Time-dependent morphological alterations and viability of cultured human trabecular cells after exposure to Trypan blue

2012 ◽  
Vol 41 (5) ◽  
pp. 484-490 ◽  
Author(s):  
Konstantinos T Tsaousis ◽  
Nikolaos Kopsachilis ◽  
Ioannis T Tsinopoulos ◽  
Stavros A Dimitrakos ◽  
Friedrich E Kruse ◽  
...  
Keyword(s):  
Trypan Blue ◽  
Time Dependent ◽  
Morphological Alterations ◽  
Trabecular Cells

Time-dependent Morphological Alterations Caused by Crimping of Artificial Heart Valves

2010 ◽  
Vol 143 (4) ◽  
pp. 331
Author(s):  
H. Aupperle ◽  
F. Gruenwald ◽  
P. Kiefer ◽  
J. Kempfert ◽  
T. Walther ◽  
...  
Keyword(s):  
Artificial Heart ◽  
Heart Valves ◽  
Time Dependent ◽  
Morphological Alterations ◽  
Artificial Heart Valves

scholarly journals P4-094: Time-dependent morphological alterations of dissolved synthesized amyloid-beta peptide

2006 ◽  
Vol 2 ◽  
pp. S541-S541
Author(s):  
Kazuyuki Machida ◽  
Atsuko Matsunaga ◽  
Masaharu Takumi ◽  
Yoshiharu Akitake ◽  
Kazuhiko Ono ◽  
...  
Keyword(s):  
Amyloid Beta ◽  
Time Dependent ◽  
Amyloid Beta Peptide ◽  
Morphological Alterations ◽  
Beta Peptide

scholarly journals Oxygen tension-independent protection against hypoxic cell killing in rat liver by low sodium

2017 ◽  
Author(s):  
Andrea Ferrigno ◽  
Laura G. Di Pasqua ◽  
Clarissa Berardo ◽  
Veronica Siciliano ◽  
Plinio Richelmi ◽  
...  
Keyword(s):  
Trypan Blue ◽  
Time Course ◽  
Time Dependent ◽  
Hypoxic Cell ◽  
Independent Manner ◽  
Hypoxic Injury ◽  
Low Sodium ◽  
Viable Cells ◽  
Homogenous Distribution

The role of Na+ in hypoxic injury was evaluated by a time-course analysis of damage in isolated livers perfused with N2-saturated buffer containing standard (143 mM) or low (25 mM) Na+ levels. Trypan blue uptake was used to detect non-viable cells. Under hypoxia with standard-Na+, trypan blue uptake began at the border between pericentral areas and periportal regions and increased in the latter zone; using a low-Na+ buffer, no trypan blue zonation occurred but a homogenous distribution of dye was found associated with sinusoidal endothelial cell (SEC) staining. A decrease in hyaluronic acid (HA) uptake, index of SEC damage, was observed using a low-Na+ buffer. A time dependent injury was confirmed by an increase in LDH and TBARS levels with standard-Na+ buffer. Using low-Na+ buffer, SEC susceptibility appears elevated under hypoxia and hepatocytes was protected, in an oxygen independent manner.

scholarly journals Chronic effect of vagotomy in the morphometry of the myenteric plexus of rats' duodenum

2010 ◽  
Vol 23 (3) ◽  
pp. 159-162
Author(s):  
Carlos Roberto Martins-Júnior ◽  
Aezio de Magalhães-Júnior ◽  
Paola Mayumi Inagaki ◽  
Pedro Paulo Pires ◽  
João José Lachat ◽  
...  
Keyword(s):  
Satellite Cells ◽  
Myenteric Plexus ◽  
Time Dependent ◽  
Test Results ◽  
Chronic Effect ◽  
Image System ◽  
Morphological Alterations ◽  
Post Surgery ◽  
Chronic Effects ◽  
Post Test

