Expression of the core promoter factors TATA box binding protein and TATA box binding protein‐related factor 2 in Drosophila germ cells and their distinct functions in germline development

2020 ◽  
Vol 62 (9) ◽  
pp. 540-553
Author(s):  
Shoichi Nakamura ◽  
Seiji Hira ◽  
Makoto Kojima ◽  
Akane Kondo ◽  
Masanori Mukai
Keyword(s):  
Germ Cells ◽  
Binding Protein ◽  
Core Promoter ◽  
Tata Box ◽  
Related Factor ◽  
Germline Development ◽  
The Core ◽  
Tata Box Binding Protein

scholarly journals Variations in Intracellular Levels of TATA Binding Protein Can Affect Specific Genes by Different Mechanisms

2007 ◽  
Vol 28 (1) ◽  
pp. 83-92 ◽  
Author(s):  
Stephanie D. Bush ◽  
Patricia Richard ◽  
James L. Manley
Keyword(s):  
Binding Protein ◽  
Core Promoter ◽  
Tata Binding Protein ◽  
Tata Box ◽  
Wild Type ◽  
The Core ◽  
Mitotic Delay ◽  
Reduced Activity ◽  
Dt40 Cells ◽  
Repressor Element

ABSTRACT We previously showed that reduced intracellular levels of the TATA binding protein (TBP), brought about by tbp heterozygosity in DT40 cells, resulted in a mitotic delay reflecting reduced expression of the mitotic regulator cdc25B but did not significantly affect overall transcription. Here we extend these findings in several ways. We first provide evidence that the decrease in cdc25B expression reflects reduced activity of the cdc25B core promoter in the heterozygous (TBP-het) cells. Strikingly, mutations in a previously described repressor element that overlaps the TATA box restored promoter activity in TBP-het cells, supporting the idea that the sensitivity of this promoter to TBP levels reflects a competition between TBP and the repressor for DNA binding. To determine whether cells might have mechanisms to compensate for fluctuations in TBP levels, we next examined expression of the two known vertebrate TBP homologues, TLP and TBP2. Significantly, mRNAs encoding both were significantly overexpressed relative to levels observed in wild-type cells. In the case of TLP, this was shown to reflect regulation of the core promoter by both TBP and TLP. Together, our results indicate that variations in TBP levels can affect the transcription of specific promoters in distinct ways, but overall transcription may be buffered by corresponding alterations in the expression of TBP homologues.

scholarly journals TRF3, a TATA-box-binding protein-related factor, is vertebrate-specific and widely expressed

2003 ◽  
Vol 100 (25) ◽  
pp. 14887-14891 ◽  
Author(s):  
S. P. Persengiev ◽  
X. Zhu ◽  
B. L. Dixit ◽  
G. A. Maston ◽  
E. L. W. Kittler ◽  
...  
Keyword(s):  
Binding Protein ◽  
Tata Box ◽  
Related Factor ◽  
Tata Box Binding Protein

scholarly journals Differential Utilization of TATA Box-binding Protein (TBP) and TBP-related Factor 1 (TRF1) at Different Classes of RNA Polymerase III Promoters

2013 ◽  
Vol 288 (38) ◽  
pp. 27564-27570 ◽  
Author(s):  
Neha Verma ◽  
Ko-Hsuan Hung ◽  
Jin Joo Kang ◽  
Nermeen H. Barakat ◽  
William E. Stumph
Keyword(s):  
Rna Polymerase ◽  
Binding Protein ◽  
Rna Polymerase Iii ◽  
Tata Box ◽  
Related Factor ◽  
U6 Snrna ◽  
Polymerase Iii ◽  
Tata Box Binding Protein ◽  
In Cells

In the fruit fly Drosophila melanogaster, RNA polymerase III transcription was found to be dependent not upon the canonical TATA box-binding protein (TBP) but instead upon the TBP-related factor 1 (TRF1) (Takada, S., Lis, J. T., Zhou, S., and Tjian, R. (2000) Cell 101, 459–469). Here we confirm that transcription of fly tRNA genes requires TRF1. However, we unexpectedly find that U6 snRNA gene promoters are occupied primarily by TBP in cells and that knockdown of TBP, but not TRF1, inhibits U6 transcription in cells. Moreover, U6 transcription in vitro effectively utilizes TBP, whereas TBP cannot substitute for TRF1 to promote tRNA transcription in vitro. Thus, in fruit flies, different classes of RNA polymerase III promoters differentially utilize TBP and TRF1 for the initiation of transcription.

scholarly journals Initiation of zebrafish haematopoiesis by the TATA-box-binding protein-related factor Trf3

Nature ◽  
2007 ◽  
Vol 450 (7172) ◽  
pp. 1082-1085 ◽  
Author(s):  
Daniel O. Hart ◽  
Tamal Raha ◽  
Nathan D. Lawson ◽  
Michael R. Green
Keyword(s):  
Binding Protein ◽  
Tata Box ◽  
Related Factor ◽  
Tata Box Binding Protein

scholarly journals Polymerase (Pol) III TATA Box-Binding Protein (TBP)-Associated Factor Brf Binds to a Surface on TBP Also Required for Activated Pol II Transcription

