scholarly journals The parasitic fly Nemorilla maculosa exploits host-plant volatiles to locate the legume pod borer, Maruca vitrata

2018 ◽  
Vol 166 (8) ◽  
pp. 673-682
Author(s):  
Ayaovi Agbessenou ◽  
Agbéko Kodjo Tounou ◽  
Elie Ayitondji Dannon ◽  
Benjamin Datinon ◽  
Cyriaque Agboton ◽  
...  
Keyword(s):  
Host Plant ◽  
Plant Volatiles ◽  
Maruca Vitrata ◽  
Host Plant Volatiles ◽  
Pod Borer ◽  
Legume Pod Borer

scholarly journals Functional characteristics of a novel odorant binding protein in the legume pod borer, Maruca vitrata (Lepidoptera: Crambidae)

2021 ◽  
Author(s):  
Hui Ai ◽  
Yuying Liu ◽  
Guangyan Long ◽  
Yuan Yuan ◽  
Shaopei Huang ◽  
...  
Keyword(s):  
Host Plant ◽  
Binding Protein ◽  
Butyl Ester ◽  
Acid Sites ◽  
Maruca Vitrata ◽  
Odorant Binding Protein ◽  
Olfactory Sensilla ◽  
Pod Borer ◽  
Legume Pod Borer ◽  
Odorant Binding

Abstract Insect olfaction system plays a key role in the foraging food, pollination, mating, oviposition, reproduction and other insect physiological behavior. Odorant binding protein are widely found in the various olfactory sensilla of different insect antennae and involved in chemical signals discrimination from natural environment. In this study, a novel OBP gene, MvitOBP3 is identified from the legume pod borer, Maruca vitrata, which it mainly harms important legume vegetables including cowpea, soybean and lablab bean. Real-time PCR results demonstrated that MvitOBP3 gene was abundantly expressed in the antennal tissue of M. vitrata, while low levels were distributed in the head, thorax, abdomen, leg and wing of adult moths. The recombinant OBP3 protein was purified using the prokaryotic expression and affinity chromatography system. Fluorescence competitive binding experiments indicated that that MvitOBP3 protein exhibited greater binding affinities with host-plant flower volatiles including Butanoic acid butyl ester, Limonene, 1H-indol-4-ol and 2-methyl-3-phenylpropanal, highlighting they may have attractant activities for the oviposition of female moths on the legume vegetables. Moreover, protein homology modeling and molecular docking analysis revealed that there are six amino acid sites of MvitOBP3 involved in the binding of the host-plant volatiles. These findings will further promote to understand the key role of odorant binding protein during host perception and oviposition of M. vitrata moths, which improve the efficiency of semiochemical-based prevention and monitoring for this pest in the legume vegetables field.

scholarly journals Functional characteristics of a novel odorant binding protein in the legume pod borer, Maruca vitrata

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hui Ai ◽  
Yuying Liu ◽  
Guangyan Long ◽  
Yuan Yuan ◽  
Shaopei Huang ◽  
...  
Keyword(s):  
Host Plant ◽  
Binding Protein ◽  
Butyl Ester ◽  
Acid Sites ◽  
Maruca Vitrata ◽  
Odorant Binding Protein ◽  
Olfactory Sensilla ◽  
Pod Borer ◽  
Legume Pod Borer ◽  
Odorant Binding

AbstractInsect olfaction system plays a key role in the foraging food, pollination, mating, oviposition, reproduction and other insect physiological behavior. Odorant binding protein are widely found in the various olfactory sensilla of different insect antennae and involved in chemical signals discrimination from natural environment. In this study, a novel OBP gene, MvitOBP3 is identified from the legume pod borer, Maruca vitrata, which it mainly harms important legume vegetables including cowpea, soybean and lablab bean. Real-time PCR results demonstrated that MvitOBP3 gene was abundantly expressed in the antennal tissue of M. vitrata, while low levels were distributed in the head, thorax, abdomen, leg and wing of adult moths. The recombinant OBP3 protein was purified using the prokaryotic expression and affinity chromatography system. Fluorescence competitive binding experiments indicated that that MvitOBP3 protein exhibited greater binding affinities with host-plant flower volatiles including Butanoic acid butyl ester, Limonene, 1H-indol-4-ol and 2-methyl-3-phenylpropanal, highlighting they may have attractant activities for the oviposition of female moths on the legume vegetables. Moreover, protein homology modeling and molecular docking analysis revealed that there are six amino acid sites of MvitOBP3 involved in the binding of the host-plant volatiles. These findings will further promote to understand the key role of odorant binding protein during host perception and oviposition of M. vitrata moths, which improve the efficiency of semiochemical-based prevention and monitoring for this pest in the legume vegetables field.

Infectivity of entomopathogenic nematodes against the legume pod-borer, Maruca vitrata Fabricius, infesting pigeon pea

2021 ◽  
Vol 95 ◽  
Author(s):  
R. Pervez ◽  
U. Rao
Keyword(s):  
Entomopathogenic Nematodes ◽  
Agricultural Research ◽  
Insect Pest ◽  
Indian Agricultural Research Institute ◽  
Pigeon Pea ◽  
Fourth Instar ◽  
Maruca Vitrata ◽  
Pod Borer ◽  
Legume Pod Borer ◽  
Maximum Penetration

Abstract The legume pod-borer, Maruca vitrata Fabricius (Lepidoptera: Crambidae) (LPB), is an important insect pest of pigeon pea. Chemical pesticides are generally employed to manage this pest, but because of the soil residue issues and other environmental hazards associated with their use, biopesticides are also in demand. Another benign alternative is to use entomopathogenic nematodes (EPNs) to manage this vital pest. In the present study, the infectivity of ten native EPNs was evaluated against LPB by assessing their penetration and production in the LPB. The effectiveness of the promising EPNs against second-, third- and fourth-instar LPB larvae was also studied. Heterorhabditis sp. (Indian Agricultural Research Institute-Entomopathogenic Nematodes Rashid Pervez (IARI-EPN RP) 06) and Oscheius sp. (IARI-EPN RP 08) were found to be most pathogenic to LPB, resulting in about 100% mortality within 72 h, followed by Steinernema sp. (IARI-EPN RP 03 and 09). Oscheius sp. (IARI-EPN RP 04) was found to be the least pathogenic to LPB larva with 67% mortality. Maximum penetration was exhibited by Heterorhabditis sp. (IARI-EPN RP 06) followed by Oscheius sp. (IARI-EPN RP 08), whereas the lowest rate of penetration was exhibited by Oscheius sp. (IARI-EPN RP 01). The highest rate of production was observed with Oscheius sp. (IARI-EPN RP 08), followed by Oscheius sp. (IARI-EPN RP 04 and 10). Among the tested instars of the LPB larvae, second-instar larvae were more susceptible to EPNs, followed by third- and fourth-instar larvae. The results indicate that Heterorhabditis sp. (IARI-EPN RP 06) and Oscheius sp. (IARI-EPN RP 08) have a good potential to the manage LPB.

Transgenic cowpea plants expressing Bacillus thuringiensis Cry2Aa insecticidal protein imparts resistance to Maruca vitrata legume pod borer

2021 ◽  
Vol 40 (3) ◽  
pp. 583-594
Author(s):  
Anil Kumar ◽  
Ranjana Jaiwal ◽  
Rohini Sreevathsa ◽  
Darshna Chaudhary ◽  
Pawan K. Jaiwal
Keyword(s):  
Bacillus Thuringiensis ◽  
Maruca Vitrata ◽  
Insecticidal Protein ◽  
Pod Borer ◽  
Legume Pod Borer ◽  
Transgenic Cowpea
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