scholarly journals RON 12, a novel P lasmodium ‐specific rhoptry neck protein important for parasite proliferation

2013 ◽  
Vol 16 (5) ◽  
pp. 657-672 ◽  
Author(s):  
Ellen Knuepfer ◽  
Oniz Suleyman ◽  
Anton R. Dluzewski ◽  
Ursula Straschil ◽  
Aisling H. O'Keeffe ◽  
...  
Keyword(s):  
Rhoptry Neck Protein

scholarly journals Rhoptry neck protein RON2 forms a complex with microneme protein AMA1 in Plasmodium falciparum merozoites

2009 ◽  
Vol 58 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Jun Cao ◽  
Osamu Kaneko ◽  
Amporn Thongkukiatkul ◽  
Mayumi Tachibana ◽  
Hitoshi Otsuki ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Microneme Protein ◽  
Rhoptry Neck Protein

scholarly journals Rhoptry neck protein 11 has crucial roles during malaria parasite sporozoite invasion of salivary glands and hepatocytes

2019 ◽  
Vol 49 (9) ◽  
pp. 725-735 ◽  
Author(s):  
Sirasate Bantuchai ◽  
Mamoru Nozaki ◽  
Amporn Thongkukiatkul ◽  
Natcha Lorsuwannarat ◽  
Mayumi Tachibana ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Malaria Parasite ◽  
Rhoptry Neck Protein

Plasmodium falciparum rhoptry neck protein 5 peptides bind to human red blood cells and inhibit parasite invasion

Peptides ◽  
2014 ◽  
Vol 53 ◽  
pp. 210-217 ◽  
Author(s):  
Hernando Curtidor ◽  
Liliana C. Patiño ◽  
Gabriela Arévalo-Pinzón ◽  
Magnolia Vanegas ◽  
Manuel E. Patarroyo ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Parasite Invasion ◽  
Human Red Blood Cells ◽  
Rhoptry Neck Protein

scholarly journals Cross-reactivity between apical membrane antgen 1 and rhoptry neck protein 2 in P. vivax and P. falciparum: A structural and binding study

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0183198 ◽  
Author(s):  
Brigitte Vulliez-Le Normand ◽  
Frederick A. Saul ◽  
Sylviane Hoos ◽  
Bart W. Faber ◽  
Graham A. Bentley
Keyword(s):  
Apical Membrane ◽  
Cross Reactivity ◽  
Binding Study ◽  
Rhoptry Neck Protein

Plasmodial ortholog of Toxoplasma gondii rhoptry neck protein 3 is localized to the rhoptry body

2011 ◽  
Vol 60 (2) ◽  
pp. 132-138 ◽  
Author(s):  
Daisuke Ito ◽  
Eun-Taek Han ◽  
Satoru Takeo ◽  
Amporn Thongkukiatkul ◽  
Hitoshi Otsuki ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Rhoptry Neck Protein

scholarly journals Plasmodium falciparum AMA1 Binds a Rhoptry Neck Protein Homologous to TgRON4, a Component of the Moving Junction in Toxoplasma gondii

2006 ◽  
Vol 5 (7) ◽  
pp. 1169-1173 ◽  
Author(s):  
David L. Alexander ◽  
Shirin Arastu-Kapur ◽  
Jean-Francois Dubremetz ◽  
John C. Boothroyd
Keyword(s):  
Plasmodium Falciparum ◽  
Toxoplasma Gondii ◽  
Apical Membrane ◽  
Apical Membrane Antigen 1 ◽  
Apical Membrane Antigen ◽  
Rhoptry Neck Protein

ABSTRACT Plasmodium falciparum apical membrane antigen 1 (PfAMA1) coimmunoprecipitates with the Plasmodium homologue of TgRON4, a secreted rhoptry neck protein of Toxoplasma gondii that migrates at the moving junction in association with TgAMA1 during invasion. PfRON4 also originates in the rhoptry necks, suggesting that this unusual collaboration of micronemes and rhoptries is a conserved feature of Apicomplexa.

