scholarly journals Combined γ-tocotrienol and Met inhibitor treatment suppresses mammary cancer cell proliferation, epithelial-to-mesenchymal transition and migration

2013 ◽  
pp. n/a-n/a ◽  
Author(s):  
N. M. Ayoub ◽  
M. R. Akl ◽  
P. W. Sylvester
Keyword(s):  
Cell Proliferation ◽  
Cancer Cell ◽  
Mammary Cancer ◽  
Epithelial To Mesenchymal Transition ◽  
Cancer Cell Proliferation ◽  
Mesenchymal Transition ◽  
Mammary Cancer Cell ◽  
Met Inhibitor ◽  
And Migration ◽  
Inhibitor Treatment

Exercise-induced muscle-derived cytokines inhibit mammary cancer cell growth

2011 ◽  
Vol 301 (3) ◽  
pp. E504-E510 ◽  
Author(s):  
Pernille Hojman ◽  
Christine Dethlefsen ◽  
Claus Brandt ◽  
Jakob Hansen ◽  
Line Pedersen ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cancer Cell ◽  
Mammary Cancer ◽  
Exercise Bout ◽  
Cancer Cell Proliferation ◽  
Caspase Activity ◽  
Humoral Factors ◽  
Mammary Cancer Cell ◽  
Exercise Induced ◽  
Mcf 7

Regular physical activity protects against the development of breast and colon cancer, since it reduces the risk of developing these by 25–30%. During exercise, humoral factors are released from the working muscles for endocrinal signaling to other organs. We hypothesized that these myokines mediate some of the inhibitory effects of exercise on mammary cancer cell proliferation. Serum and muscles were collected from mice after an exercise bout. Incubation with exercise-conditioned serum inhibited MCF-7 cell proliferation by 52% and increased caspase activity by 54%. A similar increase in caspase activity was found after incubation of MCF-7 cells with conditioned media from electrically stimulated myotubes. PCR array analysis (CAPM-0838E; SABiosciences) revealed that seven genes were upregulated in the muscles after exercise, and of these oncostatin M (OSM) proved to inhibit MCF-7 proliferation by 42%, increase caspase activity by 46%, and induce apoptosis. Blocking OSM signaling with anti-OSM antibodies reduced the induction of caspase activity by 51%. To verify that OSM was a myokine, we showed that it was significantly upregulated in serum and in three muscles, tibialis cranialis, gastronemius, and soleus, after an exercise bout. In contrast, OSM expression remained unchanged in subcutaneous and visceral adipose tissue, liver, and spleen (mononuclear cells). We conclude that postexercise serum inhibits mammary cancer cell proliferation and induces apoptosis of these cells. We suggest that one or more myokines secreted from working muscles may be mediating this effect and that OSM is a possible candidate. These findings emphasize that role of physical activity in cancer treatment, showing a direct link between exercise-induced humoral factors and decreased tumor cell growth.

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