Coconut milk and probiotic milk as storage media to maintain periodontal ligament cell viability: an in vitro study

2016 ◽  
Vol 33 (3) ◽  
pp. 160-164 ◽  
Author(s):  
Divya Saini ◽  
Prahlad Gadicherla ◽  
Prakash Chandra ◽  
Latha Anandakrishna
Keyword(s):  
Cell Viability ◽  
Periodontal Ligament ◽  
In Vitro Study ◽  
Coconut Milk ◽  
Vitro Study ◽  
Periodontal Ligament Cell ◽  
Storage Media

scholarly journals Dragon's Blood Sap (Croton Lechleri) As Storage Medium For Avulsed Teeth: In Vitro Study Of Cell Viability

2016 ◽  
Vol 27 (6) ◽  
pp. 751-756 ◽  
Author(s):  
Christine Men Martins ◽  
Elizane Ferreira Hamanaka ◽  
Thayse Yumi Hoshida ◽  
Ana Maria Sell ◽  
Mirian Marubayashi Hidalgo ◽  
...  
Keyword(s):  
Cell Viability ◽  
Periodontal Ligament ◽  
Trypan Blue ◽  
In Vitro Study ◽  
Vitro Study ◽  
Periodontal Ligament Cells ◽  
Storage Medium ◽  
Dragon’S Blood ◽  
Storage Media

Abstract Tooth replantation success depends on the condition of cementum periodontal ligament after tooth avulsion; which is influenced by storage medium. The dragon's blood (Croton lechleri) sap has been suggested as a promising medium because it supports collagen formation and exhibits healing, anti-inflammatory and antimicrobial properties. Thus, the aim of this study was to evaluate the efficacy of dragon's blood sap as a storage medium for avulsed teeth through evaluation of functional and metabolic cell viability. This in vitro study compared the efficacy of different storage media to maintain the viability of human peripheral blood mononuclear and periodontal ligament cells. A 10% dragon's blood sap was tested while PBS was selected as its control. Ultra pasteurized whole milk was used for comparison as a commonly used storage medium. DMEM and distilled water were the positive and negative controls, respectively. The viability was assessed through trypan blue exclusion test and colorimetric MTT assay after 1, 3, 6, 10 and 24 h of incubation. The dragon's blood sap showed promising results due to its considerable maintenance of cell viability. For trypan blue test, the dragon's blood sap was similar to milk (p<0.05) and both presented the highest viability values. For MTT, the dragon's blood sap showed better results than all storage media, even better than milk (p<0.05). It was concluded that the dragon's blood sap was as effective as milk, the gold standard for storage medium. The experimental sap preserved the membrane of all cells and the functional viability of periodontal ligament cells.

scholarly journals Comparative evaluation of the effect of cold ceramic and MTA-Angelus on cell viability, attachment and differentiation of dental pulp stem cells and periodontal ligament fibroblasts: an in vitro study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sedigheh Khedmat ◽  
Pegah Sarraf ◽  
Ehsan Seyedjafari ◽  
Parisa Sanaei-rad ◽  
Faranak Noori
Keyword(s):  
Cell Viability ◽  
Dental Pulp ◽  
Periodontal Ligament ◽  
In Vitro Study ◽  
Vitro Study ◽  
Control Group ◽  
Periodontal Ligament Fibroblasts ◽  
Pulp Therapy ◽  
Vital Pulp Therapy

Abstract Background Biocompatibility and induction of mineralized tissue formation are the properties expected from a material used in vital pulp therapy and repair of perforations. Cold ceramic (SJM, Iran; CC) is a newly introduced calcium silicate-based cement for above mentioned therapeutic applications. This in-vitro study aimed to compare the effect of CC and White MTA-Angelus (MTA) on cell viability, attachment, odontogenic differentiation, and calcification potential of human dental pulp stem cells (DPSCs) and periodontal ligament fibroblasts (PDLFs). Methods Cell viability of DPSCs and PDLFs was assessed using MTT on days 1, 3, 7, and 14 (n = 9) in contact with freshly mixed and set states of CC and MTA. Field emission scanning electron micrographs (FESEM) were taken to evaluate cell-bioceramic interaction (n = 6). Gene expression levels of osteo/odontogenic markers (Dentin sialophosphoprotein, Dentin matrix protein 1, Collagen type I alpha 1, and Alkaline phosphatase (DSPP, DMP1, COL 1A1, and ALP, respectively) (n = 8) were assessed using qrt-PCR. ALP enzymatic activity was evaluated to assess the mineralization potential. A two-way ANOVA test was applied, and p < 0.05 was considered to be statistically significant. Results The effect of freshly mixed and set MTA and CC on the survival of DPSCs and PDLFs in all study groups was statistically similar and comparable to the positive control group (p > 0.05); the only exception was for the viability of PDLFs in contact with freshly mixed cements on day 1, showing a more significant cytotoxic effect compared to the control and the set state of materials (p < 0.05). PDLFs attached well on CC and MTA. The spread and pseudopodium formation of the cells increased on both samples from day 1 to day 14. Contact of MTA and CC with DPSCs similarly increased expression of all dentinogenesis markers studied on days 7 and 14 compared to the control group (p < 0.001), except for DSPP expression on day 7 (p = 0.46 and p = 0.99 for MTA and CC, respectively). Conclusions Within the limitation of this in-vitro study, cold ceramic and MTA-Angelus showed high biocompatibility and induced increased expression of osteo/dentinogenic markers. Therefore, cold ceramic can be a suitable material for vital pulp therapy and the repair of root perforations.

scholarly journals In Vitro Periodontal Ligament Cell Viability in Different Storage Media

2016 ◽  
Vol 27 (4) ◽  
pp. 408-411 ◽  
Author(s):  
Meenakshi Sharma
Keyword(s):  
Cell Viability ◽  
Periodontal Ligament ◽  
Egg White ◽  
Periodontal Ligament Cells ◽  
Periodontal Ligament Cell ◽  
Trypan Blue Exclusion ◽  
Storage Media ◽  
Significant Difference ◽  
Rice Water

Abstract The aim of this study was to evaluate the viability of periodontal ligament cells of avulsed teeth in three different storage media. Forty-five mature premolars extracted for orthodontic therapeutic purposes were randomly and equally divided into three groups according to the storage medium: milk (control), rice water and egg white. After placing extracted teeth for 30 min in storage media, the scrapings of the periodontal ligament (PDL) were collected in Falcon tubes containing collagenase in 2.5 mL of phosphate buffer saline and were incubated for 30 min and centrifuged for 5 min at 800 rpm. Cell viability was analyzed by Trypan blue exclusion. Rice water had a significantly higher number of viable cells compared to egg white and milk. There was no statistically significant difference between egg white and milk. Rice water may be able to maintain PDL cell viability of avulsed teeth better than egg white or milk.

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