Methylene blue submucosal infiltration may facilitate transanal submucosal dissection

2017 ◽  
Vol 19 (11) ◽  
pp. 1032-1033 ◽  
Author(s):  
T. J. Cuda ◽  
A. D. Riddell ◽  
D. A. Westwood ◽  
A. E. R. Hamilton ◽  
A. R. L. Stevenson
2017 ◽  
Vol 19 (11) ◽  
pp. 1033-1033
Author(s):  
T. J. Cuda ◽  
D. A. Westwood ◽  
A. E. R. Hamilton ◽  
A. R. L. Stevenson

Author(s):  
B. J. Panessa ◽  
J. F. Gennaro

Tissue from the hood and sarcophagus regions were fixed in 6% glutaraldehyde in 1 M.cacodylate buffer and washed in buffer. Tissue for SEM was partially dried, attached to aluminium targets with silver conducting paint, carbon-gold coated(100-500Å), and examined in a Kent Cambridge Stereoscan S4. Tissue for the light microscope was post fixed in 1% aqueous OsO4, dehydrated in acetone (4°C), embedded in Epon 812 and sectioned at ½u on a Sorvall MT 2 ultramicrotome. Cross and longitudinal sections were cut and stained with PAS, 0.5% toluidine blue and 1% azure II-methylene blue. Measurements were made from both SEM and Light micrographs.The tissue had two structurally distinct surfaces, an outer surface with small (225-500 µ) pubescent hairs (12/mm2), numerous stoma (77/mm2), and nectar glands(8/mm2); and an inner surface with large (784-1000 µ)stiff hairs(4/mm2), fewer stoma (46/mm2) and larger, more complex glands(16/mm2), presumably of a digestive nature.


2012 ◽  
Vol 60 (S 01) ◽  
Author(s):  
H Weiler ◽  
O Moeller ◽  
M Wohlhoefer ◽  
LO Conzelmann ◽  
J Albers ◽  
...  

Endoscopy ◽  
2013 ◽  
Vol 45 (11) ◽  
Author(s):  
F Baldaque-Silva ◽  
M Marques ◽  
F Vilas Boas ◽  
E Duarte ◽  
J Lopes ◽  
...  

2014 ◽  
Vol 62 (S 01) ◽  
Author(s):  
I. Kanzler ◽  
F. Guo ◽  
N. Bogert ◽  
A. Moritz ◽  
A. Beiras-Fernandez

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