scholarly journals A crucial role for the C‐terminal domain of exported protein 1 during the mosquito and hepatic stages of the Plasmodium berghei life cycle

2019 ◽  
Vol 21 (10) ◽  
Author(s):  
Kamil Wolanin ◽  
Diana Fontinha ◽  
Margarida Sanches‐Vaz ◽  
Britta Nyboer ◽  
Kirsten Heiss ◽  
...  
Keyword(s):  
Life Cycle ◽  
Plasmodium Berghei ◽  
Crucial Role ◽  
Terminal Domain ◽  
Exported Protein

Mitochondrial apurinic/apyrimidinic endonuclease Apn1 is not critical for the completion of the Plasmodium berghei life cycle

DNA Repair ◽  
2021 ◽  
Vol 101 ◽  
pp. 103078 ◽  
Author(s):  
Pratik Narain Srivastava ◽  
Sunil Kumar Narwal ◽  
Satish Mishra
Keyword(s):  
Life Cycle ◽  
Plasmodium Berghei

Gene disruption reveals a dispensable role for Plasmepsin VII in the Plasmodium berghei life cycle

2014 ◽  
Vol 195 (1) ◽  
pp. 10-13 ◽  
Author(s):  
Babu S. Mastan ◽  
Anchala Kumari ◽  
Dinesh Gupta ◽  
Satish Mishra ◽  
Kota Arun Kumar
Keyword(s):  
Life Cycle ◽  
Plasmodium Berghei ◽  
Gene Disruption

scholarly journals The Crucial Role of Eosinophils in the Life Cycle, Radiographical Architecture, and Risk of Recurrence of Chronic Subdural Hematomas

2021 ◽  
Vol 2 (1) ◽  
pp. 76-83
Author(s):  
Benjamin Davidson ◽  
Karl Narvacan ◽  
David G. Munoz ◽  
Fabio Rotondo ◽  
Kalman Kovacs ◽  
...  
Keyword(s):  
Life Cycle ◽  
Crucial Role ◽  
Risk Of Recurrence ◽  
Subdural Hematomas

scholarly journals Structural Characterization of the Helicase nsp10 Encoded by Porcine Reproductive and Respiratory Syndrome Virus

2020 ◽  
Vol 94 (15) ◽  
Author(s):  
Yuejun Shi ◽  
Xiaohan Tong ◽  
Gang Ye ◽  
Ruixue Xiu ◽  
Lisha Li ◽  
...  
Keyword(s):  
Domain Structure ◽  
Crucial Role ◽  
Catalytic Domain ◽  
Full Length ◽  
Structural Basis ◽  
Economic Losses ◽  
Respiratory Syndrome Virus ◽  
Common Mechanism ◽  
Helicase Activity ◽  
Terminal Domain

ABSTRACT Currently, an effective therapeutic treatment for porcine reproductive and respiratory syndrome virus (PRRSV) remains elusive. PRRSV helicase nsp10 is an important component of the replication transcription complex that plays a crucial role in viral replication, making nsp10 an important target for drug development. Here, we report the first crystal structure of full-length nsp10 from the arterivirus PRRSV, which has multiple domains: an N-terminal zinc-binding domain (ZBD), a 1B domain, and helicase core domains 1A and 2A. Importantly, our structural analyses indicate that the conformation of the 1B domain from arterivirus nsp10 undergoes a dynamic transition. The polynucleotide substrate channel formed by domains 1A and 1B adopts an open state, which may create enough space to accommodate and bind double-stranded RNA (dsRNA) during unwinding. Moreover, we report a unique C-terminal domain structure that participates in stabilizing the overall helicase structure. Our biochemical experiments also showed that deletion of the 1B domain and C-terminal domain significantly reduced the helicase activity of nsp10, indicating that the four domains must cooperate to contribute to helicase function. In addition, our results indicate that nidoviruses contain a conserved helicase core domain and key amino acid sites affecting helicase function, which share a common mechanism of helicase translocation and unwinding activity. These findings will help to further our understanding of the mechanism of helicase function and provide new targets for the development of antiviral drugs. IMPORTANCE Porcine reproductive and respiratory syndrome virus (PRRSV) is a major respiratory disease agent in pigs that causes enormous economic losses to the global swine industry. PRRSV helicase nsp10 is a multifunctional protein with translocation and unwinding activities and plays a vital role in viral RNA synthesis. Here, we report the first structure of full-length nsp10 from the arterivirus PRRSV at 3.0-Å resolution. Our results show that the 1B domain of PRRSV nsp10 adopts a novel open state and has a unique C-terminal domain structure, which plays a crucial role in nsp10 helicase activity. Furthermore, mutagenesis and structural analysis revealed conservation of the helicase catalytic domain across the order Nidovirales (families Arteriviridae and Coronaviridae). Importantly, our results will provide a structural basis for further understanding the function of helicases in the order Nidovirales.

scholarly journals Advancements in mass spectrometry-based glycoproteomics and glycomics

2016 ◽  
Vol 3 (3) ◽  
pp. 345-364 ◽  
Author(s):  
Haojie Lu ◽  
Ying Zhang ◽  
Pengyuan Yang
Keyword(s):  
Mass Spectrometry ◽  
Life Cycle ◽  
Disease Progression ◽  
Crucial Role ◽  
Cell Development ◽  
Biological Processes ◽  
Development Life Cycle ◽  
Recent Developments ◽  
Made In ◽  
Research Studies