scholarly journals The rhoptry neck protein RON4 relocalizes at the moving junction during Toxoplasma gondii invasion

2005 ◽  
Vol 7 (12) ◽  
pp. 1823-1833 ◽  
Author(s):  
Maryse Lebrun ◽  
Adeline Michelin ◽  
Hiba El Hajj ◽  
Joel Poncet ◽  
Peter J. Bradley ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Rhoptry Neck Protein

scholarly journals Detection of the Rhoptry Neck Protein Complex in Plasmodium Sporozoites and Its Contribution to Sporozoite Invasion of Salivary Glands

mSphere ◽  
2020 ◽  
Vol 5 (4) ◽  
Author(s):  
Mamoru Nozaki ◽  
Minami Baba ◽  
Mayumi Tachibana ◽  
Naohito Tokunaga ◽  
Motomi Torii ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Protein Complex ◽  
Malaria Parasite ◽  
Gene Knockdown ◽  
Mammalian Host ◽  
Target Cells ◽  
Specific Gene ◽  
Invasion Mechanisms ◽  
Attachment Ability ◽  
Rhoptry Neck Protein

ABSTRACT In the Plasmodium life cycle, two infectious stages of parasites, merozoites and sporozoites, share rhoptry and microneme apical structures. A crucial step during merozoite invasion of erythrocytes is the discharge to the host cell membrane of some rhoptry neck proteins as a complex, followed by the formation of a moving junction involving the parasite-secreted protein AMA1 on the parasite membrane. Components of the merozoite rhoptry neck protein complex are also expressed in sporozoites, namely, RON2, RON4, and RON5, suggesting that invasion mechanism elements might be conserved between these infective stages. Recently, we demonstrated that RON2 is required for sporozoite invasion of mosquito salivary gland cells and mammalian hepatocytes, using a sporozoite stage-specific gene knockdown strategy in the rodent malaria parasite model, Plasmodium berghei. Here, we use a coimmunoprecipitation assay and oocyst-derived sporozoite extracts to demonstrate that RON2, RON4, and RON5 also form a complex in sporozoites. The sporozoite stage-specific gene knockdown strategy revealed that both RON4 and RON5 have crucial roles during sporozoite invasion of salivary glands, including a significantly reduced attachment ability required for the onset of gliding. Further analyses indicated that RON2 and RON4 reciprocally affect trafficking to rhoptries in developing sporozoites, while RON5 is independently transported. These findings indicate that the interaction between RON2 and RON4 contributes to their stability and trafficking to rhoptries, in addition to involvement in sporozoite attachment. IMPORTANCE Sporozoites are the motile infectious stage that mediates malaria parasite transmission from mosquitoes to the mammalian host. This study addresses the question whether the rhoptry neck protein complex forms and functions in sporozoites, in addition to its role in merozoites. By applying coimmunoprecipitation and sporozoite stage-specific gene knockdown assays, it was demonstrated that RON2, RON4, and RON5 form a complex and are involved in sporozoite invasion of salivary glands via their attachment ability. These findings shed light on the conserved invasion mechanisms among apicomplexan infective stages. In addition, the sporozoite stage-specific gene knockdown system has revealed for the first time in Plasmodium that the RON2 and RON4 interaction reciprocally affects their stability and trafficking to rhoptries. Our study raises the possibility that the RON complex functions during sporozoite maturation as well as migration toward and invasion of target cells.

Plasmodium falciparum: Genetic and immunogenic characterisation of the rhoptry neck protein PfRON4

2009 ◽  
Vol 122 (4) ◽  
pp. 280-288 ◽  
Author(s):  
Belinda J. Morahan ◽  
Georgina B. Sallmann ◽  
Robert Huestis ◽  
Valentina Dubljevic ◽  
Karena L. Waller
Keyword(s):  
Plasmodium Falciparum ◽  
Rhoptry Neck Protein
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