Comparative evaluation of the clonogenic capacity of periodontal ligament fibroblasts in Hank's balanced salt solution and egg albumen: An in vitro study

2018 ◽  
Vol 34 (4) ◽  
pp. 278-283
Author(s):  
Dimpi Shah ◽  
Prasad Sulkshane ◽  
Rutika Lalwani ◽  
Sagar Pawar ◽  
Tanuja Teni ◽  
...  
Keyword(s):  
Salt Solution ◽  
Periodontal Ligament ◽  
Comparative Evaluation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Periodontal Ligament Fibroblasts ◽  
Egg Albumen

scholarly journals Comparative evaluation of the effect of cold ceramic and MTA-Angelus on cell viability, attachment and differentiation of dental pulp stem cells and periodontal ligament fibroblasts: an in vitro study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sedigheh Khedmat ◽  
Pegah Sarraf ◽  
Ehsan Seyedjafari ◽  
Parisa Sanaei-rad ◽  
Faranak Noori
Keyword(s):  
Cell Viability ◽  
Dental Pulp ◽  
Periodontal Ligament ◽  
In Vitro Study ◽  
Vitro Study ◽  
Control Group ◽  
Periodontal Ligament Fibroblasts ◽  
Pulp Therapy ◽  
Vital Pulp Therapy

Abstract Background Biocompatibility and induction of mineralized tissue formation are the properties expected from a material used in vital pulp therapy and repair of perforations. Cold ceramic (SJM, Iran; CC) is a newly introduced calcium silicate-based cement for above mentioned therapeutic applications. This in-vitro study aimed to compare the effect of CC and White MTA-Angelus (MTA) on cell viability, attachment, odontogenic differentiation, and calcification potential of human dental pulp stem cells (DPSCs) and periodontal ligament fibroblasts (PDLFs). Methods Cell viability of DPSCs and PDLFs was assessed using MTT on days 1, 3, 7, and 14 (n = 9) in contact with freshly mixed and set states of CC and MTA. Field emission scanning electron micrographs (FESEM) were taken to evaluate cell-bioceramic interaction (n = 6). Gene expression levels of osteo/odontogenic markers (Dentin sialophosphoprotein, Dentin matrix protein 1, Collagen type I alpha 1, and Alkaline phosphatase (DSPP, DMP1, COL 1A1, and ALP, respectively) (n = 8) were assessed using qrt-PCR. ALP enzymatic activity was evaluated to assess the mineralization potential. A two-way ANOVA test was applied, and p < 0.05 was considered to be statistically significant. Results The effect of freshly mixed and set MTA and CC on the survival of DPSCs and PDLFs in all study groups was statistically similar and comparable to the positive control group (p > 0.05); the only exception was for the viability of PDLFs in contact with freshly mixed cements on day 1, showing a more significant cytotoxic effect compared to the control and the set state of materials (p < 0.05). PDLFs attached well on CC and MTA. The spread and pseudopodium formation of the cells increased on both samples from day 1 to day 14. Contact of MTA and CC with DPSCs similarly increased expression of all dentinogenesis markers studied on days 7 and 14 compared to the control group (p < 0.001), except for DSPP expression on day 7 (p = 0.46 and p = 0.99 for MTA and CC, respectively). Conclusions Within the limitation of this in-vitro study, cold ceramic and MTA-Angelus showed high biocompatibility and induced increased expression of osteo/dentinogenic markers. Therefore, cold ceramic can be a suitable material for vital pulp therapy and the repair of root perforations.

Sign in / Sign up

Export Citation Format

Share Document