scholarly journals Effects of serum reduction and VEGF supplementation on angiogenic potential of human adipose stromal cells in vitro

2013 ◽  
Vol 46 (3) ◽  
pp. 300-311 ◽  
Author(s):  
K. H. Chua ◽  
F. Raduan ◽  
W. K. Z. Wan Safwani ◽  
N. F. M. Manzor ◽  
B. Pingguan‐Murphy ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Adipose Stromal Cells ◽  
Angiogenic Potential ◽  
Serum Reduction

Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo

2005 ◽  
Vol 328 (1) ◽  
pp. 258-264 ◽  
Author(s):  
Min Jeong Seo ◽  
Su Young Suh ◽  
Yong Chan Bae ◽  
Jin Sup Jung
Keyword(s):  
Stromal Cells ◽  
Adipose Stromal Cells

Angiogenic Potential of Multipotent Stromal Cells from the Umbilical Cord: an In Vitro Study

2016 ◽  
Vol 161 (1) ◽  
pp. 141-149
Author(s):  
I. V. Arutyunyan ◽  
E. Yu. Kananykhina ◽  
T. Kh. Fatkhudinov ◽  
A. V. El’chaninov ◽  
A. V. Makarov ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Stromal Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Multipotent Stromal Cells ◽  
Angiogenic Potential

Interactions between human adipose stromal cells and mouse neural stem cells in vitro

2003 ◽  
Vol 145 (1) ◽  
pp. 141-149 ◽  
Author(s):  
Soo Kyung Kang ◽  
Eun Sook Jun ◽  
Yong Chan Bae ◽  
Jin Sup Jung
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Stromal Cells ◽  
Adipose Stromal Cells

scholarly journals Intrinsic Angiogenic Potential and Migration Capacity of Human Mesenchymal Stromal Cells Derived from Menstrual Blood and Bone Marrow

2020 ◽  
Vol 21 (24) ◽  
pp. 9563
Author(s):  
Rosana de Almeida Santos ◽  
Karina Dutra Asensi ◽  
Julia Helena Oliveira de Barros ◽  
Rafael Campos Silva de Menezes ◽  
Ingrid Rosenburg Cordeiro ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
3D Culture ◽  
Menstrual Blood ◽  
Paracrine Effects ◽  
Angiogenic Potential ◽  
And Migration

Several therapies are being developed to increase blood circulation in ischemic tissues. Despite bone marrow-derived mesenchymal stromal cells (bmMSC) are still the most studied, an interesting and less invasive MSC source is the menstrual blood, which has shown great angiogenic capabilities. Therefore, the aim of this study was to evaluate the angiogenic properties of menstrual blood-derived mesenchymal stromal cells (mbMSC) in vitro and in vivo and compared to bmMSC. MSC’s intrinsic angiogenic capacity was assessed by sprouting and migration assays. mbMSC presented higher invasion and longer sprouts in 3D culture. Additionally, both MSC-spheroids showed cells expressing CD31. mbMSC and bmMSC were able to migrate after scratch wound in vitro, nonetheless, only mbMSC demonstrated ability to engraft in the chick embryo, migrating to perivascular, perineural, and chondrogenic regions. In order to study the paracrine effects, mbMSC and bmMSC conditioned mediums were capable of stimulating HUVEC’s tube-like formation and migration. Both cells expressed VEGF-A and FGF2. Meanwhile, PDGF-B was expressed exclusively in mbMSC. Our results indicated that mbMSC and bmMSC presented a promising angiogenic potential. However, mbMSC seems to have additional advantages since it can be obtained by non-invasive procedure and expresses PDGF-B, an important molecule for vascular formation and remodeling.

scholarly journals Angiogenic Potential of Human Neonatal Foreskin Stromal Cells in the Chick Embryo Chorioallantoic Membrane Model

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Radhakrishnan Vishnubalaji ◽  
Muhammad Atteya ◽  
May Al-Nbaheen ◽  
Richard O. C. Oreffo ◽  
Abdullah Aldahmash ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Ex Vivo ◽  
Smooth Muscle Actin ◽  
Chorioallantoic Membrane ◽  
Cell Types ◽  
Factor Xiiia ◽  
Potential Contribution ◽  
Angiogenic Potential ◽  
Undifferentiated State