BACKGROUND: The gastrointestinal disorders have been associated with morphological alterations in the myenteric nervous plexus. AIM: To evaluate, through morphometric studies, the chronic effects of the subdiaphragmatic trunk vagotomy on the nervous plexus. METHODS: Fifteen male exemplars of Wistar Rattus novergicus weighing about 150g, distributed into three groups, have been used: control (n=5), Sham (n=5) and vagotomized (n=5). The animals were sacrificed after 30 and 90 days post surgery. Fragments of duodenum were fixed in Bouin solution, embedded into paraffin and stained with HE and PAS. Morphometric analysis was performed by a Carl Zeiss KM 450 image system. The following aspects were observed: the density of nervous cells per linear micrometer (µm) (ND); the area of perikarya (µm²) (NA); the number of satellite cells per µm (SCD); and the number of satellite cells per neuron (SC/N). The averages were compared with the help of "software" program Sigma Plus through two way - ANOVA and Tuckey post-test. RESULTS: Denervation increased SC/N (p<0,05) and NA (p<0,05), in a time-dependent denervation way (p<0,05). However ND and SCD, decreased, which significantly with the animal's age (p<0,001). CONCLUSION: Vagotomy altered the myenteric plexus morphology in a time-dependent way.

The Clinical, Roentgenological and Morphological Effects of an Acute Exposure of Phosgene on the Lungs of Dogs

1971 ◽  
Vol 29 ◽  
pp. 236-237
Author(s):  
R. F. Bils ◽  
W. F. Diller ◽  
F. Huth
Keyword(s):  
Latent Period ◽  
Chemical Industry ◽  
Toxic Substance ◽  
Lung Edema ◽  
X Rays ◽  
Beagle Dogs ◽  
Male And Female ◽  
Morphological Alterations ◽  
Before And After ◽  
Electron Microscopes

Phosgene still plays an important role as a toxic substance in the chemical industry. Thiess (1968) recently reported observations on numerous cases of phosgene poisoning. A serious difficulty in the clinical handling of phosgene poisoning cases is a relatively long latent period, up to 12 hours, with no obvious signs of severity. At about 12 hours heavy lung edema appears suddenly, however changes can be seen in routine X-rays taken after only a few hours' exposure (Diller et al., 1969). This study was undertaken to correlate these early changes seen by the roengenologist with morphological alterations in the lungs seen in the'light and electron microscopes.Forty-two adult male and female Beagle dogs were selected for these exposure experiments. Treated animals were exposed to 94.5-107-5 ppm phosgene for 10 min. in a 15 m3 chamber. Roentgenograms were made of the thorax of each animal before and after exposure, up to 24 hrs.

A Method for Electron Microscopic Study of Tissue Culture Cell Monolayers Grown in Tubes

1967 ◽  
Vol 25 ◽  
pp. 132-133
Author(s):  
R. Stephens ◽  
G. Schidlovsky ◽  
S. Kuzmic ◽  
P. Gaudreau
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Light Microscopy ◽  
Cell Sheet ◽  
Culture Cell ◽  
Tissue Culture Cell ◽  
Electron Microscopic ◽  
Cell Sheets ◽  
Morphological Alterations ◽  
Culture Cells

The usual method of scraping or trypsinization to detach tissue culture cell sheets from their glass substrate for further pelletization and processing for electron microscopy introduces objectionable morphological alterations. It is also impossible under these conditions to study a particular area or individual cell which have been preselected by light microscopy in the living state.Several schemes which obviate centrifugation and allow the embedding of nondetached tissue culture cells have been proposed. However, they all preserve only a small part of the cell sheet and make use of inverted gelatin capsules which are in this case difficult to handle.We have evolved and used over a period of several years a technique which allows the embedding of a complete cell sheet growing at the inner surface of a tissue culture roller tube. Observation of the same cell by light microscopy in the living and embedded states followed by electron microscopy is performed conveniently.

Sign in / Sign up

Export Citation Format

Share Document