scholarly journals Anti-proliferative effects of γ-tocotrienol are associated with suppression of c-Myc expression in mammary tumour cells

2015 ◽  
Vol 48 (4) ◽  
pp. 421-435 ◽  
Author(s):  
P. Parajuli ◽  
R. V. Tiwari ◽  
P. W. Sylvester
Keyword(s):  
Tumour Cells ◽  
Mammary Tumour

scholarly journals Effect of dual agonists on phosphoinositide pools in WRK-1 cells

1990 ◽  
Vol 269 (3) ◽  
pp. 633-637 ◽  
Author(s):  
M E Monaco ◽  
M Attinasi ◽  
K Koréh
Keyword(s):  
Time Course ◽  
Inositol Phosphates ◽  
Subsequent Treatment ◽  
Tumour Cells ◽  
The Other ◽  
Mammary Tumour ◽  
Lipid Pool ◽  
Phosphoinositide Cycle ◽  
Heterologous Desensitization ◽  
Hormone Sensitive

Both vasopressin and bradykinin activate the phosphoinositide cycle in WRK-1 rat mammary tumour cells. When the two agonists are added simultaneously, partial additivity is observed with respect to disappearance of prelabelled phosphoinositides and accumulation of inositol phosphates; no additivity is observed with respect to resynthesis of phosphatidylinositol as assessed by monitoring [32P]Pi incorporation. Lack of complete additivity can be explained, at least in part, by heterologous desensitization. In order to determine whether the two agonists were accessing a common or individual hormone-sensitive phosphoinositide pools, cells were incubated with [32P]Pi in the presence of either vasopressin or bradykinin and subsequently restimulated with the alternative agonist. The lipid pool labelled in the presence of either agonist was sensitive to subsequent treatment by the other ligand, suggesting a common phosphoinositide pool. However, when cells were incubated with [32P]Pi in the absence of agonists, the time course of labelling of the hormone-sensitive pool was different for bradykinin and vasopressin, with that for bradykinin becoming labelled within a much shorter time. Thus although there is a significant overlap between the phosphoinositide pools responding to vasopressin and bradykinin, there is a small fraction of the hormone-sensitive lipid which responds only to bradykinin.

scholarly journals Isolated Flavonoids against Mammary Tumour Cells LM2

2009 ◽  
Vol 64 (1-2) ◽  
pp. 32-36 ◽  
Author(s):  
Camila B. de A. Carli ◽  
Djamile C. de Matos ◽  
Flávia C. M. Lopes ◽  
Danielle C. G. Maia ◽  
Maristela B. Dias ◽  
...  
Keyword(s):  
Antitumour Activity ◽  
Tumour Necrosis Factor Alpha ◽  
Tumour Cells ◽  
Mammary Tumour ◽  
Sandwich Assay ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Factor Alpha ◽  
Almost All ◽  
Necrosis Factor

1The purpose of the present study was to investigate antitumour and anti-inflammatory activities of flavonoids isolated from Byrsonima crassa, Davilla elliptica and Mouriri pusa. The antitumour activity was measured by the MTT assay in murine mammary tumour cells (LM2) and the IC50 values of the fl avonoids tested ranged from (31.5 ± 2.97) to (203.1 ± 5.9) μg/ml. The fl avonoids (myricetin-3-O-α-L-rhamnopyranoside) and 3 (quercetin-3-Ogalactopyranoside) from D. elliptica were the most active ones against the tumour cells. The same samples were tested to determine the inhibition of the release of nitric oxide (NO) and of the tumour necrosis factor-alpha (TNF-α) in murine macrophages by the Griess and ELISA sandwich assay, respectively. Almost all the samples showed inhibitory activity to the release of NO but not of TNF-α. Of all substances tested, flavonoids 2 (quercetin) and 6 (myricetin) may show promising activity in the treatment of murine breast cancer by immunomodulatory and antiproliferative activities.

Murine Mammary Tumour Cells In Vitro. Ii. Recruitment of Quiescent Cells

1984 ◽  
Vol 17 (1) ◽  
pp. 79-89
Author(s):  
C. Anne Wallen ◽  
R. Higashikubo ◽  
L. A. Dethlefsen
Keyword(s):  
Tumour Cells ◽  
Mammary Tumour ◽  
Quiescent Cells

scholarly journals Phorbol ester inhibition of the hormone-stimulated phosphoinositide cycle in WRK-1 cells

1986 ◽  
Vol 236 (1) ◽  
pp. 171-175 ◽  
Author(s):  
M E Monaco ◽  
R A Mufson
Keyword(s):  
Binding Affinity ◽  
Phorbol Ester ◽  
Inositol Phosphates ◽  
Inositol Phosphate ◽  
Tumour Cells ◽  
Mammary Tumour ◽  
Phosphate Accumulation ◽  
Phosphoinositide Cycle ◽  
Inositol Phosphate Accumulation

WRK-1 rat mammary tumour cells respond to vasopressin with increased accumulation of inositol phosphates as well as increased precursor incorporation into phosphatidylinositol. The phorbol ester, phorbol 13-myristate 12-acetate (PMA) inhibits by 80% both inositol phosphate accumulation and increased precursor incorporation. This inhibition is much less evident at early times (2 min) than at later times (25 min). The vasopressin-induced rise in cytosolic free Ca2+ is inhibited in a similar manner. Oleoylacetylglycerol is inactive with respect to inhibition of vasopressin-induced increases in incorporation of 32P into phosphoinositides. PMA has no effect on vasopressin binding at saturating concentrations of the hormone and does not affect the binding affinity.

Murine Mammary Tumour Cells In Vitro. I. the Development of A Quiescent State

1984 ◽  
Vol 17 (1) ◽  
pp. 65-77 ◽  
Author(s):  
C. Anne Wallen ◽  
R. Higashikubo ◽  
L. A. Dethlefsen
Keyword(s):  
Tumour Cells ◽  
Mammary Tumour ◽  
Quiescent State

Direct effect of a gonadotropin-releasing hormone agonist on the growth of canine mammary tumour cells

2002 ◽  
Vol 85 (3) ◽  
pp. 470-481 ◽  
Author(s):  
Ugo Pagnini ◽  
Salvatore Florio ◽  
Luca Crispino ◽  
Giuseppe Pagnini ◽  
Donato Colangelo ◽  
...  
Keyword(s):  
Direct Effect ◽  
Gonadotropin Releasing Hormone ◽  
Tumour Cells ◽  
Mammary Tumour ◽  
Releasing Hormone ◽  
Canine Mammary Tumour ◽  
Gonadotropin Releasing Hormone Agonist
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