scholarly journals HbA 1c determination from HemaSpot™ blood collection devices: comparison of home prepared dried blood spots with standard venous blood analysis

2019 ◽  
Vol 37 (9) ◽  
pp. 1463-1470 ◽  
Author(s):  
J. M. Hall ◽  
C. F. Fowler ◽  
F. Barrett ◽  
R. W. Humphry ◽  
M. Van Drimmelen ◽  
...  
Keyword(s):  
Venous Blood ◽  
Dried Blood Spots ◽  
Blood Analysis ◽  
Blood Collection ◽  
Blood Spots

scholarly journals Detection of Stimulated Erythropoiesis by the RNA-Based 5'-Aminolevulinate Synthase 2 Biomarker in Dried Blood Spot Samples

2019 ◽  
Vol 65 (12) ◽  
pp. 1563-1571 ◽  
Author(s):  
Olivier Salamin ◽  
Emeric Gottardo ◽  
Céline Schobinger ◽  
Gemma Reverter-Branchat ◽  
Jordi Segura ◽  
...  
Keyword(s):  
Recombinant Human Erythropoietin ◽  
Hematological Parameters ◽  
Venous Blood ◽  
Dried Blood Spots ◽  
Storage Conditions ◽  
Blood Collection ◽  
Aminolevulinate Synthase ◽  
Human Erythropoietin ◽  
Biological Passport ◽  
New Biomarkers

Abstract BACKGROUND Despite implementation of the Athlete Biological Passport 10 years ago, blood doping remains difficult to detect. Thus, there is a need for new biomarkers to increase the sensitivity of the adaptive model. Transcriptomic biomarkers originating from immature reticulocytes may be reliable indicators of blood manipulations. Furthermore, the use of dried blood spots (DBSs) for antidoping purposes constitutes a complementary approach to venous blood collection. Here, we developed a method of quantifying the RNA-based 5′-aminolevulinate synthase 2 (ALAS2) biomarker in DBS. MATERIALS The technical, interindividual, and intraindividual variabilities of the method, and the effects of storage conditions on the production levels of ALAS2 RNA were assessed. The method was used to monitor erythropoiesis stimulated endogenously (blood withdrawal) or exogenously (injection of recombinant human erythropoietin). RESULTS When measured over a 7-week period, the intra- and interindividual variabilities of ALAS2 expression in DBS were 12.5%–42.4% and 49%, respectively. Following withdrawal of 1 unit of blood, the ALAS2 RNA in DBS increased significantly for up to 15 days. Variations in the expression level of this biomarker in DBS samples were more marked than those of the conventional hematological parameters, reticulocyte percentage and immature reticulocyte fraction. After exogenous stimulation of erythropoiesis via recombinant human erythropoietin injection, ALAS2 expression in DBS increased by a mean 8-fold. CONCLUSIONS Monitoring of transcriptomic biomarkers in DBS could complement the measurement of hematological parameters in the Athlete Biological Passport and aid the detection of blood manipulations.

scholarly journals A field trial of a PCR-based Mansonella ozzardi diagnosis assay detects high-levels of submicroscopic M. ozzardi infections in both venous blood samples and FTA® card dried blood spots

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Jansen Fernandes Medeiros ◽  
Tatiana Amaral Pires Almeida ◽  
Lucyane Bastos Tavares Silva ◽  
Jose Miguel Rubio ◽  
James Lee Crainey ◽  
...  
Keyword(s):  
Field Trial ◽  
Venous Blood ◽  
Dried Blood Spots ◽  
Blood Samples ◽  
Blood Spots

An Assessment of Dried Blood-Spot Technology for Identifying Iron Deficiency

Blood ◽  
1998 ◽  
Vol 92 (5) ◽  
pp. 1807-1813 ◽  
Author(s):  
James D. Cook ◽  
Carol H. Flowers ◽  
Barry S. Skikne
Keyword(s):  
Iron Deficiency ◽  
Transferrin Receptor ◽  
Venous Blood ◽  
Dried Blood Spots ◽  
Epidemiologic Studies ◽  
Capillary Blood ◽  
Deficiency Anemia ◽  
Blood Samples ◽  
Blood Spots ◽  
American Society