Several studies have demonstrated the multipotentiality of human neonatal foreskin stromal cells (hNSSCs) as being able to differentiate into adipocytes and osteoblasts and potentially other cell types. Recently, we demonstrated that hNSSCs play a role duringin vitroangiogenesis and appear to possess a capacity to differentiate into endothelial-like cells; however, their angiogenic potential within anex vivoenvironment remains unclear. Current study shows hNSSCs to display significant migration potential in the undifferentiated state and high responsiveness in thein vitrowound healing scratch assay. When hNSSCs were seeded onto the top of the CAM, human von Willebrand factor (hVWF), CD31, smooth muscle actin (SMA), and factor XIIIa positive cells were observed in the chick endothelium. CAMs transplanted with endothelial-differentiated hNSSCs displayed a higher number of blood vessels containing hNSSCs compared to CAMs transplanted with undifferentiated hNSSCs. Interestingly, undifferentiated hNSSCs showed a propensity to differentiate towards ectoderm with indication of epidermal formation with cells positive for CD1a, CK5/6, CK19, FXIIIa, and S-100 cells, which warrant further investigation. Our findings imply a potential angiogenic role for hNSSCsex vivoin the differentiated and undifferentiated state, with potential contribution to blood vessel formation and potential application in tissue regeneration and vascularization.

scholarly journals Human Adipose Stromal Cells (ASC) for the Regeneration of Injured Cartilage Display Genetic Stability after In Vitro Culture Expansion

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e77895 ◽  
Author(s):  
Simona Neri ◽  
Philippe Bourin ◽  
Julie-Anne Peyrafitte ◽  
Luca Cattini ◽  
Andrea Facchini ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Stromal Cells ◽  
Genetic Stability ◽  
Adipose Stromal Cells

scholarly journals Pentraxin 3 Inhibits the Angiogenic Potential of Multiple Myeloma Cells

Cancers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 2255
Author(s):  
Roberto Ronca ◽  
Sara Taranto ◽  
Michela Corsini ◽  
Chiara Tobia ◽  
Cosetta Ravelli ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Stromal Cells ◽  
Plasma Cells ◽  
In Vitro Models ◽  
Tumor Burden ◽  
Pentraxin 3 ◽  
In Vivo Models ◽  
Angiogenic Potential

During multiple myeloma (MM) progression the activation of the angiogenic process represents a key step for the formation of the vascular niche, where different stromal components and neoplastic cells collaborate and foster tumor growth. Among the different pro-angiogenic players, Fibroblast Growth Factor 2 (FGF2) plays a pivotal role in BM vascularization occurring during MM progression. Long Pentraxin 3 (PTX3), a natural FGF antagonist, is able to reduce the activation of stromal components promoted by FGF2 in various in vitro models. An increased FGF/PTX3 ratio has also been found to occur during MM evolution, suggesting that restoring the “physiological” FGF/PTX3 ratio in plasma cells and BM stromal cells (BMSCs) might impact MM. In this work, taking advantage of PTX3-inducible human MM models, we show that PTX3 produced by tumor cells is able to restore a balanced FGF/PTX3 ratio sufficient to prevent the activation of the FGF/FGFR system in endothelial cells and to reduce the angiogenic capacity of MM cells in different in vivo models. As a result of this anti-angiogenic activity, PTX3 overexpression causes a significant reduction of the tumor burden in both subcutaneously grafted and systemic MM models. These data pave the way for the exploitation of PTX3-derived anti-angiogenic approaches in MM.

scholarly journals Decidua Parietalis Mesenchymal Stem/Stromal Cells and Their Secretome Diminish the Oncogenic Properties of MDA231 Cells In Vitro

Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3493
Author(s):  
Yasser Basmaeil ◽  
Eman Bahattab ◽  
Abdullah Al Subayyil ◽  
Haya Kulayb ◽  
Maha Alrodayyan ◽  
...  
Keyword(s):  
Cell Line ◽  
Stromal Cells ◽  
Cancer Cell Line ◽  
Migration And Invasion ◽  
Biological Functions ◽  
Human Breast ◽  
Angiogenic Potential ◽  
Cell Based Therapy ◽  
Apoptotic Genes

Mesenchymal stem cells (MSCs) have been shown to suppress tumor growth, inhibit angiogenesis, regulate cellular signaling, and induce apoptosis in cancer cells. We have earlier reported that placenta-derived decidua parietalis mesenchymal stem/stromal cells (DPMSCs) not only retained their functional characteristics in the cancer microenvironment but also exhibited increased expression of anti-apoptotic genes, demonstrating their anti-tumor properties in the tumor setting. In this study, we have further evaluated the effects of DPMSCs on the functional outcome of human breast cancer cell line MDA231. MDA231 cells were exposed to DPMSCs, and their biological functions, including adhesion, proliferation, migration, and invasion, were evaluated. In addition, genomic and proteomic modifications of the MDA231 cell line, in response to the DPMSCs, were also evaluated. MDA231 cells exhibited a significant reduction in proliferation, migration, and invasion potential after their treatment with DPMSCs. Furthermore, DPMSC treatment diminished the angiogenic potential of MDA231 cells. DPMSC treatment modulated the expression of various pro-apoptotic as well as oncogenes in MDA231 cells. The properties of DPMSCs to inhibit the invasive characteristics of MDA231 cells demonstrate that they may be a useful candidate in a stem-cell-based therapy against cancer.

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