scholarly journals The use of mice in diabetes research: the impact of experimental protocols

2021 ◽  
Author(s):  
M.R. Kennard ◽  
L.F. Daniels Gatward ◽  
A. Roberts ◽  
E. White ◽  
M. Nandi ◽  
...  
Keyword(s):  
Diabetes Research ◽  
Experimental Protocols ◽  
The Impact

scholarly journals Treatment Tone Spacing and Acute Effects of Acoustic Coordinated Reset Stimulation in Tinnitus Patients

2021 ◽  
Vol 1 ◽  
Author(s):  
Tina Munjal ◽  
Alexander N. Silchenko ◽  
Kristina J. Pfeifer ◽  
Summer S. Han ◽  
Jessica K. Yankulova ◽  
...  
Keyword(s):  
Animal Studies ◽  
Symptomatic Improvement ◽  
Center Frequency ◽  
Acute Effects ◽  
Stimulus Amplitude ◽  
Neuronal Populations ◽  
Experimental Protocols ◽  
Relationship Of ◽  
The Impact ◽  
Coordinated Reset

Acoustic coordinated reset (aCR) therapy for tinnitus aims to desynchronize neuronal populations in the auditory cortex that exhibit pathologically increased coincident firing. The original therapeutic paradigm involves fixed spacing of four low-intensity tones centered around the frequency of a tone matching the tinnitus pitch, fT, but it is unknown whether these tones are optimally spaced for induction of desynchronization. Computational and animal studies suggest that stimulus amplitude, and relatedly, spatial stimulation profiles, of coordinated reset pulses can have a major impact on the degree of desynchronization achievable. In this study, we transform the tone spacing of aCR into a scale that takes into account the frequency selectivity of the auditory system at each therapeutic tone’s center frequency via a measure called the gap index. Higher gap indices are indicative of more loosely spaced aCR tones. The gap index was found to be a significant predictor of symptomatic improvement, with larger gap indices, i.e., more loosely spaced aCR tones, resulting in reduction of tinnitus loudness and annoyance scores in the acute stimulation setting. A notable limitation of this study is the intimate relationship of hearing impairment with the gap index. Particularly, the shape of the audiogram in the vicinity of the tinnitus frequency can have a major impact on tone spacing. However, based on our findings we suggest hypotheses-based experimental protocols that may help to disentangle the impact of hearing loss and tone spacing on clinical outcome, to assess the electrophysiologic correlates of clinical improvement, and to elucidate the effects following chronic rather than acute stimulation.

Lactate delivery (not oxygen) limits hepatic gluconeogenesis when blood flow is reduced

2006 ◽  
Vol 290 (1) ◽  
pp. E192-E198 ◽  
Author(s):  
Ken D. Sumida ◽  
Jerry H. Urdiales ◽  
Casey M. Donovan
Keyword(s):  
Blood Flow ◽  
Flow Rate ◽  
Oxygen Delivery ◽  
Liver Perfusion ◽  
Lactate Concentration ◽  
Oxygen Availability ◽  
Limiting Factor ◽  
Hepatic Gluconeogenesis ◽  
Experimental Protocols ◽  
The Impact

The purpose of this study was to determine, using the isolated liver perfusion technique, whether the limiting factor for hepatic gluconeogenesis (GNG) from lactate was precursor delivery or oxygen availability during reduced flow rates of 0.85 or 0.60 ml·min−1·g liver−1. After a 24-h fast, three different experimental protocols were employed. Protocol 1 examined the impact on GNG when reservoir lactate concentration was maintained but oxygen delivery was elevated via increases in hematocrit (Hct). Elevating the Hct from 22.5 ± 0.8% to 30.9 ± 0.4% at a blood flow of 0.89 ± 0.01 ml·min−1·g liver−1 increased the oxygen consumption (V̇o2) but did not augment GNG. Similarly, when the Hct was elevated from 22.5 ± 0.8% to 41.5 ± 0.7% at 0.59 ± 0.04 ml·min−1·g liver−1, V̇o2 was increased, but GNG was unaffected. Protocol 2 examined the impact on GNG when Hct was maintained but precursor delivery was elevated via increases in reservoir lactate concentration ([LA]). Specifically, elevating the [LA] from 2.31 ± 0.07 to 3.61 ± 0.33 mM at a flow rate of 0.82 ± 0.04 ml·min−1·g liver−1 significantly increased GNG. Similarly, elevating the [LA] from 2.31 ± 0.07 to 4.24 ± 0.37 mM at a flow rate of 0.58 ± 0.02 ml·min−1·g liver−1 increased GNG. Finally, we examined the impact of increasing both the oxygen and lactate delivery ( Protocol 3). Again, V̇o2 was elevated with increased oxygen delivery, but GNG was not augmented beyond that observed with elevations in lactate delivery alone, i.e., Protocol 2. The results indicate that, during decrements in blood flow, GNG is limited primarily by precursor delivery, not oxygen availability.

