Responses by the brown planthopper,Nilaparvata lugens, to conspecific density on resistant and susceptible rice varieties

2016 ◽  
Vol 158 (3) ◽  
pp. 284-294 ◽  
Author(s):  
Finbarr G. Horgan ◽  
Bhaskar S. Naik ◽  
Eko Hari Iswanto ◽  
Maria Liberty P. Almazan ◽  
Angelee Fame Ramal ◽  
...  
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Rice Varieties ◽  
Conspecific Density

scholarly journals Detection of Yeast-like Symbionts in Brown Planthopper Reared on Different Resistant Rice Varieties Combining DGGE and Absolute Quantitative Real-Time PCR

Insects ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 85
Author(s):  
Chengling Lai ◽  
Yun Hou ◽  
Peiying Hao ◽  
Kun Pang ◽  
Xiaoping Yu
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Quantitative Real Time Pcr ◽  
Nymphal Instar ◽  
Rice Varieties ◽  
Development Stages ◽  
The Third ◽  
Minimum Number

The brown planthopper (BPH), Nilaparvata lugens, is a serious pest of rice throughout Asia. Yeast-like symbionts (YLS) are endosymbionts closely linked with the development of BPH and the adapted mechanism of BPH virulence to resistant plants. In this study, we used semi-quantitative DGGE and absolute quantitative real-time PCR (qPCR) to quantify the number of the three YLS strains (Ascomycetes symbionts, Pichia-like symbionts, and Candida-like symbionts) that typically infect BPH in the nymphal stages and in newly emerged female adults. The quantities of each of the three YLS assessed increased in tandem with the developing nymphal instar stages, peaking at the fourth instar stage, and then declined significantly at the fifth instar stage. However, the amount of YLS present recovered sharply within the emerging adult females. Additionally, we estimated the quantities of YLS for up to eight generations after their inoculation onto resistant cultivars (Mudgo, ASD7, and RH) to reassociate the dynamics of YLS with the fitness of BPH. The minimum number of each YLS was detected in the second generation and gradually increased from the third generation with regard to resistant rice varieties. In addition, the Ascomycetes symbionts of YLS were found to be the most abundant of the three YLS strains tested for all of the development stages of BPH.

scholarly journals Ketahanan padi (Oryza sativa L.) varietas IPB 3S terhadap wereng batang cokelat (Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

2020 ◽  
Vol 17 (2) ◽  
pp. 97
Author(s):  
Andi Nurdaaniyah ◽  
Dadang Dadang ◽  
I Wayan Winasa
Keyword(s):  
Sex Ratio ◽  
Insect Pests ◽  
Oryza Sativa L ◽  
Feeding Activity ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Population Increase ◽  
Rice Varieties ◽  
High Loss ◽  
Rice Plants

<p>Brown planthopper (<em>Nilaparvata lugens</em> (Stål)) is one of the major rice insect pests that is known to cause high loss of rice production. One of the strategies to control this pest is by using resistant rice varieties. The aim of this research was to study the resistance of IPB 3S variety to BPH by measuring BPH fecundity, sex ratio, feeding activity as well as population increase compared to Ciherang variety against brown planthopper (<em>N. lugens</em>). Fecundity test used a pair of BPH adult infested on 21 days after planting (DAP) of rice plants. Eggs laid in rice stalks and the rest of eggs in the ovary were counted. In order to know the change of sex ratio, a pair of BPH adults was infested on 30 DAP rice plants and allowed to lay eggs. The calculation of sex ratio was conducted at adult stage of BPH by segregation between male and female adults. Feeding activity of BPH was evaluated using ninhydrin and analyzed qualitatively using spectrophotometer. Ten fourth instar nymphs of BPH was infested on 30 DAP old of rice seedlings. Honeydew secreted by BPH was collected to filter paper sprayed with ninhydrin. Population increased test was conducted by infesting five pairs of BPH adults on 35 DAP old rice plants. Observations was conducted on nymph and adult stages. Each test was replicated 10 times. In general, IPB 3S is slightly resistant to BPH, compared to Ciherang variety. The feeding activity of BPH is higher in Ciherang compared to IPB3S. However, the fecundity and population increased of BPH in Ciherang is similar to IPB3S.</p>

scholarly journals Bacterial Symbionts of the Brown Planthopper, Nilaparvata lugens (Homoptera: Delphacidae)