1998 ◽  
Vol 18 (3) ◽  
pp. 1692-1700 ◽  
Author(s):  
Yuhong Shen ◽  
George A. Kassavetis ◽  
Gene O. Bryant ◽  
Arnold J. Berk
Keyword(s):  
Gene Transcription ◽  
Transcription Initiation ◽  
Binding Protein ◽  
Tata Box ◽  
Related Factor ◽  
In Vitro Synthesis ◽  
Pol Ii ◽  
U6 Snrna ◽  
Tata Box Binding Protein ◽  
Pol Iii

ABSTRACT The TATA box-binding protein (TBP) plays an essential role in transcription by all three eukaryotic nuclear RNA polymerases, polymerases (Pol) I, II, and III. In each case, TBP interacts with class-specific TBP-associated factors (TAFs) to form class-specific transcription initiation factors. For yeast Pol III transcription, TBP associates with Brf (from TFIIB-related factor) and B", two Pol III TAFs, to form Pol III transcription factor TFIIIB. Here, we identify TBP surface residues that are required for interaction with yeast Pol III TAFs. Ninety-one human TBP surface residue mutants with radical substitutions were analyzed for the ability to form stable gel shift complexes with purified Brf and B" and for their activities for in vitro synthesis of yeast U6 snRNA. Mutations in a large positively charged epitope extending from the top (that is, on the surface opposite the DNA-facing “saddle” of TBP) and onto the side of the first TBP repeat inhibited binding to Brf (residues K181, L185, R186, E206, R231, L232, R235, K236, R239, Q242, K243, K249, and F250). A triple-mutant TBP (R231E + R235E + R239S) had greatly reduced activity for yeast U6 snRNA gene transcription while remaining active for Pol II basal transcription. Similar results were observed when selected mutations were introduced into yeast TBP at equivalent positions. A C-terminal fragment of Brf lacking the region of homology with TFIIB retains the ability to bind TBP-DNA complexes (G. Kassavetis, C. Bardeleben, A. Kumar, E. Ramirez, and E. P. Geiduschek, Mol. Cell. Biol. 17:5299–5306, 1997); the same TBP mutations reduced binding by this fragment. Mutations in TBP residues that interact with TFIIB did not affect Brf binding or U6 gene transcription. These results indicate that Brf and TFIIB interact differently with TBP. An extensively overlapping epitope on the top surface of TBP was found previously to be required for activated Pol II transcription and has been hypothesized to interact with Pol II TAFs. Our results map the surface of TBP that interacts with Brf and suggest that Pol II and Pol III TAFs interact with the same surface of TBP.

scholarly journals Global Role of TATA Box-Binding Protein Recruitment to Promoters in Mediating Gene Expression Profiles

2004 ◽  
Vol 24 (18) ◽  
pp. 8104-8112 ◽  
Author(s):  
Jonghwan Kim ◽  
Vishwanath R. Iyer
Keyword(s):  
Gene Expression ◽  
Binding Protein ◽  
Core Promoter ◽  
Expression Profiles ◽  
Rna Polymerase Iii ◽  
Gene Expression Profiles ◽  
Tata Box ◽  
Expression Levels ◽  
Tata Box Binding Protein

ABSTRACT The recruitment of TATA box-binding protein (TBP) to promoters is one of the rate-limiting steps during transcription initiation. However, the global importance of TBP recruitment in determining the absolute and changing levels of transcription across the genome is not known. We used a genomic approach to explore the relationship between TBP recruitment to promoters and global gene expression profiles in Saccharomyces cerevisiae. Our data indicate that first, RNA polymerase III promoters are the most prominent binding targets of TBP in vivo. Second, the steady-state transcript levels of genes throughout the genome are proportional to the occupancy of their promoters by TBP, and changes in the expression levels of these genes are closely correlated with changes in TBP recruitment to their promoters. Third, a consensus TATA element does not appear to be a major determinant of either TBP binding or gene expression throughout the genome. Our results indicate that the recruitment of TBP to promoters in vivo is of universal importance in determining gene expression levels in yeast, regardless of the nature of the core promoter or the type of activator or repressor that may mediate changes in transcription. The primary data reported here are available at http://www.iyerlab.org/tbp .

scholarly journals TATA box-binding protein (TBP)-related factor 2 (TRF2), a third member of the TBP family

1999 ◽  
Vol 96 (9) ◽  
pp. 4791-4796 ◽  
Author(s):  
M. D. Rabenstein ◽  
S. Zhou ◽  
J. T. Lis ◽  
R. Tjian
Keyword(s):  
Binding Protein ◽  
Tata Box ◽  
Related Factor ◽  
Tata Box Binding Protein
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