scholarly journals Changes in Physiological and Pathological Behaviours Produced by Deep Microelectrode Implantation Surgery in Rats: A Temporal Analysis

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Gustavo A. Chiprés-Tinajero ◽  
Miguel A. Núñez-Ochoa ◽  
Laura Medina-Ceja
Keyword(s):  
Animal Models ◽  
Neurological Diseases ◽  
Behavioural Change ◽  
Temporal Analysis ◽  
Exploratory Activity ◽  
Male Wistar Rats ◽  
Experimental Protocols ◽  
Convulsive Seizures ◽  
The Impact ◽  
Mean Time

Physiological behaviours such as the sleep-wake cycle and exploratory behaviours are important parameters in intact and sham-operated animals and are usually thought to be unaffected by experimental protocols in which neurosurgery is performed. However, there is insufficient evidence in the literature on the behavioural and cognitive effects observed after deep microelectrode implantation surgery in animal models of neurological diseases. Similarly, in studies that utilize animal models of neurological diseases, the impact of surgery on the pathological phenomena being studied is often minimized. Based on these considerations, we performed a temporal analysis of the effects of deep microelectrode implantation surgery in the hippocampus of rats on quiet wakefulness, sleep, and exploratory activity and the pathological behaviours such as convulsive seizures according to the Racine scale. Male Wistar rats (210-300 g) were used and grouped in sham and epileptic animals. Single doses of pilocarpine hydrochloride (2.4 mg/2 μl; i.c.v.) were administered to the animals to generate spontaneous and recurrent seizures. Deep microelectrode implantation surgeries in both groups and analysis of Fast ripples were performed. Physiological and pathological behaviours were recorded through direct video monitoring of animals (24/7). Our principal findings showed that in epileptic animals, one of the main behaviours affected by surgery is sleep; as a consequence of this behavioural change, a decrease in exploratory activity was also found as well as the mean time spent daily in seizures of scale 4 and the number of seizure events of scales 4 and 5 was increased after surgery. No significant correlations between the occurrence of FR and seizure events of scale 4 (rho 0.63, p value 0.25) or 5 (rho -0.7, p value 0.18) were observed. In conclusion, microelectrode implantation surgeries modified some physiological and pathological behaviours; therefore, it is important to consider this fact when it is working with animal models.

scholarly journals The Impact of Single-Cell Genomics on Adipose Tissue Research

2020 ◽  
Vol 21 (13) ◽  
pp. 4773
Author(s):  
Alana Deutsch ◽  
Daorong Feng ◽  
Jeffrey E. Pessin ◽  
Kosaku Shinoda
Keyword(s):  
Adipose Tissue ◽  
Single Cell ◽  
Energy Homeostasis ◽  
Whole Body ◽  
Diabetes Research ◽  
Obesity And Diabetes ◽  
Important Regulator ◽  
Whole Body Metabolism ◽  
Tissue Aging ◽  
The Impact

Adipose tissue is an important regulator of whole-body metabolism and energy homeostasis. The unprecedented growth of obesity and metabolic disease worldwide has required paralleled advancements in research on this dynamic endocrine organ system. Single-cell RNA sequencing (scRNA-seq), a highly meticulous methodology used to dissect tissue heterogeneity through the transcriptional characterization of individual cells, is responsible for facilitating critical advancements in this area. The unique investigative capabilities achieved by the combination of nanotechnology, molecular biology, and informatics are expanding our understanding of adipose tissue’s composition and compartmentalized functional specialization, which underlie physiologic and pathogenic states, including adaptive thermogenesis, adipose tissue aging, and obesity. In this review, we will summarize the use of scRNA-seq and single-nuclei RNA-seq (snRNA-seq) in adipocyte biology and their applications to obesity and diabetes research in the hopes of increasing awareness of the capabilities of this technology and acting as a catalyst for its expanded use in further investigation.

scholarly journals On the impact of different types of errors on trust in human-robot interaction

