scholarly journals Histone modifications interact with DNA methylation at the GATA4 promoter during differentiation of mesenchymal stem cells into cardiomyocyte-like cells

2016 ◽  
Vol 49 (3) ◽  
pp. 315-329 ◽  
Author(s):  
Hao Xu ◽  
Qin Yi ◽  
Chunmei Yang ◽  
Yue Wang ◽  
Jie Tian ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Histone Modifications

scholarly journals Genome-Wide DNA Methylation Analysis during Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Yangyang Cao ◽  
Haoqing Yang ◽  
Luyuan Jin ◽  
Juan Du ◽  
Zhipeng Fan
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Biological Process ◽  
Cpg Methylation ◽  
Genome Wide ◽  
Marrow Mesenchymal Stem Cells ◽  
Go Terms

Bone marrow mesenchymal stem cells (BMSCs) nowadays are regarded as promising candidates in cell-based therapy for the regeneration of damaged bone tissues that are either incurable or intractable due to the insufficiency of current therapies. Recent studies suggest that BMSCs differentiate into osteoblasts, and that this differentiation is regulated by some specific patterns of epigenetic modifications, such as DNA methylation. However, the potential role of DNA methylation modification in BMSC osteogenic differentiation is unclear. In this study, we performed a genome-wide study of DNA methylation between the noninduced and induced osteogenic differentiation of BMSCs at day 7. We found that the majority of cytosines in a CpG context were methylated in induced BMSCs. Our results also revealed that, along with the induced osteogenic differentiation in BMSCs, the average genomic methylation levels and CpG methylation in transcriptional factor regions (TFs) were increased, the CpG methylation level of various genomic elements was mainly in the medium-high methylation section, and CpG methylation levels in the repeat element had highly methylated levels. The GO analysis of differentially methylated region- (DMR-) associated genes (DMGs) showed that GO terms, including cytoskeletal protein binding (included in Molecular Function GO terms), skeletal development (included in Biological Process GO terms), mesenchymal cell differentiation (included in Biological Process GO terms), and stem cell differentiation (included in Biological Process), were enriched in the hypermethylated DMGs. Then, the KEGG analysis results showed that the WNT pathway, inositol phosphate metabolism pathway, and cocaine addiction pathway were more correlative with the DMRs during the induced osteogenic differentiation in BMSCs. In conclusion, this study revealed the difference of methylated levels during the noninduced and induced osteogenic differentiation of BMSCs and provided useful information for future works to characterize the important function of epigenetic mechanisms on BMSCs’ differentiation.

Genome‐wide DNA‐methylation profiles in human bone marrow mesenchymal stem cells on titanium surfaces

2019 ◽  
Vol 127 (3) ◽  
pp. 196-209
Author(s):  
Mingyue Lyu ◽  
Yunfei Zheng ◽  
Lingfei Jia ◽  
Yan Zheng ◽  
Yanping Liu ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Genome Wide ◽  
Marrow Mesenchymal Stem Cells ◽  
Titanium Surfaces

scholarly journals DNA methylation microarray uncovers a permissive methylome for cardiomyocyte differentiation in human mesenchymal stem cells

Genomics ◽  
2020 ◽  
Vol 112 (2) ◽  
pp. 1384-1395 ◽  
Author(s):  
Kavitha Govarthanan ◽  
Piyush Kumar Gupta ◽  
Deepa Ramasamy ◽  
Pavitra Kumar ◽  
Shobana Mahadevan ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Cardiomyocyte Differentiation

Matrix elasticity, replicative senescence and DNA methylation patterns of mesenchymal stem cells

Biomaterials ◽  
2014 ◽  
Vol 35 (24) ◽  
pp. 6351-6358 ◽  
Author(s):  
Anne Schellenberg ◽  
Sylvia Joussen ◽  
Kristin Moser ◽  
Nico Hampe ◽  
Nils Hersch ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Replicative Senescence ◽  
Matrix Elasticity ◽  
Methylation Patterns

scholarly journals Integrated analysis of DNA methylome and transcriptome reveals the differences in biological characteristics of porcine mesenchymal stem cells from bone marrow and umbilical cord

2019 ◽  
Author(s):  
Yalan Yang ◽  
Zhiguo Liu ◽  
Weimin Zhao ◽  
Lei Huang ◽  
Tianwen Wu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Expression Profiles ◽  
Biological Characteristics ◽  
Integrated Analysis ◽  
Differentiation Potential

Abstract Background Bone marrow (BM) and umbilical cord (UC) are the main sources of mesenchymal stem cells (MSCs). These two MSCs display significant differences in many biological characteristics, yet the underlying molecular mechanisms need to be explored. Results In this study, to better understanding the biological features of MSCs, we isolated BMMSCs and UCMSCs from inbred Wuzhishan miniature pigs and generated the first global DNA methylation and gene expression profiles of porcine MSCs. The results showed that the osteogenic and adipogenic differentiation ability of porcine BMMSCs is stronger than that of UCMSCs. Stem cell surface marker CD90 were positively detected in both BMMSCs and UCMSCs. 587 genes were differentially methylated (280 hypermethylated and 307 hypomethylated) at the promoter regions between BMMSCs and UCMSCs. Meanwhile, 1,979 differentially expressed genes (1,407 up-regulated and 572 down-regulated) were identified between BMMSCs and UCMSCs. Integrative analysis reveals that 120 genes displayed differences in both gene expression and promoter methylation. Gene Ontology enrichment analysis revealed that these differential genes were associated with cell differentiation, cell migration, and immunogenicity properties. Remarkably, skeletal system development related genes were significantly hypomethylated and up-regulated in UCMSCs, while cell cycle genes were significantly higher down-regulated and hypermethylated, implying UCMSCs have higher cell proliferative activity and lower osteogenic differentiation potential than BMMSCs. Conclusions Our results indicate that DNA methylation plays an important role in regulating the biological characteristics differences between BMMSCs and UCMSCs. The study might provide a molecular theory basis for the application of porcine MSCs in human.

scholarly journals Replicative senescence of mesenchymal stem cells causes DNA-methylation changes which correlate with repressive histone marks

Aging ◽  
2011 ◽  
Vol 3 (9) ◽  
pp. 873-888 ◽  
Author(s):  
Anne Schellenberg ◽  
Qiong Lin ◽  
Herdit Schüler ◽  
Carmen M. Koch ◽  
Sylvia Joussen ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Replicative Senescence ◽  
Histone Marks

scholarly journals Does DNA Methylation Plays a Critical Role in Osteoblastic Differentiation of Mesenchymal Stem Cells (MSCs)?

2013 ◽  
Vol 15 (8) ◽  
pp. 755-756 ◽  
Author(s):  
Najmaldin Saki ◽  
Majid Farshdousti Hagh ◽  
Esmaeil Mortaz ◽  
Abdolreza Ardeshiry Lajimi
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Critical Role ◽  
Osteoblastic Differentiation

scholarly journals Landscape of transcription and expression regulated by DNA methylation related to age of donor and cell passage in adipose-derived mesenchymal stem cells

Aging ◽  
2020 ◽  
Vol 12 (21) ◽  
pp. 21186-21201
Author(s):  
Guan-Ming Lu ◽  
Yong-Xian Rong ◽  
Zhi-Jie Liang ◽  
Dong-lin Hunag ◽  
Yan-Fei Ma ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Passage
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