scholarly journals Effects of adenovirus expressing bone morphogenetic protein-4 on different cell types of osteoblastic differentiation

2018 ◽  
Vol 29 ◽  
pp. 119-119
Author(s):  
Yaeeun Rheu ◽  
In-Chul Rhyu ◽  
Young Ku ◽  
Yong-Moo Lee ◽  
Yang-Jo Seol ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Cell Types ◽  
Osteoblastic Differentiation ◽  
Bone Morphogenetic Protein 4 ◽  
Morphogenetic Protein ◽  
Different Cell Types

scholarly journals Ectopic transient overexpression of OCT-4 facilitates BMP4-induced osteogenic transdifferentiation of human umbilical vein endothelial cells

2020 ◽  
Vol 11 ◽  
pp. 204173142090920
Author(s):  
Seung Hyun L Kim ◽  
Seunghun S Lee ◽  
Inseon Kim ◽  
Janet Kwon ◽  
Song Kwon ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Cell Therapy ◽  
Bone Morphogenetic Protein ◽  
Umbilical Vein ◽  
Cell Types ◽  
Bone Morphogenetic Protein 4 ◽  
Morphogenetic Protein ◽  
Umbilical Vein Endothelial Cells

Limitation in cell sources for autologous cell therapy has been a recent focus in stem cell therapy and tissue engineering. Among various research advances, direct conversion, or transdifferentiation, is a notable and feasible strategy for the generation and acquirement of wanted cell source. So far, utilizing cell transdifferentiation technology in tissue engineering was mainly restricted at achieving single wanted cell type from diverse cell types with high efficiency. However, regeneration of a complete tissue always requires multiple cell types which poses an intrinsic complexity. In this study, enhanced osteogenic differentiation was achieved by transient ectopic expression of octamer-binding transcription factor 4 ( OCT-4) gene followed by bone morphogenetic protein 4 treatment on human umbilical vein endothelial cells. OCT-4 transfection and bone morphogenetic protein 4 treatment resulted in enhanced expression of osteogenic markers such as core-binding factor alpha 1, alkaline phosphatase, and collagen 1 compared with bone morphogenetic protein 4 treatment alone. Furthermore, we employed gelatin-heparin cryogel in cranial defect model for in vivo bone formation. Micro-computed tomography and histological analysis of in vivo samples showed that OCT-4 transfection followed by bone morphogenetic protein 4 treatment resulted in efficient transdifferentiation of endothelial cells to osteogenic cells. These results suggest that the combination of OCT-4 and bone morphogenetic protein 4 on endothelial cells would be a reliable multicellular transdifferentiation model which could be applied for bone tissue engineering.

Bone morphogenetic protein-4 (BMP-4) acts during gastrula stages to cause ventralization of Xenopus embryos

Development ◽  
1996 ◽  
Vol 122 (5) ◽  
pp. 1545-1554 ◽  
Author(s):  
C.M. Jones ◽  
L. Dale ◽  
B.L. Hogan ◽  
C.V. Wright ◽  
J.C. Smith
Keyword(s):  
Bone Morphogenetic Protein ◽  
Uv Irradiation ◽  
Cell Types ◽  
Bone Morphogenetic Protein 4 ◽  
Mesoderm Induction ◽  
Transcript Levels ◽  
Spemann's Organizer ◽  
Morphogenetic Protein ◽  
Initial Activation ◽  
Lines Of Evidence

Injection of RNA encoding BMP-4 into the early Xenopus embryo suppresses formation of dorsal and anterior cell types. To understand this phenomenon, it is necessary to know the stage at which BMP-4 acts. In this paper, we present three lines of evidence showing that BMP-4 misexpression has no effect on the initial steps of mesoderm induction, either dorsal or ventral, but instead causes ventralization during gastrulation. Firstly, activation of organizer-specific genes such as goosecoid, Xnot, pintallavis and noggin occurs normally in embryos injected with BMP-4 RNA, but transcript levels are then rapidly down-regulated as gastrulation proceeds. Similarly, BMP-4 does not affect the initial activation of goosecoid by activin in animal caps, but expression then declines precipitously. Secondly, embryos made ventral by injection with BMP-4 RNA cannot be rescued by grafts of Spemann's organizer at gastrula stages. Such embryos therefore differ from those made ventral by UV-irradiation, where the defect occurs early and rescue can be effected by the organizer. Finally, the dorsalizing effects of the organizer, and of the candidate dorsalizing signal noggin, both of which exert their effects during gastrulation, can be counteracted by BMP-4. Together, these experiments demonstrate that BMP-4 can act during gastrulation both to promote ventral mesoderm differentiation and to attenuate dorsalizing signals derived from the organizer.

scholarly journals Identification of bone morphogenetic protein 4 in the saliva after the placement of fixed orthodontic appliance

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Lovorka Grgurevic ◽  
Ruder Novak ◽  
Grgur Salai ◽  
Vladimir Trkulja ◽  
Lejla Ferhatovic Hamzic ◽  
...  
Keyword(s):  
Growth Factor ◽  
Bone Morphogenetic Protein ◽  
Bone Remodeling ◽  
Small Sample ◽  
Central Position ◽  
Published Data ◽  
Bone Morphogenetic Protein 4 ◽  
Orthodontic Appliance ◽  
Morphogenetic Protein ◽  
Fixed Orthodontic Appliance

Abstract Background This study was conducted in order to explore the effects of orthodontic tooth movement (OTM) on the changes of salivary proteome. This prospective observational pilot study recruited 12 healthy teenage boys with malocclusion treated with a fixed orthodontic appliance and 6 appropriate control participants. Saliva samples were collected a day before and at 0, 2, 7, and 30 days after initialization of treatment, corresponding to the initial, lag, and post-lag phases of OTM. Pooled samples were analyzed by liquid chromatography-mass spectrometry, ELISA, and Western blotting. To date, there is no published data on the presence of BMP molecules or their antagonists in the saliva or in the gingival cervical fluid related to orthodontic conditions. Results A total of 198 identified saliva proteins were classified based on their functional characteristics. Proteins involved in bone remodeling were observed exclusively 30 days post appliance placement, including bone morphogenetic protein 4 (BMP4), a BMP antagonist BMP-binding endothelial regulator, insulin-like growth factor-binding protein 3, cytoskeleton-associated protein 4, and fibroblast growth factor 5. Based on the analysis of protein interactions, BMP4 was found to have a central position in this OTM-related protein network. Conclusions The placement of a fixed orthodontic appliance induced occurrence of proteins involved in bone remodeling in the saliva at a time corresponding to the post-lag period of OTM. Limitations of this study include a relatively small sample size, limited time of monitoring patients, and the lack of interindividual variability assessment.

Sign in / Sign up

Export Citation Format

Share Document