Cytoplasmic expression of PMS2 (clone EP51 ), PRAME , and STAT6 (clone YE361 ) as a potential immunohistochemical finding for detection of sebocyte differentiation

2021 ◽  
Author(s):  
Keisuke Goto ◽  
Takashi Sugino
Keyword(s):  
Cytoplasmic Expression ◽  
Immunohistochemical Finding

scholarly journals Intracellular Routing and Recognition of Lipid-Based mRNA Nanoparticles

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 945
Author(s):  
Christophe Delehedde ◽  
Luc Even ◽  
Patrick Midoux ◽  
Chantal Pichon ◽  
Federico Perche
Keyword(s):  
Gene Therapy ◽  
Immune Responses ◽  
Transient Expression ◽  
Messenger Rna ◽  
Lipid Nanoparticles ◽  
Delivery Systems ◽  
Clinical Applications ◽  
Cellular Proteins ◽  
Mrna Sequence ◽  
Cytoplasmic Expression

Messenger RNA (mRNA) is being extensively used in gene therapy and vaccination due to its safety over DNA, in the following ways: its lack of integration risk, cytoplasmic expression, and transient expression compatible with fine regulations. However, clinical applications of mRNA are limited by its fast degradation by nucleases, and the activation of detrimental immune responses. Advances in mRNA applications, with the recent approval of COVID-19 vaccines, were fueled by optimization of the mRNA sequence and the development of mRNA delivery systems. Although delivery systems and mRNA sequence optimization have been abundantly reviewed, understanding of the intracellular processing of mRNA is mandatory to improve its applications. We will focus on lipid nanoparticles (LNPs) as they are the most advanced nanocarriers for the delivery of mRNA. Here, we will review how mRNA therapeutic potency can be affected by its interactions with cellular proteins and intracellular distribution.

Endocrine pancreatic tissue plasticity in obese humans is associated with cytoplasmic expression of PBX-1 in pancreatic ductal cells

2005 ◽  
Vol 333 (4) ◽  
pp. 1153-1159 ◽  
Author(s):  
Ghaffar Muharram ◽  
Anthony Beucher ◽  
Ericka Moerman ◽  
Sandrine Belaı¨ch ◽  
Valéry Gmyr ◽  
...  
Keyword(s):  
Pancreatic Tissue ◽  
Cytoplasmic Expression ◽  
Ductal Cells ◽  
Pancreatic Ductal Cells

High cytoplasmic expression of p27Kip1 is associated with a worse cancer-specific survival in clear cell renal cell carcinoma

2011 ◽  
Vol 109 (10) ◽  
pp. 1565-1570 ◽  
Author(s):  
Stephan Kruck ◽  
Axel S. Merseburger ◽  
Joerg Hennenlotter ◽  
Marcus Scharpf ◽  
Christian Eyrich ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Clear Cell ◽  
Cell Renal Cell Carcinoma ◽  
Cancer Specific Survival ◽  
Cytoplasmic Expression

scholarly journals RU5 of Mason-Pfizer Monkey Virus 5′ Long Terminal Repeat Enhances Cytoplasmic Expression of Human Immunodeficiency Virus Type 1 gag-pol and Nonviral Reporter RNA

2002 ◽  
Vol 76 (20) ◽  
pp. 10211-10218 ◽  
Author(s):  
Stacey Hull ◽  
Kathleen Boris-Lawrie
Keyword(s):  
Long Terminal Repeat ◽  
Profile Analysis ◽  
Foamy Virus ◽  
Terminal Repeat ◽  
Posttranscriptional Control ◽  
Gag Protein ◽  
Cytoplasmic Expression ◽  
Virus Rna ◽  
Intron Removal ◽  
Responsive Element

