Rapid allergen-induced interleukin-17 and interferon-γ secretion by skin-resident memory CD8+T cells

2016 ◽  
Vol 76 (4) ◽  
pp. 218-227 ◽  
Author(s):  
Jonas D. Schmidt ◽  
Malin G. Ahlström ◽  
Jeanne D. Johansen ◽  
Beatrice Dyring-Andersen ◽  
Christina Agerbeck ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Interferon Γ ◽  
Interleukin 17 ◽  
Memory Cd8 T Cells

scholarly journals Differential Antigen Specificity of Hepatitis C Virus–Specific Interleukin 17– and Interferon γ–Producing CD8+ T Cells During Chronic Infection

2012 ◽  
Vol 205 (7) ◽  
pp. 1142-1146 ◽  
Author(s):  
Stefanie Grafmueller ◽  
Eva Billerbeck ◽  
Hubert E. Blum ◽  
Christoph Neumann-Haefelin ◽  
Robert Thimme
Keyword(s):  
T Cells ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cd8 T Cells ◽  
Chronic Infection ◽  
Interferon Γ ◽  
Interleukin 17 ◽  
Antigen Specificity

scholarly journals Memory CD8 + T cells in HIV infection

2000 ◽  
Vol 355 (1395) ◽  
pp. 363-367 ◽  
Author(s):  
Andrew J. McMichael ◽  
Graham Ogg ◽  
Jamie Wilson ◽  
Margaret Callan ◽  
Sophie Hambleton ◽  
...  
Keyword(s):  
T Cells ◽  
Hiv Infection ◽  
Cd8 T Cells ◽  
T Helper Cells ◽  
Hla Class I ◽  
Interferon Γ ◽  
Virus Infections ◽  
T Helper ◽  
Memory Cd8 T Cells ◽  
Turn Over

Cytotoxic T lymphocytes (CTLs) play a central role in the control of persistent HIV infection in humans. The kinetics and general features of the CTL response are similar to those found during other persisting virus infections in humans. During chronic infection there are commonly between 0.1 and 1.0% of all CD8 + T cells in the blood that are specific for immunodominant virus epitopes, as measured by HLA class I peptide tetramers. These figures are greatly in excess of the numbers found by limiting dilution assays; the discrepancy may arise because in the latter assay, CTLs have to divide many times to be detected and many of the HIV–specific CD8 + T cells circulating in infected persons may be incapable of further division. Many tetramer–positive T cells make interferon–γ, β–chemokines and perforin, so are probably functional. It is not known how fast these T cells turn over, but in the absence of antigen they decay in number. Impairment of CTL replacement, because CD4 + T helper cells are depleted by HIV infection, may play a major role in the development of AIDS.

scholarly journals Memory CD8+ T cells mediate antibacterial immunity via CCL3 activation of TNF/ROI+ phagocytes

2007 ◽  
Vol 204 (9) ◽  
pp. 2075-2087 ◽  
Author(s):  
Emilie Narni-Mancinelli ◽  
Laura Campisi ◽  
Delphine Bassand ◽  
Julie Cazareth ◽  
Pierre Gounon ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Secondary Infection ◽  
Interferon Γ ◽  
Phagocytic Cells ◽  
Oxygen Intermediates ◽  
Therapeutic Vaccines ◽  
Memory Cd8 T Cells ◽  
Tnf Α

Cytolysis, interferon γ and tumor necrosis factor (TNF) α secretion are major effector mechanisms of memory CD8+ T cells that are believed to be required for immunological protection in vivo. By using mutants of the intracellular bacterium Listeria monocytogenes, we found that none of these effector activities is sufficient to protect against secondary infection with wild-type (WT) bacteria. We demonstrated that CCL3 derived from reactivated memory CD8+ T cells is required for efficient killing of WT bacteria. CCL3 induces a rapid TNF-α secretion by innate inflammatory mononuclear phagocytic cells (MPCs), which further promotes the production of radical oxygen intermediates (ROIs) by both MPCs and neutrophils. ROI generation is the final bactericidal mechanism involved in L. monocytogenes clearance. These results therefore uncover two levels of regulation of the antibacterial secondary protective response: (a) an antigen-dependent phase in which memory CD8+ T cells are reactivated and control the activation of the innate immune system, and (b) an antigen-independent phase in which the MPCs coordinate innate immunity and promote the bactericidal effector activities. In this context, CCL3-secreting memory CD8+ T cells are able to mediate “bystander” killing of an unrelated pathogen upon antigen-specific reactivation, a mechanism that may be important for the design of therapeutic vaccines.

scholarly journals IL-15 induces antigen-independent expansion and differentiation of human naive CD8+ T cells in vitro

Blood ◽  
2003 ◽  
Vol 102 (7) ◽  
pp. 2541-2546 ◽  
Author(s):  
Nuno L. Alves ◽  
Berend Hooibrink ◽  
Fernando A. Arosa ◽  
René A. W. van Lier
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cd8 T Cells ◽  
Cd8 T Cell ◽  
Interferon Γ ◽  
T Cell Subsets ◽  
Memory Cd8 T Cells ◽  
Effector Cells ◽  
Independent Expansion

