scholarly journals Modeling early stages of endoderm development in epiblast stem cell aggregates with supply of extracellular matrices

2020 ◽  
Vol 62 (4) ◽  
pp. 243-259 ◽  
Author(s):  
Sachiko Inamori ◽  
Mai Fujii ◽  
Sayaka Satake ◽  
Hideaki Iida ◽  
Machiko Teramoto ◽  
...  
Keyword(s):  
Stem Cell ◽  
Extracellular Matrices ◽  
Cell Aggregates ◽  
Early Stages ◽  
Endoderm Development

In vivo study of the angiogenesis potential of bone marrow-derived mesenchymal stem cell aggregates in their niche like environment

2021 ◽  
pp. 039139882110255
Author(s):  
Sara Anajafi ◽  
Azam Ranjbar ◽  
Monireh Torabi-Rahvar ◽  
Naser Ahmadbeigi
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cultured Cells ◽  
Morphological Evidence ◽  
Cell Aggregates ◽  
Plla Scaffold ◽  
Significant Difference

Background: Sufficient blood vessel formation in bioengineered tissues is essential in order to keep the viability of the organs. Impaired development of blood vasculatures results in failure of the implanted tissue. The cellular source which is seeded in the scaffold is one of the crucial factors involved in tissue engineering methods. Materials and methods: Considering the notable competence of Bone Marrow derived Mesenchymal Stem Cell aggregates for tissue engineering purposes, in this study BM-aggregates and expanded BM-MSCs were applied without any inductive agent or co-cultured cells, in order to investigate their own angiogenesis potency in vivo. BM-aggregates and BM-MSC were seeded in Poly-L Lactic acid (PLLA) scaffold and implanted in the peritoneal cavity of mice. Result: Immunohistochemistry results indicated that there was a significant difference ( p < 0.050) in CD31+ cells between PLLA scaffolds contained cultured BM-MSC; PLLA scaffolds contained BM-aggregates and empty PLLA. According to morphological evidence, obvious connections with recipient vasculature and acceptable integration with surroundings were established in MSC and aggregate-seeded scaffolds. Conclusion: Our findings revealed cultured BM-MSC and BM-aggregates, capacity in order to develop numerous connections between PLLA scaffold and recipient’s vasculature which is crucial to the survival of tissues, and considerable tendency to develop constructs containing CD31+ endothelial cells which can contribute in vessel’s tube formation.

scholarly journals Combinatorial Extracellular Matrices for Human Embryonic Stem Cell Differentiation in 3D

2010 ◽  
Vol 11 (8) ◽  
pp. 1909-1914 ◽  
Author(s):  
Fan Yang ◽  
Seung-Woo Cho ◽  
Sun Mi Son ◽  
Sarah P. Hudson ◽  
Said Bogatyrev ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Extracellular Matrices ◽  
Embryonic Stem Cell Differentiation

scholarly journals Plasmonic Nanofactors as Switchable Devices to Promote or Inhibit Neuronal Activity and Function

Nanomaterials ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 1029 ◽  
Author(s):  
Karrer M. Alghazali ◽  
Rabab N. Hamzah ◽  
Zeid A. Nima ◽  
Richard Steiner ◽  
Madhu Dhar ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neuronal Activity ◽  
Tissue Regeneration ◽  
Cellular Proliferation ◽  
Neuronal Cells ◽  
Stem Cell Differentiation ◽  
Extracellular Matrices ◽  
Specific Cancer ◽  
Cancer Cell Targeting ◽  
And Function

Gold nanosystems have been investigated extensively for a variety of applications, from specific cancer cell targeting to tissue regeneration. Specifically, a recent and exciting focus has been the gold nanosystems’ interface with neuronal biology. Researchers are investigating the ability to use these systems neuronal applications ranging from the enhancement of stem cell differentiation and therapy to stimulation or inhibition of neuronal activity. Most of these new areas of research are based on the integration of the plasmonic properties of such nanosystems into complex synthetic extracellular matrices (ECM) that can interact and affect positively the activity of neuronal cells. Therefore, the ability to integrate the plasmonic properties of these nanoparticles into multidimensional and morphological structures to support cellular proliferation and activity is potentially of great interest, particularly to address medical conditions that are currently not fully treatable. This review discusses some of the promising developments and unique capabilities offered by the integration of plasmonic nanosystems into morphologically complex ECM devices, designed to control and study the activity of neuronal cells.

scholarly journals Core–shell patterning of synthetic hydrogels via interfacial bioorthogonal chemistry for spatial control of stem cell behavior

2018 ◽  
Vol 9 (24) ◽  
pp. 5394-5404 ◽  
Author(s):  
K. T. Dicker ◽  
J. Song ◽  
A. C. Moore ◽  
H. Zhang ◽  
Y. Li ◽  
...  
Keyword(s):  
Stem Cell ◽  
Extracellular Matrices ◽  
New Technique ◽  
Cell Behavior ◽  
Core Shell ◽  
Bioorthogonal Chemistry ◽  
Spatial Control ◽  
Guidance Cues ◽  
Synthetic Hydrogels ◽  
A New Technique

A new technique is described for the patterning of cell-guidance cues in synthetic extracellular matrices.

scholarly journals Comprehensive proteomic characterization of stem cell-derived extracellular matrices

Biomaterials ◽  
2017 ◽  
Vol 128 ◽  
pp. 147-159 ◽  
Author(s):  
Héloïse Ragelle ◽  
Alexandra Naba ◽  
Benjamin L. Larson ◽  
Fangheng Zhou ◽  
Miralem Prijić ◽  
...  
Keyword(s):  
Stem Cell ◽  
Extracellular Matrices

Effects of different extracellular matrices and co-cultures on human limbal stem cell expansion in vitro

2009 ◽  
Vol 33 (9) ◽  
pp. 978-987 ◽  
Author(s):  
Naghmeh Ahmadiankia ◽  
Marzieh Ebrahimi ◽  
Ahmad Hosseini ◽  
Hossein Baharvand
Keyword(s):  
Stem Cell ◽  
Cell Expansion ◽  
Extracellular Matrices ◽  
Stem Cell Expansion ◽  
Limbal Stem Cell

scholarly journals Noninvasive sorting of stem cell aggregates based on intrinsic markers

2014 ◽  
Vol 85 (4) ◽  
pp. 353-358 ◽  
Author(s):  
D.G. Buschke ◽  
J.M. Squirrell ◽  
A. Vivekanandan ◽  
C.T. Rueden ◽  
K.W. Eliceiri ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Aggregates
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