scholarly journals Demethylation of SFRP2 by histone demethylase KDM2A regulated osteo-/dentinogenic differentiation of stem cells of the apical papilla

2016 ◽  
Vol 49 (3) ◽  
pp. 330-340 ◽  
Author(s):  
Guoxia Yu ◽  
Jinsong Wang ◽  
Xiao Lin ◽  
Shu Diao ◽  
Yu Cao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Histone Demethylase ◽  
Apical Papilla ◽  
Dentinogenic Differentiation

scholarly journals KDM1A regulated the osteo/dentinogenic differentiation process of the stem cells of the apical papilla via binding with PLOD2

2018 ◽  
Vol 51 (4) ◽  
pp. e12459 ◽  
Author(s):  
Lijun Wang ◽  
Haoqing Yang ◽  
Xiao Lin ◽  
Yangyang Cao ◽  
Peipei Gao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Differentiation Process ◽  
Apical Papilla ◽  
Dentinogenic Differentiation

scholarly journals Depletion of SNRNP200 inhibits the osteo−/dentinogenic differentiation and cell proliferation potential of stem cells from the apical papilla

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Xiaomin Su ◽  
Haoqing Yang ◽  
Ruitang Shi ◽  
Chen Zhang ◽  
Huina Liu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Cell Proliferation ◽  
S Phase ◽  
Alizarin Red ◽  
Differentiation Potential ◽  
Alp Activity ◽  
Proliferation And Differentiation ◽  
Apical Papilla ◽  
Dentinogenic Differentiation

Abstract Background Tissue regeneration mediated by mesenchymal stem cells (MSCs) is deemed a desirable way to repair teeth and craniomaxillofacial tissue defects. Nevertheless, the molecular mechanisms about cell proliferation and committed differentiation of MSCs remain obscure. Previous researches have proved that lysine demethylase 2A (KDM2A) performed significant function in the regulation of MSC proliferation and differentiation. SNRNP200, as a co-binding factor of KDM2A, its potential effect in regulating MSCs’ function is still unclear. Therefore, stem cells from the apical papilla (SCAPs) were used to investigate the function of SNRNP200 in this research. Methods The alkaline phosphatase (ALP) activity assay, Alizarin Red staining, and osteogenesis-related gene expressions were used to examine osteo−/dentinogenic differentiation potential. Carboxyfluorescein diacetate, succinimidyl ester (CFSE) and cell cycle analysis were applied to detect the cell proliferation. Western blot analysis was used to evaluate the expressions of cell cycle-related proteins. Results Depletion of SNRNP200 caused an obvious decrease of ALP activity, mineralization formation and the expressions of osteo−/dentinogenic genes including RUNX2, DSPP, DMP1 and BSP. Meanwhile, CFSE and cell cycle assays revealed that knock-down of SNRNP200 inhibited the cell proliferation and blocked cell cycle at the G2/M and S phase in SCAPs. In addition, it was found that depletion of SNRNP200 up-regulated p21 and p53, and down-regulated the CDK1, CyclinB, CyclinE and CDK2. Conclusions Depletion of SNRNP200 repressed osteo−/dentinogenic differentiation potentials and restrained cell proliferation through blocking cell cycle progression at the G2/M and S phase, further revealing that SNRNP200 has crucial effects on preserving the proliferation and differentiation potentials of dental tissue-derived MSCs.

scholarly journals The Histone Demethylase KDM3B Promotes Osteo-/Odontogenic Differentiation, Cell Proliferation, and Migration Potential of Stem Cells from the Apical Papilla

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Chen Zhang ◽  
Xiao Han ◽  
Yuncun Liang ◽  
Huina Liu ◽  
Zhipeng Fan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Histone Demethylase ◽  
Alp Activity ◽  
Cell Proliferation And Migration ◽  
Odontogenic Differentiation ◽  
Migration Potential ◽  
Apical Papilla ◽  
And Migration ◽  
Proliferation And Migration

Understanding the regulation mechanisms of mesenchymal stem cells (MSCs) can assist in tissue regeneration. The histone demethylase (KDM) family has a crucial role in differentiation and cell proliferation of MSCs, while the function of KDM3B in MSCs is not well understood. In this study, we used the stem cells from the apical papilla (SCAPs) to test whether KDM3B could regulate the function of MSCs. By an alkaline phosphatase (ALP) activity assay, Alizarin red staining, real-time RT-PCR, and western blot analysis, we found that KDM3B enhanced the ALP activity and mineralization of SCAPs and promoted the expression of runt-related transcription factor 2 (RUNX2), osterix (OSX), dentin sialophosphoprotein (DSPP), and osteocalcin (OCN). Additionally, the CFSE, CCK-8, and flow cytometry assays revealed that KDM3B improved cell proliferation by accelerating cell cycle transition from the G1 to S phase. Scratch and transwell migration assays displayed that KDM3B promoted the migration potential of SCAPs. Mechanically, microarray results displayed that 98 genes were upregulated, including STAT1, CCND1, and FGF5, and 48 genes were downregulated after KDM3B overexpression. Besides, we found that the Toll-like receptor and JAK-STAT signaling pathway may be involved in the regulating function of KDM3B in SCAPs. In brief, we discovered that KDM3B promoted the osteo-/odontogenic differentiation, cell proliferation, and migration potential of SCAPs and provided a novel target and theoretical basis for regenerative medicine.

scholarly journals LBH589 Promotes Osteogenic and Dentinogenic Differentiation of Stem Cells from the Apical Papilla by Inhibiting Histone Deacetylation

2014 ◽  
Vol 23 (3) ◽  
pp. 335-342
Author(s):  
Dayong Liu ◽  
Yuejun Wang ◽  
Chunyue Feng ◽  
Ting Cui ◽  
Mengming Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Histone Deacetylation ◽  
Apical Papilla ◽  
Dentinogenic Differentiation
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