scholarly journals Nimbolide inhibits invasion and migration, and down-regulates uPAR chemokine gene expression, in two breast cancer cell lines

2014 ◽  
Vol 47 (6) ◽  
pp. 540-552 ◽  
Author(s):  
P. Elumalai ◽  
A. Brindha Mercy ◽  
R. Arunkamar ◽  
G. Sharmila ◽  
F. A. Bhat ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Lines ◽  
Chemokine Gene ◽  
Invasion And Migration ◽  
And Migration ◽  
Chemokine Gene Expression

scholarly journals Gene expression profiling of breast cancer cell lines treated with proton and electron radiations

2018 ◽  
pp. 20170934 ◽  
Author(s):  
Valentina Bravatà ◽  
Luigi Minafra ◽  
Francesco Paolo Cammarata ◽  
Pietro Pisciotta ◽  
Debora Lamia ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Gene Expression Profiling ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Expression Profiling ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines

scholarly journals Human Arylamine N-Acetyltransferase 1 (NAT1) Knockout in MDA-MB-231 Breast Cancer Cell Lines Leads to Transcription of NAT2

2022 ◽  
Vol 12 ◽  
Author(s):  
Samantha M. Carlisle ◽  
Patrick J. Trainor ◽  
Mark A. Doll ◽  
David W. Hein
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Differential Expression ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Global Gene Expression ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Metabolizing Enzyme

Many cancers, including breast cancer, have shown differential expression of human arylamine N-acetyltransferase 1 (NAT1). The exact effect this differential expression has on disease risk and progression remains unclear. While NAT1 is classically defined as a xenobiotic metabolizing enzyme, other functions and roles in endogenous metabolism have recently been described providing additional impetus for investigating the effects of varying levels of NAT1 on global gene expression. Our objective is to further evaluate the role of NAT1 in breast cancer by determining the effect of NAT1 overexpression, knockdown, and knockout on global gene expression in MDA-MB-231 cell lines. RNA-seq was utilized to interrogate differential gene expression (genes correlated with NAT1 activity) across three biological replicates of previously constructed and characterized MDA-MB-231 breast cancer cell lines expressing parental (Scrambled), increased (Up), decreased (Down, CRISPR 2–12), or knockout (CRISPR 2–19, CRISPR 5–50) levels of NAT1. 3,889 genes were significantly associated with the NAT1 N-acetylation activity of the cell lines (adjusted p ≤ 0.05); of those 3,889 genes, 1,756 were positively associated with NAT1 N-acetylation activity and 2,133 were negatively associated with NAT1 N-acetylation activity. An enrichment of genes involved in cell adhesion was observed. Additionally, human arylamine N-acetyltransferase 2 (NAT2) transcripts were observed in the complete NAT1 knockout cell lines (CRISPR 2–19 and CRISPR 5–50). This study provides further evidence that NAT1 functions as more than just a drug metabolizing enzyme given the observation that differences in NAT1 activity have significant impacts on global gene expression. Additionally, our data suggests the knockout of NAT1 results in transcription of its isozyme NAT2.

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