scholarly journals Mitochondrial respiratory chain complex enzyme activities of limbic structures and psychiatric diagnosis in temporal lobe epilepsy patients: Preliminary results

2017 ◽  
Vol 23 (8) ◽  
pp. 700-702 ◽  
Author(s):  
Camila Moreira Osório ◽  
Kátia Lin ◽  
Ricardo Guarnieri ◽  
Maria Emília Rodrigues de Oliveira Thais ◽  
Helena Dresch Vascouto ◽  
...  
Keyword(s):  
Temporal Lobe Epilepsy ◽  
Respiratory Chain ◽  
Temporal Lobe ◽  
Enzyme Activities ◽  
Psychiatric Diagnosis ◽  
Chain Complex ◽  
Mitochondrial Respiratory Chain ◽  
Respiratory Chain Complex ◽  
Limbic Structures ◽  
Mitochondrial Respiratory Chain Complex

scholarly journals Automated Spectrophotometric Analysis of Mitochondrial Respiratory Chain Complex Enzyme Activities in Cultured Skin Fibroblasts

2005 ◽  
Vol 51 (11) ◽  
pp. 2110-2116 ◽  
Author(s):  
Karen A Kramer ◽  
Devin Oglesbee ◽  
Stacy J Hartman ◽  
Joe Huey ◽  
Bambi Anderson ◽  
...  
Keyword(s):  
Respiratory Chain ◽  
Enzyme Activities ◽  
Chain Complex ◽  
Mitochondrial Respiratory Chain ◽  
Skin Fibroblasts ◽  
Diagnostic Process ◽  
Respiratory Chain Complex ◽  
Mitochondrial Respiratory Chain Complex ◽  
Automated Method ◽  
Mitochondrial Respiratory

Abstract Background: Mitochondrial respiratory chain complex (RCC) disorders may occur as commonly as 1 in 8500 individuals. Because of the great variability of phenotypic presentations, measurement of individual RCC enzyme activities is a crucial diagnostic process. Current assay methods are time-consuming and labor-intensive and thus constitute a major impediment to clinical practice. A method with a faster turnaround time would therefore be beneficial. Method: We developed an automated spectrophotometric method for measuring the respiratory chain enzyme activities of complex I, complex II + III, and complex IV with the Hitachi 912, an automated spectrophotometer. Mitochondrial citrate synthase was also determined for normalization of the RCC activities. Results: A blinded method comparison with samples from an external testing center yielded a 91% concordance of interpretations. Mean intraassay imprecision (as CV; n = 20) in a single batch analysis of each RCC was 5.9%. Interassay imprecision, evaluated on 2 samples harvested and analyzed 3 times each, gave mean CVs of 10%–18%. Conclusions: With this automated method, a panel of RCC enzyme activities can be determined in <2 h. In addition, an immunoblot assay using monoclonal antibodies against specific subunits of RCC enzyme complexes can be informative in cases of borderline enzyme activity. Our results suggest that in vitro diagnosis of RCC enzyme deficiencies in skin fibroblasts is an effective alternative to invasive muscle biopsy.

Novel Mycotoxin fromAcremonium exuviarumIs a Powerful Inhibitor of the Mitochondrial Respiratory Chain Complex III

2009 ◽  
Vol 22 (3) ◽  
pp. 565-573 ◽  
Author(s):  
Alexey G. Kruglov ◽  
Maria A. Andersson ◽  
Raimo Mikkola ◽  
Merja Roivainen ◽  
Laszlo Kredics ◽  
...  
Keyword(s):  
Respiratory Chain ◽  
Chain Complex ◽  
Mitochondrial Respiratory Chain ◽  
Complex Iii ◽  
Respiratory Chain Complex ◽  
Mitochondrial Respiratory Chain Complex ◽  
Mitochondrial Respiratory
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