Cutaneous leishmaniasis: PCR of filter paper blots from an ulcer base is an alternative to biopsy

2018 ◽  
Vol 16 (6) ◽  
pp. 772-774
Author(s):  
Maria Kitchen ◽  
Hanspeter Marti ◽  
Matthias Schmuth
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Filter Paper ◽  
Ulcer Base

PCR performance for the diagnosis of cutaneous leishmaniasis caused by Leishmania viannia complex using biopsy samples, compared with exudate samples from skin lesions on filter paper

2020 ◽  
Vol 114 (10) ◽  
pp. 721-724
Author(s):  
Yahanda Gisela Apaza-Castillo ◽  
Elsa Gladys Aguilar-Ancori ◽  
Mercedes Maritza Quispe-Flórez ◽  
Max Carlos Ramírez-Soto ◽  
Rosa Luz Pacheco-Venero
Keyword(s):  
Skin Lesion ◽  
Diagnostic Accuracy ◽  
Skin Biopsy ◽  
Cutaneous Leishmaniasis ◽  
Filter Paper ◽  
Sampling Methods ◽  
Skin Lesions ◽  
Kappa Coefficient ◽  
Clinical Suspicion ◽  
Diagnostic Concordance

Abstract Background Cutaneous leishmaniasis (CL) is generally diagnosed by molecular methods, including PCR, using biopsy samples, skin scrapings and clinical exudates. In this study, we assessed the PCR performance for diagnosis of CL using skin of biopsy samples vs PCR of skin lesion exudate samples on filter paper and compared the diagnostic concordance of PCR using both sampling methods. Methods We assessed the PCR performance using 80 skin biopsy samples and 80 filter paper samples containing exudates from skin lesions obtained from 74 patients with clinical suspicion of CL in Cusco, Peru. Results : PCR using skin biopsy samples had superior diagnostic accuracy compared with filter paper PCR (62.5% [50/80] vs 38.7% [31/80], respectively; p˂0.005) and the diagnostic concordance between both sampling methods was ‘moderate’ (kappa coefficient=0.50, 95% CI 0.98 to 1.0). Conclusions PCR using biopsy samples remains the standard for diagnosis of CL.

scholarly journals Diagnostic Performance of Filter Paper Lesion Impression PCR for Secondarily Infected Ulcers and Nonulcerative Lesions Caused by Cutaneous Leishmaniasis

2010 ◽  
Vol 49 (3) ◽  
pp. 1097-1100 ◽  
Author(s):  
A. K. Boggild ◽  
A. P. Ramos ◽  
B. M. Valencia ◽  
N. Veland ◽  
F. Calderon ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Filter Paper ◽  
Diagnostic Performance

scholarly journals Detection and Species Identification ofLeishmaniaDNA from Filter Paper Lesion Impressions for Patients with American Cutaneous Leishmaniasis

2010 ◽  
Vol 50 (1) ◽  
pp. e1-e6 ◽  
Author(s):  
Andrea K. Boggild ◽  
Braulio Mark Valencia ◽  
Diego Espinosa ◽  
Nicolas Veland ◽  
Ana Pilar Ramos ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Species Identification ◽  
Filter Paper ◽  
American Cutaneous Leishmaniasis

scholarly journals Filter paper performance in PCR for cutaneous leishmaniasis diagnosis

2021 ◽  
Vol 54 ◽  
Author(s):  
Camila Alves Mota ◽  
Eneide Aparecida Sabaini Venazzi ◽  
Paulo Donizeti Zanzarini ◽  
Sandra Mara Alessi Aristides ◽  
Maria Valdrinez Campana Lonardoni ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Filter Paper

Preservation of Plant Cell Surfaces For Scanning Electron Microscopy

1973 ◽  
Vol 31 ◽  
pp. 472-473
Author(s):  
Linda M. Sicko ◽  
Thomas E. Jensen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Critical Point ◽  
Filter Paper ◽  
Freeze Drying ◽  
Cell Surfaces ◽  
Air Drying ◽  
Popular Method ◽  
Critical Point Drying ◽  
Scanning Electron

The use of critical point drying is rapidly becoming a popular method of preparing biological samples for scanning electron microscopy. The procedure is rapid, and produces consistent results with a variety of samples. The preservation of surface details is much greater than that of air drying, and the procedure is less complicated than that of freeze drying. This paper will present results comparing conventional air-drying of plant specimens to critical point drying, both of fixed and unfixed material. The preservation of delicate structures which are easily damaged in processing and the use of filter paper as a vehicle for drying will be discussed.

SEM Observations on the Germination of Euonymous Americanus

1985 ◽  
Vol 43 ◽  
pp. 508-509
Author(s):  
D.R. Hill ◽  
J.R. McCurry ◽  
L.P. Elliott ◽  
G. Howard
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Filter Paper ◽  
Room Temperature ◽  
Food Source ◽  
Environmental Chamber ◽  
Dormant Stage ◽  
Scanning Electron

Germination of Euonymous americanus in the laboratory has previously been unsuccessful. Ability to germinate Euonymous americanus. commonly known as the american strawberry bush, is important in that it represents a valuable food source for the white-tailed deer. Utilizing the knowledge that its seeds spend a period of time in the rumin fluid of deer during their dormant stage, we were successful in initiating germination. After a three month drying period, the seeds were placed in 25 ml of buffered rumin fluid, pH 8 at 40°C for 48 hrs anaerobically. They were then allowed to dry at room temperature for 24 hrs, placed on moistened filter paper and enclosed within an environmental chamber. Approximately four weeks later germination was detected and verified by scanning electron microscopy; light microscopy provided inadequate resolution. An important point to note in this procedure is that scarification, which was thought to be vital for germination, proved to be unnecessary for successful germination to occur. It is believed that germination was propagated by the secretion of enzymes or prescence of acids produced by microorganisms found in the rumin fluid since sterilized rumin failed to bring about germination.

A freeze-fracture-etched study of the physarum polycephalum plasma membrane during early formation of the macroplasmodial stage

1993 ◽  
Vol 51 ◽  
pp. 346-347
Author(s):  
Randolph W. Taylor ◽  
Henrie Treadwell
Keyword(s):  
Plasma Membrane ◽  
Life Cycle ◽  
Growth Phase ◽  
Filter Paper ◽  
Physarum Polycephalum ◽  
Freeze Fracture ◽  
Petri Dish ◽  
Wire Screen ◽  
Wide Mouth ◽  
Sterile Filter

The plasma membrane of the Slime Mold, Physarum polycephalum, process unique morphological distinctions at different stages of the life cycle. Investigations of the plasma membrane of P. polycephalum, particularly, the arrangements of the intramembranous particles has provided useful information concerning possible changes occurring in higher organisms. In this report Freeze-fracture-etched techniques were used to investigate 3 hours post-fusion of the macroplasmodia stage of the P. polycephalum plasma membrane.Microplasmodia of Physarum polycephalum (M3C), axenically maintained, were collected in mid-expotential growth phase by centrifugation. Aliquots of microplasmodia were spread in 3 cm circles with a wide mouth pipette onto sterile filter paper which was supported on a wire screen contained in a petri dish. The cells were starved for 2 hrs at 24°C. After starvation, the cells were feed semidefined medium supplemented with hemin and incubated at 24°C. Three hours after incubation, samples were collected randomly from the petri plates, placed in plancettes and frozen with a propane-nitrogen jet freezer.

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