Abstract Recent studies in mice have shown that although interleukin 15 (IL-15) plays an important role in regulating homeostasis of memory CD8+ T cells, it has no apparent function in controlling homeostatic proliferation of naive T cells. We here assessed the influence of IL-15 on antigen-independent expansion and differentiation of human CD8+ T cells. Both naive and primed human T cells divided in response to IL-15. In this process, naive CD8+ T cells successively down-regulated CD45RA and CD28 but maintained CD27 expression. Concomitant with these phenotypic changes, naive cells acquired the ability to produce interferon γ (IFN-γ) and tumor necrosis factor α (TNF-α), expressed perforin and granzyme B, and acquired cytotoxic properties. Primed CD8+ T cells, from both noncytotoxic (CD45RA-CD27+) and cytotoxic (CD45RA+CD27-) subsets, responded to IL-15 and yielded ample numbers of cytokine-secreting and cytotoxic effector cells. In summary, all human CD8+ T-cell subsets had the ability to respond to IL-15, which suggests a generic influence of this cytokine on CD8+ T-cell homeostasis in man. (Blood. 2003;102:2541-2546)

scholarly journals Type 17 CD8+ T cells display enhanced antitumor immunity

Blood ◽  
2009 ◽  
Vol 114 (3) ◽  
pp. 596-599 ◽  
Author(s):  
Christian S. Hinrichs ◽  
Andrew Kaiser ◽  
Chrystal M. Paulos ◽  
Lydie Cassard ◽  
Luis Sanchez-Perez ◽  
...  
Keyword(s):  
T Cells ◽  
Cancer Therapy ◽  
Cd8 T Cells ◽  
Antitumor Immunity ◽  
Killer Cell ◽  
Cd8 T Cell ◽  
Interferon Γ ◽  
Interleukin 17 ◽  
Effector Cells

Abstract Interleukin-17 (IL-17)–secreting CD8+ T cells have been described, but they have not been thoroughly studied and they do not have a known role in cancer immunotherapy. We skewed CD8+ T cells to secrete IL-17 through priming in Th17-polarizing conditions. IL-17–producing CD8+ T cells demonstrated reduced expression of Eomes and diminished cytolytic differentiation in vitro. However, after adoptive transfer, these cells converted to interferon-γ–producing effector cells and mediated regression of large, established tumors. This improved antitumor immunity was associated with increased expression of IL-7R-alpha, decreased expression of killer cell lectin-like receptor G1, and enhanced persistence of the transferred cells. This report is the first description of a cancer therapy with IL-17–secreting CD8+ T cells. These findings have implications for the improvement of CD8+ T cell–based adoptive immunotherapy.

scholarly journals CD4+ and CD8+ T cells producing Th1 and Th17 cytokines are involved in the pathogenesis of autoimmune orchitis

Reproduction ◽  
2011 ◽  
Vol 141 (2) ◽  
pp. 249-258 ◽  
Author(s):  
Patricia Jacobo ◽  
Cecilia Valeria Pérez ◽  
María Susana Theas ◽  
Vanesa Anabella Guazzone ◽  
Livia Lustig
Keyword(s):  
T Cells ◽  
Western Blot ◽  
Cd8 T Cells ◽  
Chronic Phase ◽  
Interferon Γ ◽  
Seminiferous Tubules ◽  
Interleukin 17 ◽  
Effector Cells ◽  
Th17 Cytokines ◽  
Autoimmune Orchitis

Experimental autoimmune orchitis (EAO) is a useful model to study chronic testicular inflammation and infertility. EAO is characterized by severe damage of seminiferous tubules with germ cells that undergo apoptosis and sloughing. We previously reported an increase in CD4+ and CD8+ effector T cells in the testes of rats with EAO. Since cytokine patterns determine T cell effector functions, in the present work we analyzed the cytokines expressed by these cells during disease development. By flow cytometry, we detected an increase in the number of tumor necrosis factor-α (TNF) and interferon -γ (IFNG)-producing CD4+ T cells in the testis at EAO onset. As the severity of the disease progressed, these cells declined while CD8+ T cells producing TNF and IFNG increased, with the predominance of IFNG expression. As a novel finding, we identified by immunofluorescence CD4+ interleukin 17 (IL17)+ and CD8+ IL17+ cells in the testes of EAO rats, with CD4+ and CD8+ T cells predominating at the onset and in the chronic phase of EAO respectively. Moreover, IL17 (western blot) and IL23 content (ELISA) increased in EAO, with maximum levels in the chronic phase. These results suggest the involvement of CD4+ T helper (Th) 1 and Th17 subsets as co-effector cells governing EAO onset, as well as the central contribution of CD8+ T cells producing Th1 and Th17 cytokines in the maintenance of chronic inflammation. The expression of T-bet and RORγt (western blot) in the testis over the course of disease also supports the presence of Th1 and Th17 cells in the testes of EAO rats.

Antigen cross-presentation in the liver generates protective memory CD8+ T cells in the absence of inflammation

2012 ◽  
Vol 50 (01) ◽  
Author(s):  
JP Böttcher ◽  
D Stabenow ◽  
S Debey-Pascher ◽  
A Staratschek-Jox ◽  
J Grell ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Memory Cd8 T Cells ◽  
Cross Presentation
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