scholarly journals Estrogen differentially regulates the expression of tyrosine hydroxylase and nerve growth factor through free radical generation in the thymus and mesenteric lymph nodes of middle-aged ovariectomized female Sprague-Dawley rats

2017 ◽  
Vol 8 (4) ◽  
pp. 341-350 ◽  
Author(s):  
Kishore A. Ravichandran ◽  
Sunil Karrunanithi ◽  
Lalgi Hima ◽  
Uday P. Pratap ◽  
Hannah P. Priyanka ◽  
...  
Keyword(s):  
Free Radical ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Tyrosine Hydroxylase ◽  
Lymph Nodes ◽  
Sprague Dawley ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Sprague Dawley Rats ◽  
Radical Generation

Effects of Inhaled Hexachlorobenzene Aerosols on Rat Pulmonary Host Defenses

1989 ◽  
Vol 5 (3) ◽  
pp. 451-461 ◽  
Author(s):  
Robert L. Sherwood ◽  
Peter T. Thomas ◽  
William J. O'shea ◽  
Jeannie N. Bradof ◽  
Helen V. Ratajczak ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
B Cell ◽  
Bactericidal Activity ◽  
Peritoneal Macrophages ◽  
Sprague Dawley ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Sprague Dawley Rats ◽  
Cell Mitogen

Pulmonary bactericidal activity, macrophage phagocytic activity, alveolar macrophage (AM) enzyme activity, and T- and B-cell mitogenesis of lymphocytes from lung associated lymph nodes (LALN) or mesenteric lymph nodes (MESLN) were assessed in Sprague-Dawley rats exposed 4 hr/d, 4 days/wk for 1, 4, or 16 days to hexachlorobenzene (HCB) aerosols. Pulmonary bactericidal activity was depressed after 1 or 4 but not 16 exposures to 35 mg/m3 of HCB. AM phagocytosis of 51Cr-RBC in vitro was increased after 4 but not 1 or 16 exposures to HCB, and no effect was observed in peritoneal macrophages. HCB significantly enhanced mitogenesis in MESLN to the B-cell mitogen Salmonella typhimurium lipopolysaccharide (STM) after 4 exposures; LALN STM mitogenesis and LALN and MESLN mitogenesis to phytohemagglutinin (PHA) were not affected. After 16 exposures, however, the PHA responses in LALN and MESLN were significantly increased and decreased, respectively.

scholarly journals Expression of nerve growth factor messenger RNA in pancreatic cancer tissues in Sprague Dawley rats

2005 ◽  
Vol 13 (18) ◽  
pp. 2227
Author(s):  
Zhu-Lin Yang ◽  
Xing-Hui Deng ◽  
Le-Ping Yang ◽  
Qing-Long Li ◽  
Wen-Tao Fan ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Messenger Rna ◽  
Sprague Dawley ◽  
Nerve Growth ◽  
Sprague Dawley Rats ◽  
Cancer Tissues

Inability of human clinical strains of Helicobacter pylori to colonize the alimentary tract of germfree rodents

1990 ◽  
Vol 36 (4) ◽  
pp. 237-241 ◽  
Author(s):  
Margherita T. Cantorna ◽  
Edward Balish
Keyword(s):  
Helicobacter Pylori ◽  
Lymph Nodes ◽  
Alimentary Tract ◽  
Sprague Dawley ◽  
Mesenteric Lymph Nodes ◽  
Immunodeficient Mice ◽  
Mesenteric Lymph ◽  
H Pylori ◽  
Rats And Mice ◽  
Culture Negative

Several attempts were made to colonize the alimentary tract and infect germfree BALB/c mice and germfree Sprague-Dawley rats with two human isolates of Helicobacter pylori. The alimentary tracts of mice, sacrificed at intervals between 1 day and 20 weeks after oral challenge, were culture negative for H. pylori. The alimentary tract, kidney, liver, and mesenteric lymph nodes were culture negative for H. pylori 5 h after intravenous challenge. Growth of H. pylori was inhibited by homogenates of murine stomach, small intestine, liver, and mesenteric lymph nodes. Germfree rats and mice do not appear to be readily colonized or infected by human strains of H. pylori. Key words: Helicobacter pylori, germfree mice, congenitally immunodeficient mice.