2010 ◽  
Vol 76 (6) ◽  
pp. 1740-1745 ◽  
Author(s):  
Ming Tang ◽  
Lu Lv ◽  
Shengli Jing ◽  
Lili Zhu ◽  
Guangcun He
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Rrna Gene ◽  
Rice Varieties ◽  
Ovipositor Sheath ◽  
Operational Taxonomic Units ◽  
Metaseiulus Occidentalis ◽  
High Virulence ◽  
Gene Libraries

ABSTRACT The brown planthopper (Nilaparvata lugens Stål), the most destructive pest of rice, has been identified, including biotypes with high virulence towards previously resistant rice varieties. There have also been many reports of a yeast-like symbiont of N. lugens, but little is known about the bacterial microbes. In this study, we examined the bacterial microbes in N. lugens and identified a total of 18 operational taxonomic units (OTUs) representing four phyla (Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes) by sequencing and analyzing 16S rRNA gene libraries obtained from three populations of N. lugens, which were maintained on the rice varieties TN1, Mudgo, and ASD7. Several of the OTUs were similar to previously reported secondary symbionts of other insects, including an endosymbiont of the psyllid Glycapsis brimblecombei, an Asaia sp. found in the mosquito Anopheles stephensi, and Wolbachia, found in the mite Metaseiulus occidentalis. However, the species and numbers of the detected OTUs differed substantially among the N. lugens populations. Further, in situ hybridization analysis using digoxigenin-labeled probes indicated that OTU 1 was located in hypogastrium tissues near the ovipositor and ovary in biotype 1 insects, while OTU 2 was located in the front of the ovipositor sheath in biotype 2 insects. In addition, masses of bacterium-like organisms were observed in the tubes of salivary sheaths in rice plant tissues that the insects had fed upon. The results provide indications of the diversity of the bacterial microbes harbored by the brown planthopper and of possible associations between specific bacterial microbes and biotypes of N. lugens.

scholarly journals Recent Strategies for Detection and Improvement of Brown Planthopper Resistance Genes in Rice: A Review

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1202
Author(s):  
Bello Sani Haliru ◽  
Mohd Y. Rafii ◽  
Norida Mazlan ◽  
Shairul Izan Ramlee ◽  
Isma’ila Muhammad ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Large Scale ◽  
Insect Pest ◽  
Durable Resistance ◽  
Brown Planthopper ◽  
Gene Clusters ◽  
Nilaparvata Lugens ◽  
Scale Production ◽  
Rice Varieties ◽  
Resistant Genes

Brown planthopper (BPH; Nilaparvata lugens Stal) is considered the main rice insect pest in Asia. Several BPH-resistant varieties of rice have been bred previously and released for large-scale production in various rice-growing regions. However, the frequent surfacing of new BPH biotypes necessitates the evolution of new rice varieties that have a wide genetic base to overcome BPH attacks. Nowadays, with the introduction of molecular approaches in varietal development, it is possible to combine multiple genes from diverse sources into a single genetic background for durable resistance. At present, above 37 BPH-resistant genes/polygenes have been detected from wild species and indica varieties, which are situated on chromosomes 1, 3, 4, 6, 7, 8, 9, 10, 11 and 12. Five BPH gene clusters have been identified from chromosomes 3, 4, 6, and 12. In addition, eight BPH-resistant genes have been successfully cloned. It is hoped that many more resistance genes will be explored through screening of additional domesticated and undomesticated species in due course.