Abstract The present study was undertaken to assess the feasibility of using ferritin and transferrin receptor measurements on dried capillary blood spots to identify iron deficiency (ID) in public health surveys. Measurements on serum and blood spots prepared from venous blood were performed in 71 healthy subjects, 41 of whom were iron-replete and 30 who had ID, either without (n = 20) or with (n = 10) anemia. Parallel measurements were performed on hemolyzed whole blood and washed hemolyzed red blood cells to assess the erythrocyte contribution of ferritin and transferrin receptor to dried blood samples. The concentration of ferritin in dried blood samples was threefold higher than serum assays due to the release of ferritin from hemolyzed erythrocytes, which diminished the usefulness of ferritin measurements for detecting ID. On the other hand, there was negligible erythrocyte contribution to the measurement of transferrin receptor in dried blood spots. The most sensitive parameter in dried blood spots was the ratio of receptor/ferritin, which was suitable for identifying iron-deficiency anemia (IDA), but less reliable than serum assays for detecting milder ID without anemia. We conclude that tandem measurements of serum ferritin and transferrin receptor in dried blood spots can be used to facilitate the identification of IDA in epidemiologic studies. © 1998 by The American Society of Hematology.

Contamination of dried blood spots – an underestimated risk in newborn screening

2018 ◽  
Vol 56 (2) ◽  
pp. 278-284 ◽  
Author(s):  
Theresa Winter ◽  
Anja Lange ◽  
Anke Hannemann ◽  
Matthias Nauck ◽  
Cornelia Müller
Keyword(s):  
Newborn Screening ◽  
Infant Formula ◽  
False Positive ◽  
Dried Blood Spots ◽  
Substantial Effect ◽  
Blood Collection ◽  
Inborn Errors ◽  
Blood Spots ◽  
Filter Papers ◽  
The Impact

Abstract Background: Newborn screening (NBS) is an established screening procedure in many countries worldwide, aiming at the early detection of inborn errors of metabolism. For decades, dried blood spots have been the standard specimen for NBS. The procedure of blood collection is well described and standardized and includes many critical pre-analytical steps. We examined the impact of contamination of some anticipated common substances on NBS results obtained from dry spot samples. This possible pre-analytical source of uncertainty has been poorly examined in the past. Methods: Capillary blood was obtained from 15 adult volunteers and applied to 10 screening filter papers per volunteer. Nine filter papers were contaminated without visible trace. The contaminants were baby diaper rash cream, baby wet wipes, disinfectant, liquid infant formula, liquid infant formula hypoallergenic (HA), ultrasonic gel, breast milk, feces, and urine. The differences between control and contaminated samples were evaluated for 45 NBS quantities. We estimated if the contaminations might lead to false-positive NBS results. Results: Eight of nine investigated contaminants significantly altered NBS analyte concentrations and potentially caused false-positive screening outcomes. A contamination with feces was most influential, affecting 24 of 45 tested analytes followed by liquid infant formula (HA) and urine, affecting 19 and 13 of 45 analytes, respectively. Conclusions: A contamination of filter paper samples can have a substantial effect on the NBS results. Our results underline the importance of good pre-analytical training to make the staff aware of the threat and ensure reliable screening results.

scholarly journals Value of Phenylalanine in Amniotic Fluid Detected by MS/MS for Screening of ma ternal PKU in the Second-Trimester

2020 ◽  
Author(s):  
yanyun wang ◽  
yun Sun ◽  
yu-guo Wang ◽  
tao Jiang
Keyword(s):  
Amniotic Fluid ◽  
Reference Values ◽  
Venous Blood ◽  
Dried Blood Spots ◽  
Unknown Etiology ◽  
Case Group ◽  
Multiple Gestation ◽  
Second Trimester ◽  
Maternal Phenylketonuria ◽  
Blood Spots