Abstract 444: Differential Contribution of Oxygenases in Glycerol-Induced Acute Renal Failure in the Rats

2015 ◽  
Vol 35 (suppl_1) ◽  
Author(s):  
Mohammad Newaz
Keyword(s):  
Oxidative Stress ◽  
Renal Failure ◽  
Acute Renal Failure ◽  
Free Radical ◽  
Disease Model ◽  
Oxidase Inhibitor ◽  
Sprague Dawley ◽  
Free Radical Generation ◽  
Sprague Dawley Rats ◽  
Radical Generation

Oxidative stress has long been identified as one if the mechanism in glycerol-induced acute renal failure (ARF), a disease model of human rhabdomyolysis. Besides NAD(P)H Oxidase, cyclooxygenase (COX) and xanthine oxidase (XO) are important oxygenase system that contributes in free radical generation in the biological system. In this study we explore involvement of these oxygenase system as the source of free radical in ARF and investigate the relationship between NAD(P)H oxidase, COX and XO in this process. Renal failure was induced in male Sprague Dawley rats by injecting glycerol (8 ml / kg; 50% v/v; i.m) with or without pretreatment of apocynin (Apo: mg/kg in drinking water, 7 days) a NAD(P)H Oxidase inhibitor. Rats were sacrificed 24 hrs after inducing ARF and kidney tissues were analyzed for biochemical assays. Glycerol increased free radical production by 39% as evident by elevated 8-isoprostane. Inhibition of NAD(P)H oxidase by apocynin reduced free radicals by 46%. In ARF rats, NAD(P)H oxidase, COX and XO activities were elevated by 106%, 70%, and 208%, respectively. Apocynin prevented this attenuation of NAD(P)H oxidase (54%; p<0.05) and COX (62%; p<0.05) but did not alter XO activity. These data suggests that NAD(P)H oxidase, COX, and XO are involved in generating oxidative stress in ARF. We also propose that COX-mediated free radical generation requires a functional NAD(P)H oxidase.

scholarly journals Retrobulbar administration of purified anti-nerve growth factor in developing rats induces structural and biochemical changes in the retina and cornea

2021 ◽  
Vol 14 (2) ◽  
pp. 209-216
Author(s):  
Luigi Aloe ◽  
◽  
Bijorn Omar Balzamino ◽  
Graziana Esposito ◽  
Alessandra Micera ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Protein Expression ◽  
Protein Profile ◽  
Quick Response ◽  
Sprague Dawley ◽  
Compensatory Mechanisms ◽  
Nerve Growth ◽  
Sprague Dawley Rats ◽  
Molecular Expression

AIM: To develop an experimental model of endogenous nerve growth factor (NGF) deprivation by retrobulbar administration of purified neutralizing anti-NGF antibodies in young Sprague-Dawley rats and provide further information on NGF expression in the retina and cornea. METHODS: Sixty old pathogen-free Sprague Dawley rats (p14, post-natal days) were treated with repeated retrobulbar injections of neutralizing anti-NGF (2 µL, 100 µg/mL, every 3d). After 2wk (p28), retinal and corneal tissues were investigated for morphological, biochemical, and molecular expression of trkANGFR by using Western blotting or immunofluorescence. Rhodopsin as well as protein profile expression were also investigated. RESULTS: Chronic retrobulbar neutralizing anti-NGF antibodies changed the distribution of trkANGFR immunoreactivity at retinal level, while no changes were detected for global trkANGFR protein expression. By contrary, the treatment resulted in the increase of corneal trkANGFR expression. Retinal tissues showed a decreased rhodopsin expression as well as reduced number of both rhodopsin expressing and total retinal cells, as observed after single cell extraction. A decreased expression of ICAM-1, IL-17 and IL-13 as well as an increased expression of IL-21 typified retinal extracts. No significant changes were observed for corneal tissues. CONCLUSION: The reduced availability of endogenous NGF, as produced by chronic retrobulbar anti-NGF administration, produce a quick response from retinal tissues, with respect to corneal ones, suggesting the presence of early compensatory mechanisms to protect retinal networking.

Effects of hyperoxia on rat diaphragm function

1994 ◽  
Vol 77 (1) ◽  
pp. 63-68 ◽  
Author(s):  
A. Anzueto ◽  
J. M. Brassard ◽  
F. H. Andrade ◽  
R. A. Lawrence ◽  
L. C. Maxwell ◽  
...  
Keyword(s):  
Free Radical ◽  
Sprague Dawley ◽  
Normobaric Hyperoxia ◽  
Diaphragm Function ◽  
Sprague Dawley Rats ◽  
Hyperoxia Exposure ◽  
Resistive Loading ◽  
Radical Generation ◽  
Air Control

