scholarly journals Requirments for primary human hepatocyte

2021 ◽  
Author(s):  
Zhaoliang Peng ◽  
Jiaying Wu ◽  
Shijun Hu ◽  
Aijin Ma ◽  
Lei Wang ◽  
...  
Keyword(s):  
Human Hepatocyte ◽  
Primary Human Hepatocyte

Elucidating Extracellular Matrix and Stiffness Control of Primary Human Hepatocyte Phenotype via Cell Microarrays

2021 ◽  
pp. 2101284
Author(s):  
Chase P. Monckton ◽  
Aidan Brougham‐Cook ◽  
Kerim B. Kaylan ◽  
Gregory H. Underhill ◽  
Salman R. Khetani
Keyword(s):  
Extracellular Matrix ◽  
Human Hepatocyte ◽  
Primary Human Hepatocyte ◽  
Cell Microarrays ◽  
Stiffness Control

scholarly journals Assessing the compatibility of primary human hepatocyte culture within porous silk sponges

RSC Advances ◽  
2020 ◽  
Vol 10 (62) ◽  
pp. 37662-37674
Author(s):  
David A. Kukla ◽  
Whitney L. Stoppel ◽  
David L. Kaplan ◽  
Salman R. Khetani
Keyword(s):  
Extracellular Matrix ◽  
Extracellular Matrix Proteins ◽  
Human Hepatocytes ◽  
Human Hepatocyte ◽  
Matrix Proteins ◽  
Hepatocyte Culture ◽  
Primary Human Hepatocytes ◽  
Primary Human Hepatocyte ◽  
Silk Scaffolds ◽  
Parenchymal Cells

Porous silk scaffolds hybridized with extracellular matrix proteins are useful for culture of primary human hepatocytes ± supportive non-parenchymal cells.

scholarly journals Intermittent Starvation Extends the Functional Lifetime of Primary Human Hepatocyte Cultures

2020 ◽  
Vol 174 (2) ◽  
pp. 266-277
Author(s):  
Matthew D Davidson ◽  
Salman R Khetani
Keyword(s):  
Drug Exposure ◽  
Adenosine Monophosphate ◽  
Human Hepatocyte ◽  
Toxicity Tests ◽  
Drug Induced ◽  
Nonparenchymal Cells ◽  
Primary Human Hepatocyte ◽  
Serum Hormone

Abstract Primary human hepatocyte (PHH) cultures have become indispensable to mitigate the risk of adverse drug reactions in human patients. In contrast to dedifferentiating monocultures, coculture with nonparenchymal cells maintains PHH functions for 2–4 weeks. However, because the functional lifespan of PHHs in vivo is 200–400 days, it is desirable to further prolong PHH functions in vitro toward modeling chronic drug exposure and disease progression. Fasting has benefits on the longevity of organisms and the health of tissues such as the liver. We hypothesized that a culturing protocol that mimics dynamic fasting/starvation could activate starvation pathways and prolong PHH functional lifetime. To mimic starvation, serum and hormones were intermittently removed from the culture medium of micropatterned cocultures (MPCCs) containing PHHs organized onto collagen domains and surrounded by 3T3-J2 murine fibroblasts. A weekly 2-day starvation optimally prolonged PHH functional lifetime for 6+ weeks in MPCCs versus a decline after 3 weeks in nonstarved controls. The 2-day starvation also enhanced the functions of PHH monocultures for 2 weeks, suggesting direct effects on PHHs. In MPCCs, starvation activated 5' adenosine monophosphate-activated protein kinase (AMPK) and restricted fibroblast overgrowth onto PHH islands, thereby maintaining hepatic polarity. The effects of starvation on MPCCs were partially recapitulated by activating AMPK using metformin or growth arresting fibroblasts via mitomycin-C. Lastly, starved MPCCs demonstrated lower false positives for drug toxicity tests and higher drug-induced cytochrome-P450 activities versus nonstarved controls even after 5 weeks. In conclusion, intermittent serum/hormone starvation extends PHH functional lifetime toward enabling clinically relevant drug screening.

scholarly journals Mechanistic evaluation of primary human hepatocyte culture using global proteomic analysis reveals a selective dedifferentiation profile

2016 ◽  
Vol 91 (1) ◽  
pp. 439-452 ◽  
Author(s):  
James A. Heslop ◽  
Cliff Rowe ◽  
Joanne Walsh ◽  
Rowena Sison-Young ◽  
Roz Jenkins ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Human Hepatocyte ◽  
Hepatocyte Culture ◽  
Primary Human Hepatocyte

In vitro primary human hepatocyte 3D spheroid model for hepatotoxicity studies

2019 ◽  
Vol 34 (1) ◽  
pp. S36
Author(s):  
Feng Li ◽  
Sweta Parikh ◽  
Li Cao ◽  
Kirsten Cooper ◽  
Rongjun Zuo
Keyword(s):  
Human Hepatocyte ◽  
Primary Human Hepatocyte

Reexpression of cytokeratin-19 in a primary human hepatocyte culture

1998 ◽  
Vol 126 (1) ◽  
pp. 748-750
Author(s):  
A. P. Kiyasov ◽  
A. A. Gumerova ◽  
S. V. Petrov ◽  
S. H. Yap
Keyword(s):  
Human Hepatocyte ◽  
Hepatocyte Culture ◽  
Cytokeratin 19 ◽  
Primary Human Hepatocyte

scholarly journals In Vitro Model for Hepatotoxicity Studies Based on Primary Human Hepatocyte Cultivation in a Perfused 3D Bioreactor System

2016 ◽  
Vol 17 (4) ◽  
pp. 584 ◽  
Author(s):  
Fanny Knöspel ◽  
Frank Jacobs ◽  
Nora Freyer ◽  
Georg Damm ◽  
An De Bondt ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Human Hepatocyte ◽  
Primary Human Hepatocyte ◽  
Bioreactor System ◽  
Vitro Model
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