scholarly journals Dynamic formation of cellular aggregates of chondrocytes and mesenchymal stem cells in spinner flask

2019 ◽  
Vol 52 (4) ◽  
Author(s):  
Huimin He ◽  
Qing He ◽  
Feiyue Xu ◽  
Yan Zhou ◽  
Zhaoyang Ye ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Spinner Flask ◽  
Cellular Aggregates ◽  
Dynamic Formation

scholarly journals Agitation in a microcarrier-based spinner flask bioreactor modulates homeostasis of human mesenchymal stem cells

2021 ◽  
Vol 168 ◽  
pp. 107947
Author(s):  
Richard Jeske ◽  
Shaquille Lewis ◽  
Ang-Chen Tsai ◽  
Kevin Sanders ◽  
Chang Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Spinner Flask

scholarly journals Large-Scale Expansion of Human Mesenchymal Stem Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-17 ◽  
Author(s):  
Muhammad Najib Fathi Bin Hassan ◽  
Muhammad Dain Yazid ◽  
Mohd Heikal Mohd Yunus ◽  
Shiplu Roy Chowdhury ◽  
Yogeswaran Lokanathan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Large Scale ◽  
Human Mesenchymal Stem Cells ◽  
Expansion Ratio ◽  
Spinner Flask ◽  
Roller Bottle ◽  
Multipotent Stem Cells ◽  
Cardiovascular Damage ◽  
Scale Expansion

Mesenchymal stem cells (MSCs) are multipotent stem cells with strong immunosuppressive property that renders them an attractive source of cells for cell therapy. MSCs have been studied in multiple clinical trials to treat liver diseases, peripheral nerve damage, graft-versus-host disease, autoimmune diseases, diabetes mellitus, and cardiovascular damage. Millions to hundred millions of MSCs are required per patient depending on the disease, route of administration, frequency of administration, and patient body weight. Multiple large-scale cell expansion strategies have been described in the literature to fetch the cell quantity required for the therapy. In this review, bioprocessing strategies for large-scale expansion of MSCs were systematically reviewed and discussed. The literature search in Medline and Scopus databases identified 26 articles that met the inclusion criteria and were included in this review. These articles described the large-scale expansion of 7 different sources of MSCs using 4 different bioprocessing strategies, i.e., bioreactor, spinner flask, roller bottle, and multilayered flask. The bioreactor, spinner flask, and multilayered flask were more commonly used to upscale the MSCs compared to the roller bottle. Generally, a higher expansion ratio was achieved with the bioreactor and multilayered flask. Importantly, regardless of the bioprocessing strategies, the expanded MSCs were able to maintain its phenotype and potency. In summary, the bioreactor, spinner flask, roller bottle, and multilayered flask can be used for large-scale expansion of MSCs without compromising the cell quality.

scholarly journals Microencapsulation of cellular aggregate composed of differentiated insulin and glucagon-producing cells from human mesenchymal stem cells derived from adipose tissue.

2020 ◽  
Author(s):  
Claudia Jara ◽  
Felipe Oyarzun-Ampuero ◽  
Flavio Carrión ◽  
Esteban González-Echeverría ◽  
Claudio Cappelli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Glycemic Control ◽  
Sodium Alginate ◽  
Type I Diabetes ◽  
Human Mesenchymal Stem Cells ◽  
Autoimmune Response ◽  
Type I ◽  
Cellular Aggregates