The association of oxygen radical generation with impaired diaphragm performance has previously been reported after inspiratory resistive loading (IRL). We hypothesized that exposure of rats to normobaric hyperoxia (O2) could produce impaired diaphragm function because of free radical production. Sprague-Dawley rats were divided into four groups: 1) room air (control), 2) > 95% O2 for 24 h, 3) > 95% O2 for 48 h, and 4) > 95% O2 for 60 h. Each group was studied at rest after the O2 exposure and then after IRL. During IRL, the animals breathed through an inspiratory resistor until they were unable to sustain > 70% of the maximum airway pressure. Diaphragm samples were obtained for analysis of glutathione (GSH) and glutathione disulfide (GSSG) concentrations. In vitro isometric contractile properties were also determined, including maximal tetanic tension (Po) and maximal twitch tension (Pt), in GSSG content and in GSSG-to-GSH ratios. Hyperoxia for > 48 h resulted in significant decreases in Po and Pt and an increase in GSSG content and in GSSG-to-GSH ratios compared with other groups. Those same animals subjected to IRL showed a further decrease in Po and Pt. These data suggest that free radical generation may occur in the diaphragm during a hyperoxia exposure associated with activation of the GSH redox cycle and impairment of diaphragm function.

scholarly journals Effects of Monoclonal Antibodies against Nerve Growth Factor on Healthy Bone and Joint Tissues in Mice, Rats, and Monkeys: Histopathologic, Biomarker, and Microcomputed Tomographic Assessments

2018 ◽  
Vol 46 (4) ◽  
pp. 408-420 ◽  
Author(s):  
Kathryn E. Gropp ◽  
Cathy S. Carlson ◽  
Mark G. Evans ◽  
Cedo M. Bagi ◽  
William J. Reagan ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Sprague Dawley ◽  
Nerve Growth ◽  
Joint Replacements ◽  
Total Joint Replacements ◽  
Sprague Dawley Rats ◽  
Clinical Hold ◽  
High Doses ◽  
Rat Study

Tanezumab, an anti-nerve growth factor (NGF) antibody, is in development for management of chronic pain. During clinical trials of anti-NGF antibodies, some patients reported unexpected adverse events requiring total joint replacements, resulting in a partial clinical hold on all NGF inhibitors. Three nonclinical toxicology studies were conducted to evaluate the effects of tanezumab or the murine precursor muMab911 on selected bone and joint endpoints and biomarkers in cynomolgus monkeys, Sprague-Dawley rats, and C57BL/6 mice. Joint and bone endpoints included histology, immunohistochemistry, microcomputed tomography (mCT) imaging, and serum biomarkers of bone physiology. Responses of bone endpoints to tanezumab were evaluated in monkeys at 4 to 30 mg/kg/week for 26 weeks and in rats at 0.2 to 10 mg/kg twice weekly for 28 days. The effects of muMab911 at 10 mg/kg/week for 12 weeks on selected bone endpoints were determined in mice. Tanezumab and muMab911 had no adverse effects on any bone or joint parameter. There were no test article–related effects on bone or joint histology, immunohistochemistry, or structure. Reversible, higher osteocalcin concentrations occurred only in the rat study. No deleterious effects were observed in joints or bones in monkeys, rats, or mice administered high doses of tanezumab or muMab911.

scholarly journals Edema-producing activity of group A streptococcal polysaccharide and its possible role in the pathogenesis of cell wall-induced polyarthritis.

1983 ◽  
Vol 157 (4) ◽  
pp. 1089-1100 ◽  
Author(s):  
C Chetty ◽  
R R Brown ◽  
J H Schwab
Keyword(s):  
Cell Wall ◽  
Lymph Nodes ◽  
Sprague Dawley ◽  
Mesenteric Lymph Nodes ◽  
Sprague Dawley Rats ◽  
Specific Polysaccharide ◽  
Group A ◽  
Streptococcal Polysaccharide ◽  
Permeability Increase ◽  
Group A Streptococcal Polysaccharide

Edematous responses were induced in the limbs of Sprague-Dawley rats by intravenous, intraperitoneal, or intra-articular injections of group-specific polysaccharide (PS) isolated from the cell walls of group A streptococci. After intravenous injection of the edema-producing PS, vascular permeability increase (measured by 125I-human serum albumin) was detected in the limbs, but not in the heart, lungs, spleen, liver, thymus, kidney, skin, skeletal muscle, submandibular lymph nodes, mesenteric lymph nodes, or ascending colon. This indicates a selective effect on vascular endothelium of the joints. Evidence to suggest that the edema-producing activity of the PS might play an important role in the pathogenesis of cell wall-induced polyarthritis included the following: (a) the presence of edema-producing activity in arthropathogenic cell wall preparations; (b) cell wall preparations without edema-producing activity were significantly less active in inducing arthritis than were those which contained edema-producing activity; and (c) the addition of edema-producing PS to cell wall preparations increased both the incidence and the severity of arthritis.

In situ demonstration of migration of dendritic cells released from rat small intestine to mesenteric lymph nodes

2001 ◽  
Vol 120 (5) ◽  
pp. A183-A183
Author(s):  
H KOBAYASHI ◽  
H NAGATA ◽  
S MIURA ◽  
T AZUMA ◽  
H SUZUKI ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Small Intestine ◽  
Lymph Nodes ◽  
Rat Small Intestine ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph
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