Xylophilus ◽  
Pinewood Nematode ◽  
Identification And Characterization

scholarly journals Characterization of Bacteria Associated with Pinewood Nematode Bursaphelenchus xylophilus

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e46661 ◽  
Author(s):  
Claudia S. L. Vicente ◽  
Francisco Nascimento ◽  
Margarida Espada ◽  
Pedro Barbosa ◽  
Manuel Mota ◽  
...  
Keyword(s):  
Bursaphelenchus Xylophilus ◽  
Pinewood Nematode

Characterization of the relationships in the pinewood nematode species complex (PWNSC) (Bursaphelenchus spp.) using a heterologous unc-22 DNA probe from Caenorhabditis elegans

Parasitology ◽  
1991 ◽  
Vol 102 (2) ◽  
pp. 303-308 ◽  
Author(s):  
P. Abad ◽  
S. Tares ◽  
N. Brugier ◽  
G. De Guiran
Keyword(s):  
Species Complex ◽  
Nematode Species ◽  
Bursaphelenchus Xylophilus ◽  
Similar Species ◽  
Pinewood Nematode ◽  
C Elegans ◽  
The World ◽  
Different Populations ◽  
Serious Disease

SUMMARYPine wilt is the most serious disease of native pines in Japan and potentially the most important nematode disease of conifers in the world. The pinewood nematode Bursaphelenchus xylophilus was found to be the causal agent. Difficulties arose with respect to the precise identity of some isolates of B. xylophilus and of similar species B. mucronatus and B. fraudulentus. Restriction enzyme analyses of repetitive DNA revealed bands specific for the species B. xylophilus, B. mucronatus and B. fraudulentus. Hybridization patterns obtained with unc-22 gene of C. elegans, clearly identified B. xylophilus, B. mucronatus and B. fraudulentus as well as the different geographic isolates of these species. Furthermore, it is possible to define the phylogenetic relationships between the different populations constituting the ‘pine wood nematode’ complex.

Sign in / Sign up

Export Citation Format

Share Document