Abstract Background. In type I diabetes mellitus (T1DM) pancreatic β cells are destroyed. Treatment entails exogenous insulin administration and strict diet control, yet optimal glycemic control is hardly attainable. Islet transplant could be an alternative in patients with poor glycemic control, but inefficient islet purification and autoimmune response of patients still a challenge. Methods Human adipose-derived mesenchymal stem cells (hASC) obtained from lipoaspirated fat tissue from human donors were differentiated in vitro to insulin (Ins) and glucagon (Gcg) producing cells (IPC and GPC respectively). Then, we cocultured IPC and GPC cells in low adhesion conditions to form cellular aggregates, which were encapsulated in a sodium alginate polymer. Expression of pancreatic lineage markers and secretion of insulin or glucagon in vitro were analyzed. Results We demonstrated that multipotent hASC efficiently differentiate to IPC and GPC, which also express pancreatic markers, including insulin or glucagon hormones. In turn, we calculated the Feret diameter of cellular aggregates, finding mean diameters ~80 µm at 72h of incubation. IPC/GPC aggregates were then microencapsulated in sodium-alginate polymer microgels, which were found to be more stable in Ba 2+ stabilized microgels, with average diameters ~300 µm. Interestingly, Ba 2+ -microencapsulated aggregates respond to high external glucose with insulin secretion. Conclusions The IPC/GPC differentiation process from hASC followed by generate cellular aggregates in vitro, that once microencapsulated could represent a possible treatment to T1DM.

scholarly journals The Effects of Different Dynamic Culture Systems on Cell Proliferation and Osteogenic Differentiation in Human Mesenchymal Stem Cells

2019 ◽  
Vol 20 (16) ◽  
pp. 4024
Author(s):  
Hsiou-Hsin Tsai ◽  
Kai-Chiang Yang ◽  
Meng-Huang Wu ◽  
Jung-Chih Chen ◽  
Ching-Li Tseng
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Flow Reactor ◽  
Human Mesenchymal Stem Cells ◽  
Spinner Flask ◽  
Dynamic Culture ◽  
Bidirectional Flow

The culture environment plays an important role for stem cells’ cultivation. Static or dynamic culture preserve differential potentials to affect human mesenchymal stem cells’ (hMSCs) proliferation and differentiation. In this study, hMSCs were seeded on fiber disks and cultured in a bidirectional-flow bioreactor or spinner-flask bioreactor with a supplement of osteogenic medium. The hMSCs’ proliferation, osteogenic differentiation, and extracellular matrix deposition of mineralization were demonstrated. The results showed that the spinner flask improved cell viability at the first two weeks while the bidirectional-flow reactor increased the cell proliferation of hMSCs through the four-week culture period. Despite the flow reactor having a higher cell number, a lower lactose/glucose ratio was noted, revealing that the bidirectional-flow bioreactor provides better oxygen accessibility to the cultured cells/disk construct. The changes of calcium ions in the medium, the depositions of Ca2+ in the cells/disk constructs, and alkaline phosphate/osteocalcin activities showed the static culture of hMSCs caused cells to mineralize faster than the other two bioreactors but without cell proliferation. Otherwise, cells were distributed uniformly with abundant extracellular matrix productions using the flow reactor. This reveals that the static and dynamic cultivations regulated the osteogenic process differently in hMSCs. The bidirectional-flow bioreactor can be used in the mass production and cultivation of hMSCs for applications in bone regenerative medicine.

Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm

2010 ◽  
Vol 30 (6) ◽  
pp. 455-455 ◽  
Author(s):  
Dongyan Shi ◽  
Dan Ma ◽  
Feiqing Dong ◽  
Chen Zong ◽  
Liyue Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells

1417: Mesenchymal Stem Cells Alone or Modified with ENOS Restore Erectile Function in the Aged Rat: Cell-Based Gene Therapy for Erectile Dysfunction

2004 ◽  
Vol 171 (4S) ◽  
pp. 373-373
Author(s):  
Trinity J. Bivalacqua ◽  
Mustafa F. Usta ◽  
Hunter C. Champion ◽  
Weiwen Deng ◽  
Philip J. Kadowitz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Mesenchymal Stem Cells ◽  
Erectile Dysfunction ◽  
Erectile Function ◽  
Aged Rat

Placenta mesenchymal stem cells on a chorion scaffold as a potential osteogenic graft for peripartal bone regeneration

2008 ◽  
Vol 68 (S 01) ◽  
Author(s):  
S Mohr ◽  
BC Portmann-Lanz ◽  
A Schoeberlein ◽  
R Sager ◽  
DV Surbek
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration
Sign in / Sign up

Export Citation Format

Share Document