Abstract Protein N-glycosylation plays a crucial role in a considerable number of important biological processes. Research studies on glycoproteomes and glycomes have already characterized many glycoproteins and glycans associated with cell development, life cycle, and disease progression. Mass spectrometry (MS) is the most powerful tool for identifying biomolecules including glycoproteins and glycans, however, utilizing MS-based approaches to identify glycoproteomes and glycomes is challenging due to the technical difficulties associated with glycosylation analysis. In this review, we summarize the most recent developments in MS-based glycoproteomics and glycomics, including a discussion on the development of analytical methodologies and strategies used to explore the glycoproteome and glycome, as well as noteworthy biological discoveries made in glycoproteome and glycome research. This review places special emphasis on China, where scientists have made sizeable contributions to the literature, as advancements in glycoproteomics and glycomincs are occurring quite rapidly.

scholarly journals EBF recommendation on practical management of critical reagents for antidrug antibody ligand-binding assays

Bioanalysis ◽  
2019 ◽  
Vol 11 (19) ◽  
pp. 1787-1798 ◽  
Author(s):  
Susanne Pihl ◽  
Barry WA van der Strate ◽  
Michaela Golob ◽  
Janka Ryding ◽  
Laurent Vermet ◽  
...  
Keyword(s):  
Life Cycle ◽  
Ligand Binding ◽  
Crucial Role ◽  
Binding Assays ◽  
Antidrug Antibody ◽  
Regulatory Guidelines ◽  
Minimum Requirements ◽  
Antidrug Antibodies ◽  
Immunogenicity Assays

Immunogenicity (ISI) assays are required to measure antidrug antibodies that are generated against biotherapeutic modalities. As for any ligand-binding assays, critical reagents (CR) play a crucial role in immunogenicity assays, as the robustness and reliability of an assay are defined by the quality and long-term availability of these reagents. The current regulatory guidelines do not provide clear directions on how to implement and verify lot-to-lot changes of CR during an assay life cycle, or the acceptance criteria that should be used when implementing new lots of CR. These aspects were extensively discussed within the European Bioanalysis Forum community. In this paper, CR for immunogenicity assays are identified and the minimum requirements for introducing new lots of CR in immunogenicity assays are described.

scholarly journals Specific Business and Management Challenges in Globalised World: Opportunities and Recommendations

2015 ◽  
Vol 5 (2) ◽  
pp. 113
Author(s):  
MA. Arben Salihu
Keyword(s):  
Life Cycle ◽  
Human Resources ◽  
Crucial Role ◽  
Life Cycle Management ◽  
Careful Consideration ◽  
To Come ◽  
The Right ◽  
Business And Management ◽  
The Way

Globalisation is continually shaping the way we live, we travel and also the way and structure we work. It is generally acknowledged that there are a number of fundamental factors that play a crucial role in the business life cycle. Management is one of the main pillars of the organization because it provides direction, implementation, and coordination, so that organizations can attain their goals. An organisation’s life depends heavily on the quality of management. If there is any lack within the management element, it may severely limit an organisation’s existence. It is thus imperative to put all the elements in the right shape and place. Yet still this may be insufficient, due to ever growing competitiveness. Vision, strategy and innovation are fundamental in business enterprises but there are other issues related that have an effect to the management and need careful consideration. This study lists a number of specific challenges (namely leadership, innovation and human resources) that businesses and management is encountering and ought to be facing in several decades to come, and offers recommendation to the topics brought forward

Life Cycle and Patterns of Development of Plasmodium berghei in Normal and Experimental Hosts

1966 ◽  
Vol 131 (suppl_9) ◽  
pp. 900-914 ◽  
Author(s):  
M. Yoeli ◽  
R. S. Upmanis ◽  
J. Vanderberg ◽  
H. Most
Keyword(s):  
Life Cycle ◽  
Plasmodium Berghei

‘What a troupe family does’: Family as Transmission Narrative in the British Carnival Troupe Dancing Community

2019 ◽  
Vol 37 (1) ◽  
pp. 35-58
Author(s):  
Lucy Wright
Keyword(s):  
Life Cycle ◽  
Sense Of Community ◽  
Crucial Role ◽  
Rites Of Passage ◽  
High Status ◽  
Folk Dance ◽  
The North ◽  
The Family ◽  
Familial Relationship ◽  
Shared Context

Troupe dancing, also known as ‘entertaining’, is a competitive formational dance performed by girls and women in the North of England and Wales. Demanding significant discipline and commitment to achieve the high levels of synchronisation expected for a successful routine, participants demonstrate a strong sense of community, frequently describing their teammates in terms that connote a familial relationship. Drawing on recent fieldwork with members of entertaining troupes in Staffordshire and Greater Manchester, this article outlines some of the ways in which the ‘troupe family’ is invoked at troupe dancing events, including End of Season Championships and Family Night celebrations, as well as the crucial role played by troupe families in providing a shared context for rites of passage associated with the life cycle, such as births, deaths and marriages. I suggest that appeals to the troupe family reflect the high status placed on familial identities in the working-class communities of which troupe dancing is a part. Further, I argue that the family constitutes a transmission narrative in contradistinction to the use of ‘tradition’ in the cognate English folk dance community. This article considers the implications of the family model, and its affordances in safeguarding carnival performance while embracing change and renewal.

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