scholarly journals Raman spectroscopy for the preoperative diagnosis of thyroid cancer and its subtypes: An in vitro proof-of-concept study

Cytopathology ◽  
2018 ◽  
Vol 30 (1) ◽  
pp. 51-60 ◽  
Author(s):  
Declan O'Dea ◽  
Massimo Bongiovanni ◽  
Gerasimos P. Sykiotis ◽  
Panos G. Ziros ◽  
Aidan D. Meade ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Thyroid Cancer ◽  
Preoperative Diagnosis ◽  
Proof Of Concept ◽  
Concept Study ◽  
Diagnosis Of Thyroid Cancer

X-RAY FLUORESCENCE ANALYSIS IN THE DIAGNOSIS OF THYROID CANCER

2017 ◽  
pp. 39-45
Author(s):  
В. Зайчик ◽  
V. Zaychik ◽  
Г. Давыдов ◽  
G. Davydov
Keyword(s):  
Thyroid Cancer ◽  
Normal Tissue ◽  
Thyroid Tissue ◽  
Nodular Goiter ◽  
Fluorescence Analysis ◽  
Fluorescence Excitation ◽  
X Ray ◽  
Thyroid Goiter ◽  
Diagnosis Of Thyroid Cancer

Purpose: To investigate new possibilities of differential diagnosis of benign and malignant thyroid goiter lesions by means of energy dispersive X-ray fluorescence analysis (EDRFA). Material and methods: In the samples of thyroid tissue taken from people with intact thyroid gland (mostly died from trauma, n = 92), as well as in patients with nodular goiter (n = 79) and thyroid cancer (n = 40) bromine (Br), copper (Cu), iron (Fe), iodine (I), rubidium (Rb) and strontium (Sr) were investigated. To determine these elements, the methods of EDRPA have been developed using encapsulated sources with 109Cd and 241Am radionuclides for fluorescence excitation. Results: It is shown that the levels of the content of I, the ratios I / Cu and I / Rb, as well as the products of the ratios I / Cu ∙ (I / Rb) and (I / Br) ∙ (I / Cu) ∙ (I / Rb ) are highly informative markers of thyroid cancer. The accuracy of the developed methods and the reliability of the results obtained were confirmed by measurements of international certified comparison materials. Conclusion: The use of the proposed markers allows in vitro to differentiat thyroid cancer from benign nodes and normal tissue with sensitivity in the range of 86–100 %, specificity of 89–99 %, and accuracy within 90–99 %.

In vitro effects of mechanical stimulation and photobiomodulation on osteoblastic cell function: A proof of concept study

2017 ◽  
Vol 27 (1) ◽  
pp. 29-41 ◽  
Author(s):  
Rochaya Chintavalakorn ◽  
Anak Khantachawana ◽  
Kwanchanok Viravaidya-Pasuwat ◽  
Peerapong Santiwong ◽  
Rudee Surarit
Keyword(s):  
Mechanical Stimulation ◽  
Cell Function ◽  
Osteoblastic Cell ◽  
Proof Of Concept ◽  
Concept Study ◽  
In Vitro Effects

scholarly journals Microvalve bioprinting of MSC-Chondrocyte Co-Cultures

2021 ◽  
Author(s):  
Joseph Dudman ◽  
Ana Marina Ferreira ◽  
Piergiorgio Gentile ◽  
Xiao Wang ◽  
Kenneth Dalgarno
Keyword(s):  
High Throughput Screening ◽  
Cellular Therapy ◽  
Cell Types ◽  
Inhibitory Effect ◽  
Proof Of Concept ◽  
Concept Study ◽  
Indirect Immunofluorescence Staining ◽  
Chondrocyte Implantation ◽  
Tissue Models

Recent improvements within the fields of high-throughput screening and 3D tissue culture have provided the possibility of developing in vitro micro-tissue models that can be used to study diseases and screen potential new therapies. This paper reports a proof of concept study on the use of microvalve-based bioprinting to create laminar MSC-chondrocyte co-cultures as an in vitro model of autologous chondrocyte implantation (ACI), an established cellular therapy for osteoarthritis. Microvalve-based bioprinting uses microvalves to deposit cells suspended in a liquid in a consistent and repeatable manner. In this case MSCs and chondrocytes have been sequentially deposited into an insert based transwell system in order to create a laminar co-culture, with variations in the ratios of the cell types used to investigate the potential for MSCs to stimulate improved repair. Histological and indirect immunofluorescence staining revealed the formation of dense tissue structures within the chondrocyte and MSC-chondrocyte cell co-cultures, alongside the establishment of a proliferative region at the base of the tissue. No stimulatory or inhibitory effect in terms of ECM production was observed through the introduction of MSCs, although the potential for an immunomodulatory benefit remains. This proof-of-concept study therefore provides a novel method to enable the scalable production of therapeutically relevant micro-tissue models that can be used for in vitro research to optimise ACI procedures.

Serous BMP8A has Clinical Significance in the Ultrasonic Diagnosis of Thyroid Cancer and Promotes Thyroid Cancer Cell Progression

2020 ◽  
Vol 20 (4) ◽  
pp. 591-598 ◽  
Author(s):  
Kun Liu ◽  
Min Gao ◽  
Dongdong Qin ◽  
Hongjun Wang ◽  
Qixiu Lu
Keyword(s):  
Thyroid Cancer ◽  
Early Diagnosis ◽  
Cell Viability ◽  
Soft Agar ◽  
The Cancer Genome Atlas ◽  
Anchorage Independent Growth ◽  
Over Expression ◽  
Diagnosis Of Thyroid Cancer

Objectives: This study aims to discover a potential cytokine biomarker for early diagnosis of thyroid cancer. Methods: We employed data mining of The Cancer Genome Atlas (TCGA) and experimentally elucidated its mechanistic contributions. The differential expression genes (DEGs) between thyroid cancer and health population were analyzed with TCGA online bioinformatic tools. The relative expression of Bone Morphogenetic Protein 8A (BMP8A) was determined by real-time PCR in ultrasonic diagnosed thyroid cancer both in vivo and in vitro. The serous BMP8A content was quantified with an ELISA kit. Protein levels of BMP8A, OCLN, ZEB1, EZH2 and β-Actin were analyzed by Western blot. Cell viability was measured by the MTT assay, and anchorage-independent growth was measured by the soft agar colony formation assay. Cell migrative and invasive capacities were interrogated with transwell chamber assays. Results: We identified aberrantly high expression of BMP8A in thyroid cancer, which was associated with unfavorable prognosis and tumor progression. The serous BMP8A was also significantly up-regulated in thyroid cancer patients. Ectopic over-expression of BMP8A remarkably stimulated cell viability and anchorage-independent growth. Meanwhile, the migrative and invasive capacities were greatly increased in response to BMP8A over-expression. Mechanistically, we characterized the positive correlation between BMP8A and TCF7L1, and forced expression of TCF7L1 induced BMP8A expression in TPC-1 cells. Conclusion: In summary, we have identified a novel biomarker for early diagnosis in addition to Ultrasound for thyroid cancer, which is subjected to TCF7L1 regulation.

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