2019 ◽  
Vol 20 (3) ◽  
pp. 455-486 ◽  
Author(s):  
Rebecca Flook ◽  
Anas Shrinah ◽  
Luc Wijnen ◽  
Kerstin Eder ◽  
Chris Melhuish ◽  
...  
Keyword(s):  
Human Robot Interaction ◽  
General Effect ◽  
Laboratory Studies ◽  
Subjective Measures ◽  
Robot Interaction ◽  
Independent Laboratory ◽  
Different Types ◽  
Experimental Protocols ◽  
The Impact

Abstract Trust is a key dimension of human-robot interaction (HRI), and has often been studied in the HRI community. A common challenge arises from the difficulty of assessing trust levels in ecologically invalid environments: we present in this paper two independent laboratory studies, totalling 160 participants, where we investigate the impact of different types of errors on resulting trust, using both behavioural and subjective measures of trust. While we found a (weak) general effect of errors on reported and observed level of trust, no significant differences between the type of errors were found in either of our studies. We discuss this negative result in light of our experimental protocols, and argue for the community to move towards alternative methodologies to assess trust.

scholarly journals Central Nervous System Manifestations in Diabetes Mellitus - A Review

2017 ◽  
Vol 18 (2) ◽  
pp. 109-112
Author(s):  
Goutam Kumar Acherjya ◽  
Md Moslem Uddin ◽  
MA Jalil Chowdhury ◽  
AV Srinivasan
Keyword(s):  
Diabetes Mellitus ◽  
Central Nervous System ◽  
Nervous System ◽  
Stroke Risk ◽  
Diabetes Research ◽  
Seizure Disorders ◽  
Oral Agents ◽  
Hyperosmolar Coma ◽  
The Impact ◽  
The Brain

The impact of diabetes mellitus on the CNS (Central Nervous System) has gained attention only recently. Peripheral neuropathy has been the primary neuroscience focus of diabetes research. Contrary to some early impressions, however, the CNS is not spared by diabetes. Chronically, diabetes mellitus affects the CNS in several ways. Diabetes increases stroke risk and damage, overtreatment with insulin or oral agents can permanently damage the brain, and diabetes may increase the prevalence of seizure disorders. Diabetes changes brain transport, blood flow and metabolism, and may produce a chronic encephalopathy. Acutely, glycemic extremes cause coma, seizures, focal neurolclgical deficits, and impaired consciousness. The pathophysiological basis for these marked CNS abnormalities seen in hypoglycemia, hyperosmolar coma, and ketoacidosis are largely unknown.Methods: This review was based on a search of Pubmed, the NCBI Database of systemic Reviews, and citation lists of relevant publications. Subject heading and key words used central nervous system, diabetes mellitus, stroke, encephalopathy and hypoglycaemia. Only articles in English were included.J MEDICINE July 2017; 18 (2) : 109-112

scholarly journals How to Best Protect People With Diabetes From the Impact of SARS-CoV-2: Report of the International COVID-19 and Diabetes Summit

2021 ◽  
pp. 193229682097839
Author(s):  
Jennifer Y. Zhang ◽  
Trisha Shang ◽  
David Ahn ◽  
Kong Chen ◽  
Gerard Coté ◽  
...  
Keyword(s):  
New Technologies ◽  
Digital Health ◽  
Risk Groups ◽  
Diabetes Research ◽  
Government Officials ◽  
Global Pandemic ◽  
Diabetes Technology ◽  
The Future ◽  
Treatment Of Diabetes ◽  
The Impact

The coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus has rapidly involved the entire world and exposed the pressing need for collaboration between public health and other stakeholders from the clinical, scientific, regulatory, pharmaceutical, and medical device and technology communities. To discuss how to best protect people with diabetes from serious outcomes from COVID-19, Diabetes Technology Society, in collaboration with Sansum Diabetes Research Institute, hosted the “International COVID-19 and Diabetes Virtual Summit” on August 26-27, 2020. This unique, unprecedented real-time conference brought together physicians, scientists, government officials, regulatory experts, industry representatives, and people with diabetes from six continents to review and analyze relationships between COVID-19 and diabetes. Over 800 attendees logged in. The summit consisted of five sessions: (I) Keynotes, (II) Preparedness, (III) Response, (IV) Recovery, and (V) Surveillance; eight parts: (A) Background, (B) Resilience, (C) Outpatient Care, (D) Inpatient Care, (E) Resources, (F) High-Risk Groups, (G) Regulation, and (H) The Future; and 24 sections: (1) Historic Pandemics and Impact on Society, (2) Pathophysiology/Risk Factors for COVID-19, (3) Social Determinants of COVID-19, (4) Preparing for the Future, (5) Medications and Vaccines, (6) Psychology of Patients and Caregivers, (7) Outpatient Treatment of Diabetes Mellitus and Non-Pharmacologic Intervention, (8) Technology and Telehealth for Diabetes Outpatients, (9) Technology for Inpatients, (10) Management of Diabetes Inpatients with COVID-19, (11) Ethics, (12) Accuracy of Diagnostic Tests, (13) Children, (14) Pregnancy, (15) Economics of Care for COVID-19, (16) Role of Industry, (17) Protection of Healthcare Workers, (18) People with Diabetes, (19) International Responses to COVID-19, (20) Government Policy, (21) Regulation of Tests and Treatments, (22) Digital Health Technology, (23) Big Data Statistics, and 24) Patient Surveillance and Privacy. The two keynote speeches were entitled (1) COVID-19 and Diabetes—Meeting the Challenge and (2) Knowledge Gaps and Research Opportunities for Diabetes and COVID-19. While there was an emphasis on diabetes and its interactions with COVID-19, the panelists also discussed the COVID-19 pandemic in general. The meeting generated many novel ideas for collaboration between experts in medicine, science, government, and industry to develop new technologies and disease treatment paradigms to fight this global pandemic.

scholarly journals The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

2020 ◽  
Vol 66 (8) ◽  
pp. 1012-1029 ◽  
Author(s):  
Jim F Huggett ◽  
◽  
Alexandra S Whale ◽  
Ward De Spiegelaere ◽  
Wim Trypsteen ◽  
...  
Keyword(s):  
Digital Pcr ◽  
Experimental Information ◽  
Minimum Information ◽  
Software Applications ◽  
Technological Potential ◽  
Experimental Protocols ◽  
Experimental Process ◽  
Threshold Setting ◽  
The Impact ◽  
Process Adoption

Abstract Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology.

scholarly journals Impact of Vector Priming on the Immunogenicity of Recombinant Salmonella Vaccines

2003 ◽  
Vol 71 (1) ◽  
pp. 287-297 ◽  
Author(s):  
Christofer J. Vindurampulle ◽  
Stephen R. Attridge
Keyword(s):  
Escherichia Coli ◽  
Immunoglobulin G ◽  
Salmonella Enterica ◽  
Lymphoid Tissue ◽  
Antibody Responses ◽  
Vaccine Strategy ◽  
Foreign Antigen ◽  
Experimental Protocols ◽  
The Impact ◽  
Intestinal Iga

ABSTRACT There are conflicting reports concerning the impact of prior vector priming on the immunogenicity of recombinant-Salmonella-based vaccines. A comparison of experimental protocols identified two variables which might account for this inconsistency: the potential of the vector strain to colonize the murine gut-associated lymphoid tissue (GALT) and the nature of the foreign antigen subsequently delivered by the recombinant Salmonella construct. The former was investigated by constructing an aroA mutant of the Salmonella enterica serovar Stanley vector previously used in our laboratory. Although the introduction of an aroA mutation had surprisingly little effect on GALT colonization, it did reduce the strength of antilipopolysaccharide (anti-LPS) antibody responses and the impact of vector priming. Studies were also performed to ascertain the extent to which any observed hyporesponsiveness consequent upon vector priming might be determined by the characteristics of the foreign antigen. S. enterica serovar Stanley was used to deliver either of two Escherichia coli antigens, K88 pilus protein or the LT-B toxin subunit, to vector-primed mice. Both serum immunoglobulin G (IgG) and intestinal IgA responses to K88 were completely abolished, and those to LT-B were significantly reduced, as a consequence of vector priming. When similar experiments were performed with an aroA S. enterica serovar Dublin vector, responses to K88 were significantly reduced but those to LT-B were unaffected by vector priming. Paradoxically, a priming infection with this vector induced stronger anti-LPS antibody responses but was less likely to elicit a state of hyporesponsiveness to subsequently presented foreign antigen. The impact of vector priming thus depends on both the Salmonella strain used and the nature of the foreign antigen, but our present data strengthen concerns that preexisting antivector immunity represents a serious threat to the Salmonella-based vaccine strategy.

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