Osseointegration of different implant surfaces in areas grafted with deproteinized bovine bone associated or not with fresh bone marrow ‐ Preclinical study in rabbits

2021 ◽  
Author(s):  
Amanda de Carvalho Silva Leocádio ◽  
Matusalém Silva Júnior ◽  
Guilherme José Pimentel Lopes de Oliveira ◽  
Élcio Marcantonio Júnior
Keyword(s):  
Bone Marrow ◽  
Preclinical Study ◽  
Bovine Bone ◽  
Fresh Bone ◽  
Deproteinized Bovine Bone

scholarly journals Application of Bone Morphogenetic Protein 7 Enhanced the Osteogenic Differentiation and Mineralization of Bone Marrow-Derived Stem Cells Cultured on Deproteinized Bovine Bone

Coatings ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 642
Author(s):  
Hyunjin Lee ◽  
Sae Kyung Min ◽  
Yoon-Hee Park ◽  
Jun-Beom Park
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Alkaline Phosphatase ◽  
Osteogenic Differentiation ◽  
Bovine Bone ◽  
Bone Morphogenetic Protein 7 ◽  
Bone Particle ◽  
Morphogenetic Protein ◽  
Cells Cultured ◽  
Deproteinized Bovine Bone

The growth of bone morphogenetic protein 7 (BMP-7) has been applied for tissue regeneration due to its osteoinductive properties. The aim of this research is to analyze the enhancing effects of BMP-7 on the osteogenic differentiation and mineralization of human bone marrow-derived stem cells cultured on the bovine bone particle. After the stem cells were loaded onto the bone graft material, their morphology was observed on day 7. Viability assays based on the application of fluorescent stains were used for qualitative analyses. Alkaline phosphatase activity assays and Alizarin red staining were used for the assessment of osteogenic differentiation on days 7 and 14. Next-generation mRNA sequencing was applied to evaluate global gene expression. Gene ontology and pathway analysis was used to propose the underlying mechanism. Fibroblast-like morphology was attained with the stem cells. The cells were shown to be firmly attached to the bone particle. Most of the stem cells produced an intense green fluorescence. The relative cellular viability assay values for BMP-7 groups at 0, 10, and 100 ng/mL on day 7 were 0.295 ± 0.003, 0.250 ± 0.002, and 0.240 ± 0.003, respectively (p < 0.05). Alkaline phosphatase activity was significantly higher in BMP-7 groups at concentration of 100 ng/mL compared to the control on days 7 and 14 (p < 0.05). The results of the mineralization assay showed significantly higher values for BMP-7 groups at 100 ng/mL concentration when compared with the control (p < 0.05). The expression of RUNX2 was increased with application of BMP-7 and mitogen-activated protein kinase pathway was associated with the target genes. Overall, this study shows that in vitro application of BMP-7 increases alkaline phosphorylase activity and mineralization of stem cells culture on deproteinized bovine bone mineral. The study suggests that combining stem cells with osteoinductive growth factors with scaffolds can have synergy effects on osteogenic differentiation.

scholarly journals DISTINCT EVENTS IN THE IMMUNE RESPONSE ELICITED BY TRANSFERRED MARROW AND THYMUS CELLS

1969 ◽  
Vol 130 (6) ◽  
pp. 1243-1261 ◽  
Author(s):  
G. M. Shearer ◽  
G. Cudkowicz
Keyword(s):  
Bone Marrow ◽  
Immune Response ◽  
Bone Marrow Cells ◽  
Second Step ◽  
Cell Divisions ◽  
Cellular Events ◽  
Antigen Complex ◽  
Fresh Bone ◽  
Thymus Cells ◽  
Marrow Cells

Marrow cells and thymocytes of unprimed donor mice were transplanted separately into X-irradiated syngeneic hosts, with or without sheep erythrocytes (SRBC). Antigen-dependent changes in number or function of potentially immunocompetent cells were assessed by retransplantation of thymus-derived cells with fresh bone marrow cells and SRBC; of marrow-derived cells with fresh thymocytes and SRBC; and of thymus-derived with marrow-derived cells and SRBC. Plaque-forming cells (PFC) of the direct (IgM) and indirect (IgG) classes were enumerated in spleens of secondary host mice at the time of peak responses. By using this two-step design, it was shown (a) that thymus, but not bone marrow, contained antigen-reactive cells (ARC) capable of initiating the immune response to SRBC (first step), and (b) that the same antigen complex that activated thymic ARC was required for the subsequent interaction between thymus-derived and marrow cells and/or for PFC production (second step). Thymic ARC separated from marrow cells but exposed to SRBC proliferated and generated specific inducer cells. These were the cells that interacted with marrow precursors of PFC to form the elementary units for plaque responses to SRBC, i.e. the class- and specificity-restricted antigen-sensitive units. It was estimated that each ARC generated 80–800 inducer cells in 4 days by way of a minimum of 6–10 cell divisions. On the basis of the available evidence, a simple model was outlined for cellular events in the immune response to SRBC.

Ridge preservation with the use of deproteinized bovine bone mineral

2013 ◽  
Vol 25 (7) ◽  
pp. 786-790 ◽  
Author(s):  
Jan Lindhe ◽  
Denis Cecchinato ◽  
Mauro Donati ◽  
Cristiano Tomasi ◽  
Birgitta Liljenberg
Keyword(s):  
Bone Mineral ◽  
Ridge Preservation ◽  
Bovine Bone ◽  
Bovine Bone Mineral ◽  
Deproteinized Bovine Bone Mineral ◽  
Deproteinized Bovine Bone

Deproteinized Bovine Bone Used as an Adjunct to Guided Bone Augmentation: An Experimental Study in the Rat

2001 ◽  
Vol 3 (3) ◽  
pp. 156-165 ◽  
Author(s):  
Andreas Stavropoulos ◽  
Lambros Kostopoulos ◽  
Nicolaos Mardas ◽  
Jens Randel Nyengaard ◽  
Thorkild Karring
Keyword(s):  
Experimental Study ◽  
Bovine Bone ◽  
Bone Augmentation ◽  
Deproteinized Bovine Bone

scholarly journals The Role of a Combination of Autogenous Fresh Bone Marrow and Omental Free Graft in the Cervical Esophageal Wound Healing in Dogs

2010 ◽  
Vol 5 (3) ◽  
pp. 202-207
Author(s):  
Omid Azari ◽  
Mohammad Mahdi Molaei ◽  
Reza Kheirandis ◽  
Sara Hamzeh Aliabad
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Free Graft ◽  
Fresh Bone

THE FATE OF BOVINE BONE MARROW STROMAL CELLS IN HYDROGELS

2008 ◽  
Vol 41 ◽  
pp. S130 ◽  
Author(s):  
Stephan Zeiter ◽  
Marije van der Werf ◽  
Keita Ito
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Bovine Bone
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