Feeding responses of the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), to resistant and susceptible host-plants

1989 ◽  
Vol 79 (2) ◽  
pp. 309-318 ◽  
Author(s):  
D. E. Padgham ◽  
S. Woodhead ◽  
H. R. Rapusas
Keyword(s):  
Resistance Genes ◽  
Host Plants ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Resistance Mechanisms ◽  
Population Development ◽  
Rice Varieties ◽  
Susceptible Host ◽  
Insect Activity ◽  
Nilaparvata Lugens Stål

AbstractThe feeding responses, growth and population development of Nilaparvata lugens (Stål) are quantified on a range of twelve susceptible or resistant host-plants. Paris of rice varieties carrying the Bph1, bph2 and Bph3 resistance genes are compared as hosts for N. lugens, and it is concluded that such notations do not adequately describe the diversity of plant resistance mechanisms. Evidence is presented for resistance mechanisms involving enhanced insect activity and gustatory responses to unacceptable phloem.

scholarly journals EFFECTS OF POPULATION DENSITY AND HOST AVAILABILITY ON THE MIGRATION PROCESS OF BROWN PLANTHOPPER FED USING SUSCEPTIBLE AND RESISTANT RICE VARIETIES (PENGARUH KEPADATAN POPULASI DAN KETERSEDIAAN PAKAN TERHADAP PROSES MIGRASI WERENG BATANG COKELAT PADA PADI VARIETAS RENTAN DAN TAHAN)

2017 ◽  
Vol 20 (2) ◽  
pp. 72
Author(s):  
Imam Habibi ◽  
Witjaksono Witjaksono ◽  
Arman Wijonarko
Keyword(s):  
Population Density ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Susceptible Variety ◽  
Resistant Variety ◽  
Rice Varieties ◽  
Host Availability ◽  
Important Pest ◽  
Nilaparvata Lugens Stål ◽  
Field Failure

Brown planthopper, Nilaparvata lugens Stal. (Hemiptera: Delphacidae), is an important pest of rice. This pest can cause hopperburn and field failure. This research aimed to determine the effects of population density and host availability on migration of N. lugens. The criteria used to justify the effects of host availability and population density on migration of N. lugens were based the hardness and tannin tests of the rice stems, fecundity of N. lugens, and the life cycle of N. lugens. The research was conducted under the temperature of 29.42°C with relative humidity of 61% and Light 12: Dark 12 times, using ten pairs of N. lugens brachypterous (F0 constant) and then was added with five male adults on fifth days after the first infestation (F0 changed). The varieties used were IR64, as a resistant variety, and Ketan Lusi, as a susceptible variety. The results showed that the adding of the macropterous males did not affect the number of macropterous, because of that has been preplanned by the F0. Therefore, the percentage of existing macropterous was 51−52%. Wereng Batang Cokelat (WBC) merupakan salah satu hama tanaman padi yang sangat penting. Kerusakan parah dapat menyebabkan hopperburn dan puso (gagal panen). Tujuan penelitian ini adalah mengetahui pengaruh kepadatan populasi dan tanaman inang sebagai tempat migrasi WBC. Parameter yang dikaji untuk mengetahui pengaruh kepadatan populasi WBC dan tanaman inang tempat migrasi WBC berdasarkan tingkat kekerasan dan kandungan tanin batang tanaman padi, fekunditas WBC, dan siklus hidup WBC. Penelitian ini dilakukan pada temperatur 29.42˚C dengan kelembapan relatif 61% dan durasi siang hari 12 jam: durasi malam hari 12 jam. Metode yang dilakukan adalah dengan menggunakan 10 pasang imago WBC brakhiptera (F0 konstan), kemudian dilakukan penambahan 5 ekor imago jantan pada hari kelima setelah infestasi awal (F0 diubah). Varietas padi yang digunakan yaitu padi varietas IR64 sebagai varietas tahan dan ketan Lusi sebagai varietas rentan. Hasil penelitian menunjukkan bahwa penambahan imago jantan makroptera tidak berpengaruh terhadap jumlah keturunan makroptera yang dihasilkan, karena imago (F0) telah merencanakan terlebih dahulu keturunan yang akan dihasilkan. Oleh karena itu, persentase terbentuk keturunan imago makroptera berkisar antara 51−52%. 

scholarly journals Brown Planthopper Nilaparvata lugens STÅL (Homoptera: Delphacidae) Biotypes Capable of Attacking Resistant Rice Varieties in Malaysia

1994 ◽  
Vol 29 (4) ◽  
pp. 523-532 ◽  
Author(s):  
Kiyomitsu ITO ◽  
Takashi WADA ◽  
Akihiko TAKAHASHI ◽  
Nik Salleh NOOR ◽  
Hassim HABIBUDDlN
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Rice Varieties
Sign in / Sign up

Export Citation Format

Share Document