scholarly journals Physiological response of the retinal pigmented epithelium to 3‐ns pulse laser application, in vitro and in vivo

2021 ◽  
Author(s):  
John P. M. Wood ◽  
Marzieh Tahmasebi ◽  
Robert J. Casson ◽  
Malcolm Plunkett ◽  
Glyn Chidlow
Keyword(s):  
Pulse Laser ◽  
Physiological Response ◽  
Laser Application ◽  
Retinal Pigmented Epithelium ◽  
Pigmented Epithelium

scholarly journals Norbixin Protects Retinal Pigmented Epithelium Cells and Photoreceptors against A2E-Mediated Phototoxicity In Vitro and In Vivo

PLoS ONE ◽  
2016 ◽  
Vol 11 (12) ◽  
pp. e0167793 ◽  
Author(s):  
Valérie Fontaine ◽  
Elodie Monteiro ◽  
Elena Brazhnikova ◽  
Laëtitia Lesage ◽  
Christine Balducci ◽  
...  
Keyword(s):  
Retinal Pigmented Epithelium ◽  
Pigmented Epithelium ◽  
Epithelium Cells

Characterization of a spontaneously generated murine retinal pigmented epithelium cell line; a model for in vitro experiments

2016 ◽  
Vol 347 (2) ◽  
pp. 332-338
Author(s):  
Ehsan Ranaei Pirmardan ◽  
Zahra-Soheila Soheili ◽  
Shahram Samiei ◽  
Hamid Ahmadieh ◽  
Seyed Javad Mowla ◽  
...  
Keyword(s):  
Cell Line ◽  
In Vitro Experiments ◽  
Retinal Pigmented Epithelium ◽  
Epithelium Cell ◽  
Pigmented Epithelium

Cell adhesion and cytoskeleton in the retinal pigmented epithelium

1992 ◽  
Vol 50 (1) ◽  
pp. 484-485
Author(s):  
Michal Opas
Keyword(s):  
Cell Adhesion ◽  
Cell Movement ◽  
Retinal Pigmented Epithelium ◽  
Pigmented Epithelium ◽  
Development And Differentiation ◽  
And Control

The development and differentiation of tissues depend on the ability of cells to move and control their shape. In cell movement, a force generated by the contractile cytoplasmic machinery which includes the cytoskeletal microfilaments (MF) is, through cell-substratum adhesion, translated into traction. The chick embryonic retinal pigmented epithelium (RPE) differentiates in vitro, it can also be persuaded to transdifferentiate, and it displays differentiation dependent organization of the cytoskeleton, adhesiveness and ECM production.

Transfilter studies on the mechanism of epitheliomesenchymal interaction leading to chondrogenic differentiation of neural crest cells

Development ◽  
1983 ◽  
Vol 75 (1) ◽  
pp. 165-188
Author(s):  
Linda Smith ◽  
Peter Thorogood
Keyword(s):  
Neural Crest ◽  
Pore Size ◽  
Direct Contact ◽  
Chondrogenic Differentiation ◽  
Causal Mechanism ◽  
Pigmented Epithelium ◽  
Cell Processes ◽  
Scanning Electron

Interaction with an epithelium is a prerequisite for avian cranial neural crest (NC) cells to differentiate into cartilage and bone (Bee & Thorogood, 1980). In order to investigate the causal mechanism we have selected one such interaction - that between mesencephalic NC and retinal pigmented epithelium (RPE) for further study. Premigratory NC cells were grown transfilter to RPE explants of different developmental ages and on Nuclepore filters of different pore size which either allowed or prevented penetration by cell processes. Initial scanning electron microscopy (SEM) observations established that pores of 0·8 μm allowed the passage of cell processes through the filter whereas 0·2 μm pores did not. The transfilter experiments demonstrated that chondrogenic differentiation of NC cells will occur only if the Nuclepore filters have a pore size large enough to permit the passage of cell processes. Furthermore SEM observations established that cell processes do traverse the Nuclepore filter when NC and RPE are grown in transfilter combination. The results indicate that the mechanism is not mediated by diffusable factors but rather is mediated either by direct contact between NC cells and non-diffusable matrix closely associated with RPE or by direct plasmalemmal contact between RPE and NC cells through discontinuities in the basement membrane. The results of these experiments also demonstrate that younger (stage 17) RPE is more effective at eliciting chondrogenesis from premigratory NC cells than older (stage 24) RPE and that the interaction between RPE and NC cells is a prolonged one, taking place over days rather than within hours. Both of these in vitro observations are compatible with the timing of events leading to scleral cartilage formation in vivo.

scholarly journals Quail Pax-6 (Pax-QNR) mRNAs are expressed from two promoters used differentially during retina development and neuronal differentiation.

1995 ◽  
Vol 15 (6) ◽  
pp. 3344-3353 ◽  
Author(s):  
S Plaza ◽  
C Dozier ◽  
N Turque ◽  
S Saule
Keyword(s):  
Neuronal Differentiation ◽  
Untranslated Region ◽  
Primer Extension ◽  
Retinal Pigmented Epithelium ◽  
Rnase Protection ◽  
Retina Development ◽  
Myc Oncogene ◽  
Pigmented Epithelium ◽  
Alternate Promoters

During investigations on the regulation of the Pax-6 gene, we characterized a cDNA from quail neuroretina showing a 5' untranslated region distinct from that previously described and initiated from an internal promoter. Using RNase protection and primer extension mapping, we localized this second quail Pax-6 promoter, termed P1. As reported for the already described P0 promoter, P1 was also transactivated in vitro by the p46Pax-QNR protein. RNase protection assays performed with quail neuroretina RNA showed that P1-initiated mRNAs were detected before the P0-initiated mRNAs, remained constant up to embryonic day 8, and decreased slowly thereafter whereas, P0-initiated mRNAs accumulated up to embryonic day 8. In contrast, quail retinal pigmented epithelium expressed only the P1-initiated mRNAs. Transformation of these cells by the v-myc oncogene induced neuronal traits in the culture, which thereafter, in addition to the P1-initiated mRNAs, expressed Pax-QNR from the P0 promoter. These results suggest that expression of the quail Pax-6 gene is under the control of different regulators through alternate promoters, P0 being activated at the onset of neuronal differentiation.

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