Abstract Background: Phenylketonuria is the most common inherited metabolism disease in China. This study aimed to identify a new, sensitive, simple, and easy method of screening maternal Phenylketonuria in the fetal stage.Methods: Samples of amniotic fluid, dried blood spots, and blood collected on the same day were obtained from women in the second-trimester (16–28 weeks). Women had to meet the following criteria: (i) isolated high risk of quadruple marker screening or cell-free DNA screening, (ii) women who gave birth to at least one child with intellectual disability of unknown etiology, and (iii) women who gave birth to a child who died young from unknown etiology. The exclusion criteria were as follows: clinically evident chorioamnionitis, multiple gestation, and laboratory signs of infection in the amniotic fluid sample. Phenylalanine levels were measured using tandem mass spectrometry. Women who met criterion (i) served as controls whose results were used to set reference values of Phenylalanine in amniotic fluid. Women who met criteria (ii) and (iii) served as the case group whose results were used to check for maternal Phenylketonuria. The Spearman rank correlation test was used to analyze the correlation of Phenylalanine in amniotic fluid and in venous blood. Results: We analyzed 365 samples of amniotic fluid. Among them, 345 were included in the control group, with reference values of Phenylalanine in amniotic fluid of 10.79–48.47 µmol/L. Twenty cases were included in the case group. One woman in the case group was diagnosed with hyperphenylalaninemia whose fetus was diagnosed with maternal Phenylketonuria by comprehensive analysis of Phenylalanine in dried blood spots and in amniotic fluid, head circumference in fetal ultrasound, and a previous history of adverse pregnancy. The correlation between Phenylalanine concentrations in venous blood and in amniotic fluid was weak.Conclusions: Phenylalanine can be readily measured in amniotic fluid. Relative to controls, amniotic fluid levels of Phenylalanine was increased in maternal Phenylketonuria. These results can support the use of Phenylalanine as a screening tool for maternal Phenylketonuria. The significance of these change requires further study.

scholarly journals Evaluation of DNA extracted from blood filter spots and eluates processed for enzyme linked immunosorbent assay (ELISA)

2019 ◽  
Author(s):  
Mark Andy Xatse ◽  
Jewelna Akorli ◽  
Irene Offei Owusu ◽  
Livingstone Gati ◽  
Michael David Wilson
Keyword(s):  
Filter Paper ◽  
Dried Blood Spots ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Collection ◽  
Dna Concentration ◽  
Blood Spots ◽  
Molecular Assays ◽  
Blood Filter ◽  
Spin Column ◽  
The 1960S

AbstractDried filter blood spots have become a significant blood collection method for screening individuals for clinical purposes. When used for ELISAs, they are normally discarded after the blood has been eluted. However, they may still be useful for extraction of DNA for molecular-based assays. The aim of this work was to determine the integrity of DNA extracted from filter paper spots from which blood has initially been eluted for ELISA with sample dilution buffer (SDB) and phosphate buffered saline (PBS). DNA was extracted from the eluted filter spots, the eluate, and dried blood filter spots (controls) using spin column extraction. The quality and quantity of the extracted DNA was assessed and used for PCR to further evaluate their usefulness in molecular assays. Concentration of DNA obtained was dependent on the buffer used for processing the filter blood blots. Accounting for the DNA concentration obtained from dried blood spots, which were used as controls, DNA extracted from the already eluted blood spots were 32 times higher in PBS than SDB processed filter paper. The ratio was even higher for the eluates, which were 57 times higher in PBS than SDS eluates. SDB eluates had significantly higher average DNA concentration than their eluted filter paper, but their purity ratios were similar. 85% PCR success rate was achieved with the DNA samples. Useful DNA can be extracted from blood spots after it has been eluted with SDB. Although the DNA concentration and purity may be low, the DNA could be useful for rather simple PCR assays.Author SummaryCollection of blood onto filter paper has become an accepted method for screening individuals for clinical and public health purposes since the 1960s. This method of blood collection has become increasingly popular due to its ease and convenience in collection and transportation. The use of dried blood spots for clinical evaluations and research has become very significant. For research purposes, DBS when used for ELISAs are discarded after single use. DNA may however be extracted from the used filter blots and used for molecular assays. The concentration of DNA obtained may be low but simple assays like PCR could be done using the DNA extracted from the eluted filter spot.

Sign in / Sign up

Export Citation Format

Share Document