Cooperation of Sall4 and Sox8 transcription factors in the regulation of the chicken Sox3
 gene during otic placode development

Yu Okamoto; Naoko Nishimura; Kazunari Matsuda; Deshani C. Ranawakage; Yusuke Kamachi; Hisato Kondoh; Masanori Uchikawa

doi:10.1111/dgd.12427

Cooperation of Sall4 and Sox8 transcription factors in the regulation of the chicken Sox3 gene during otic placode development

Development Growth & Differentiation ◽

10.1111/dgd.12427 ◽

2018 ◽

Vol 60 (3) ◽

pp. 133-145 ◽

Cited By ~ 1

Author(s):

Yu Okamoto ◽

Naoko Nishimura ◽

Kazunari Matsuda ◽

Deshani C. Ranawakage ◽

Yusuke Kamachi ◽

...

Keyword(s):

Transcription Factors ◽

Otic Placode ◽

Sox3 Gene

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PBX1 and MEIS1 up-regulate SOX3 gene expression by direct interaction with a consensus binding site within the basal promoter region

Biochemical Journal ◽

10.1042/bj20090694 ◽

2009 ◽

Vol 425 (1) ◽

pp. 107-116 ◽

Cited By ~ 20

Author(s):

Marija Mojsin ◽

Milena Stevanovic

Keyword(s):

Gene Expression ◽

Transcription Factors ◽

Binding Site ◽

Integration Site ◽

Direct Interaction ◽

Cell Leukaemia ◽

Upstream Stimulatory Factor ◽

Consensus Binding Site ◽

Functional Link ◽

Sox3 Gene

Sox3/SOX3 [SRY (sex determining region Y)-box 3] is considered to be one of the earliest neural markers in vertebrates, playing a role in specifying neuronal fate. We have previously reported characterization of the SOX3 promoter and demonstrated that the general transcription factors NF-Y (nuclear factor-Y), Sp1 (specificity protein 1) and USF (upstream stimulatory factor) are involved in transcriptional regulation of SOX3 promoter activity. In the present study we provide the first evidence that the TALE (three-amino-acid loop extension) transcription factors PBX1 (pre-B-cell leukaemia homeobox 1) and MEIS1 (myeloid ecotropic viral integration site 1 homologue) participate in regulating human SOX3 gene expression in NT2/D1 cells by direct interaction with the consensus PBX/MEIS-binding site, which is conserved in all mammalian orthologue promoters analysed. PBX1 is present in the protein complex formed at this site with nuclear proteins from uninduced cells, whereas both PBX1 and MEIS1 proteins were detected in the complex created with extract from RA (retinoic acid)-induced NT2/D1 cells. By functional analysis we also showed that mutations of the PBX1/MEIS1-binding sites resulted in profound reduction of SOX3 promoter responsiveness to RA. Finally, we demonstrated that overexpressed PBX1 and MEIS1 increased endogenous SOX3 protein expression in both uninduced and RA-induced NT2/D1 cells. With the results of the present study, for the first time, we have established a functional link between the TALE proteins, PBX1 and MEIS1, and expression of the human SOX3 gene. This link is of particular interest since both TALE family members and members of the SOX superfamily are recognized as important developmental regulators.

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Characterization of three novel members of the zebrafish Pax2/5/8 family: dependency of Pax5 and Pax8 expression on the Pax2.1 (noi) function

Development ◽

10.1242/dev.125.16.3063 ◽

1998 ◽

Vol 125 (16) ◽

pp. 3063-3074 ◽

Cited By ~ 31

Author(s):

P.L. Pfeffer ◽

T. Gerster ◽

K. Lun ◽

M. Brand ◽

M. Busslinger

Keyword(s):

Spinal Cord ◽

Transcription Factors ◽

Boundary Region ◽

Optic Stalk ◽

Delayed Onset ◽

Pax8 Expression ◽

Otic Placode ◽

Pax5 Gene ◽

Pax2 Gene

The mammalian Pax2, Pax5 and Pax8 genes code for highly related transcription factors, which play important roles in embryonic development and organogenesis. Here we report the characterization of all members of the zebrafish Pax2/5/8 family. These genes have arisen by duplications before or at the onset of vertebrate evolution. Due to an additional genome amplification in the fish lineage, the zebrafish contains two Pax2 genes, the previously known Pax[b] gene (here renamed as Pax2.1) and a novel Pax2.2 gene. The zebrafish Pax2.1 gene most closely resembles the mammalian Pax2 gene in its expression pattern, as it is transcribed first in the midbrain-hindbrain boundary region, then in the optic stalk, otic system, pronephros and nephric ducts, and lastly in specific interneurons of the hindbrain and spinal cord. Pax2.2 differs from Pax2.1 by the absence of expression in the nephric system and by a delayed onset of transcription in other Pax2.1 expession domains. Pax8 is also expressed in the same domains as Pax2.1, but its transcription is already initiated during gastrulation in the primordia of the otic placode and pronephric anlage, thus identifying Pax8 as the earliest developmental marker of these structures. The zebrafish Pax5 gene, in contrast to its mouse orthologue, is transcribed in the otic system in addition to its prominent expression at the midbrain-hindbrain boundary. The no isthmus (noi) mutation is known to inactivate the Pax2.1 gene, thereby affecting the development of the midbrain-hindbrain boundary region, pronephric system, optic stalk and otic region. Although the different members of the Pax2/5/8 family may potentially compensate for the loss of Pax2.1 function, we demonstrate here that only the expression of the Pax2.2 gene remains unaffected in noi mutant embryos. The expression of Pax5 and Pax8 is either not initiated at the midbrain-hindbrain boundary or is later not maintained in other expression domains. Consequently, the noi mutation of zebrafish is equivalent to combined inactivation of the mouse Pax2 and Pax5 genes with regard to the loss of midbrain-hindbrain boundary development.

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