ABSTRACT Retroviruses utilize an unspliced version of their primary transcription product as an RNA template for synthesis of viral Gag and Pol structural and enzymatic proteins. Cytoplasmic expression of the gag-pol RNA is achieved despite the lack of intron removal and the presence of a long and highly structured 5′ untranslated region that inhibits efficient ribosome scanning. In this study, we have identified for the first time that the 5′ long terminal repeat (LTR) of Mason-Pfizer monkey virus (MPMV) facilitates Rev/Rev-responsive element-independent expression of HIV-1 gag-pol reporter RNA. The MPMV RU5 region of the LTR is necessary and directs functional interaction with cellular posttranscriptional modulators present in human 293 and monkey COS cells but not in quail QT-6 cells and does not require any viral protein. Deletion of MPMV RU5 decreases the abundance of spliced mRNA but has little effect on cytoplasmic accumulation of unspliced gag-pol RNA despite complete elimination of detectable Gag protein production. MPMV RU5 also exerts a positive effect on the cytoplasmic expression of intronless luc RNA, and ribosomal profile analysis demonstrates that MPMV RU5 directs subcellular localization of the luc transcript to polyribosomes. Our findings have a number of similarities with those of reports on 5′ terminal posttranscriptional control elements in spleen necrosis virus and human foamy virus RNA and support the model that divergent retroviruses share 5′ terminal RNA elements that interact with host proteins to program retroviral RNA for productive cytoplasmic expression.

Comparison of immunohistochemical profiles of ovarian germ cells in dysgerminomas of a captive maned wolf and domestic dogs

2021 ◽  
pp. 104063872110199
Author(s):  
Rafael B. Rosa ◽  
Matheus V. Bianchi ◽  
Paula R. Ribeiro ◽  
Fernando F. Argenta ◽  
Andréia Vielmo ◽  
...  
Keyword(s):  
Germ Cells ◽  
Poor Prognosis ◽  
Expression Profiles ◽  
Clinical Signs ◽  
Domestic Dogs ◽  
Domestic Dog ◽  
Placental Alkaline Phosphatase ◽  
Immunohistochemical Expression ◽  
Cytoplasmic Expression ◽  
Maned Wolf

We characterized the immunohistochemical expression profiles of dysgerminomas from a 16-y-old maned wolf and 13 domestic dogs using the following biomarkers: Sal-like protein 4 (SALL4), octamer-binding transcription factor 3/4 (OCT3/4), placental alkaline phosphatase (PLAP), c-kit, and vimentin. The maned wolf had nonspecific and long-standing clinical signs of lethargy, anorexia, and weight loss, and was euthanized because of poor prognosis. At autopsy, the left ovary was effaced by a 12 × 8 × 6 cm mass, comprised of anaplastic cells with a mitotic count of 20 mitoses in 10 high power fields. Dysgerminomas from 7 of 13 domestic dogs had nuclear expression of SALL4. Dysgerminomas from the maned wolf and 2 domestic dogs had both nuclear and cytoplasmic expression of SALL4. Cytoplasmic expression of PLAP and OCT3/4 was present in dysgerminomas from the maned wolf and 3 (PLAP) or 4 (OCT3/4) domestic dogs. All dysgerminomas expressed vimentin. Membranous c-kit expression was rare in the dysgerminoma from the maned wolf, and variable in dysgerminomas from 4 domestic dogs. A dysgerminoma from a domestic dog had cytoplasmic expression of c-kit. SALL4 is a useful marker to confirm germ cell origin of dysgerminoma in canids.

The cytoplasmic expression of E-cadherin and β-catenin in bovine trophoblasts during binucleate cell differentiation

Placenta ◽  
2005 ◽  
Vol 26 (5) ◽  
pp. 393-401 ◽  
Author(s):  
H. Nakano ◽  
A. Shimada ◽  
K. Imai ◽  
T. Takahashi ◽  
K. Hashizume
Keyword(s):  
Cell Differentiation ◽  
Binucleate Cell ◽  
Cytoplasmic Expression ◽  
E Cadherin

P-613 Cytoplasmic expression of Cyclin D1 correlates with tumorrecurrence and mortality in stage I NSCLC

Lung Cancer ◽  
2005 ◽  
Vol 49 ◽  
pp. S279-S280
Author(s):  
A. Gajra ◽  
N. Vajpayee ◽  
M. Wade ◽  
O. El-Zamar ◽  
E. Dexter ◽  
...  
Keyword(s):  
Cyclin D1 ◽  
Stage I ◽  
Cytoplasmic Expression
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