scholarly journals The focal complex of epithelial cells provides a signalling platform for interleukin-8 induction in response to bacterial pathogens

2014 ◽  
Vol 16 (9) ◽  
pp. 1441-1455 ◽  
Author(s):  
Tyson P. Eucker ◽  
Derrick R. Samuelson ◽  
Mary Hunzicker-Dunn ◽  
Michael E. Konkel
Keyword(s):  
Epithelial Cells ◽  
Bacterial Pathogens ◽  
Interleukin 8 ◽  
Focal Complex

N-acetyl- L-cysteine inhibits bleomycin-induced interleukin-8 secretion by bronchial epithelial cells

Respirology ◽  
2000 ◽  
Vol 5 (4) ◽  
pp. 309-313 ◽  
Author(s):  
Yasuhiro Gon ◽  
Shu Hashimoto ◽  
Tomoko Nakayama ◽  
Ken Matsumoto ◽  
Toshiya Koura ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Bronchial Epithelial Cells ◽  
Interleukin 8 ◽  
Bronchial Epithelial

scholarly journals Yersinia enterocolitica Adhesin A Induces Production of Interleukin-8 in Epithelial Cells

2004 ◽  
Vol 72 (12) ◽  
pp. 6780-6789 ◽  
Author(s):  
Yvonne Schmid ◽  
Guntram A. Grassl ◽  
Oliver T. Bühler ◽  
Mikael Skurnik ◽  
Ingo B. Autenrieth ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Yersinia Enterocolitica ◽  
Hela Cells ◽  
Map Kinases ◽  
Small Gtpases ◽  
Small Gtpase ◽  
Interleukin 8 ◽  
Β1 Integrin ◽  
Β1 Integrins ◽  
Tissue Reactions

ABSTRACT The major invasive factor of Yersinia enterocolitica, the invasin (Inv) protein, induces proinflammatory host cell responses, including interleukin-8 (IL-8) secretion from human epithelial cells, by engagement of β1 integrins. The Inv-triggered β1 integrin signaling involves the small GTPase Rac; the activation of MAP kinases, such as p38, MEK1, and JNK; and the activation of the transcription factor NF-κB. In the present study, we demonstrate that Y. enterocolitica YadA, which is a major adhesin of Y. enterocolitica with pleiotropic virulence effects, induces IL-8 secretion in epithelial cells. The abilites of YadA and Inv to promote adhesion to and invasion of HeLa cells and to induce IL-8 production by the cells were investigated by expression of YadA and Inv in Escherichia coli. While YadA mediates efficacious adhesion to HeLa cells, it mediates marginal invasion compared with Inv. Both YadA and Inv trigger comparable levels of IL-8 production. Conformational changes of the YadA head domain by mutation of NSVAIG-S motifs, which abolish collagen binding, also abolish adhesion of Yersinia to HeLa cells and YadA-mediated IL-8 secretion. Furthermore, experiments in which blocking antibodies against β1 integrins were used demonstrate that β1 integrins are crucial for YadA-mediated IL-8 secretion. Inhibitor studies demonstrate the involvement of small GTPases and MAP kinases, such as p38, MEK1, and JNK, indicating that β1 integrin-dependent signaling mediated by Inv or YadA involves similar signaling pathways. These data present YadA, in addition to Inv, YopB, and Yersinia lipopolysaccharide, as a further inducer of proinflammatory molecules by which Y. enterocolitica might promote inflammatory tissue reactions.

scholarly journals Black Tea Extract and Its Theaflavin Derivatives Inhibit the Growth of Periodontopathogens and Modulate Interleukin-8 and β-Defensin Secretion in Oral Epithelial Cells

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0143158 ◽  
Author(s):  
Telma Blanca Lombardo Bedran ◽  
Marie-Pierre Morin ◽  
Denise Palomari Spolidorio ◽  
Daniel Grenier
Keyword(s):  
Epithelial Cells ◽  
Black Tea ◽  
Interleukin 8 ◽  
Oral Epithelial Cells ◽  
Black Tea Extract ◽  
Tea Extract ◽  
Oral Epithelial

Inhibitory effect of carnosine on interleukin-8 production in intestinal epithelial cells through translational regulation

Cytokine ◽  
2008 ◽  
Vol 42 (2) ◽  
pp. 265-276 ◽  
Author(s):  
Dong Ok Son ◽  
Hideo Satsu ◽  
Yoshinobu Kiso ◽  
Mamoru Totsuka ◽  
Makoto Shimizu
Keyword(s):  
Epithelial Cells ◽  
Translational Regulation ◽  
Intestinal Epithelial Cells ◽  
Inhibitory Effect ◽  
Interleukin 8 ◽  
Intestinal Epithelial

Interleukin-8 expression by mammary gland endothelial and epithelial cells following experimental mastitis infection with E. coli

2006 ◽  
Vol 29 (2-3) ◽  
pp. 127-137 ◽  
Author(s):  
David McClenahan ◽  
Rebecca Krueger ◽  
Haa-Yung Lee ◽  
Chet Thomas ◽  
Marcus E. Kehrli ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Epithelial Cells ◽  
Interleukin 8 ◽  
E Coli

scholarly journals Blastocystis ratti Contains Cysteine Proteases That Mediate Interleukin-8 Response from Human Intestinal Epithelial Cells in an NF-κB-Dependent Manner

2007 ◽  
Vol 7 (3) ◽  
pp. 435-443 ◽  
Author(s):  
Manoj K. Puthia ◽  
Jia Lu ◽  
Kevin S. W. Tan
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Intestinal Inflammation ◽  
Experimental Studies ◽  
Cysteine Proteases ◽  
Interleukin 8 ◽  
Dependent Manner ◽  
Public Health Importance ◽  
Wide Range ◽  
First Time

ABSTRACT Blastocystis is a ubiquitous enteric protozoan found in the intestinal tracts of humans and a wide range of animals. Evidence accumulated over the last decade suggests association of Blastocystis with gastrointestinal disorders involving diarrhea, abdominal pain, constipation, nausea, and fatigue. Clinical and experimental studies have associated Blastocystis with intestinal inflammation, and it has been shown that Blastocystis has potential to modulate the host immune response. Blastocystis is also reported to be an opportunistic pathogen in immunosuppressed patients, especially those suffering from AIDS. However, nothing is known about the parasitic virulence factors and early events following host-parasite interactions. In the present study, we investigated the molecular mechanism by which Blastocystis activates interleukin-8 (IL-8) gene expression in human colonic epithelial T84 cells. We demonstrate for the first time that cysteine proteases of Blastocystis ratti WR1, a zoonotic isolate, can activate IL-8 gene expression in human colonic epithelial cells. Furthermore, we show that NF-κB activation is involved in the production of IL-8. In addition, our findings show that treatment with the antiprotozoal drug metronidazole can avert IL-8 production induced by B. ratti WR1. We also show for the first time that the central vacuole of Blastocystis may function as a reservoir for cysteine proteases. Our findings will contribute to an understanding of the pathobiology of a poorly studied parasite whose public health importance is increasingly recognized.

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