Effects of tibolone or continuous combined oestradiol and norethisterone acetate on lipids, high‐density lipoprotein subfractions and apolipoproteins in postmenopausal women in a two‐year, randomized, double‐blind, placebo‐controlled trial

2020 ◽  
Vol 92 (4) ◽  
pp. 303-311
Author(s):  
Payal Trupti Kotecha ◽  
Ian F. Godsland ◽  
David Crook ◽  
John C. Stevenson
Keyword(s):  
High Density Lipoprotein ◽  
Postmenopausal Women ◽  
Controlled Trial ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Subfractions ◽  
Double Blind ◽  
Double Blind Placebo ◽  
High Density Lipoprotein Subfractions ◽  
Norethisterone Acetate

High-density lipoprotein subfractions measured in stored serum

1994 ◽  
Vol 40 (9) ◽  
pp. 1713-1716 ◽  
Author(s):  
L L Bausserman ◽  
A L Saritelli ◽  
D Milosavljevic
Keyword(s):  
High Density Lipoprotein ◽  
Dextran Sulfate ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
High Density ◽  
Manganese Chloride ◽  
Lipoprotein Subfractions ◽  
Hdl Subfractions ◽  
High Density Lipoprotein Subfractions

Abstract We compared the effects of freezing serum on the determination of high-density lipoprotein (HDL) subfractions by two dual-precipitation methods, heparin and manganese chloride/dextran sulfate (HM/DS) (Gidez et al., J Lipid Res 1982;23:1206-23) and DS/DS (Warnick et al., Clin Chem 1982;28:1574), and by ultracentrifugation. Storing serum for 1 month at -70 degrees C resulted in reduced HDL3-cholesterol by ultracentrifugation and reduced total and HDL3-cholesterol by the DS/DS method. There was no change in either total HDL-cholesterol or HDL3-cholesterol with the HM/DS method. Additional studies involving only HM/DS indicated that total HDL-cholesterol in serum stored at 4 degrees C begins to decline after 3 days (-3.1 +/- 3.5%, P < 0.1). HDL was stable at -20 degrees C for 2 weeks but both total and HDL3-cholesterol decreased significantly after 1 month. Storage of serum at -70 degrees C resulted in no changes for 1 year; however, at 18 months, HDL3-cholesterol was reduced 13% (P = 0.002). We conclude that HDL subfractions can be determined accurately in serum as well as in plasma after storage at -70 degrees C for up to 1 year.

scholarly journals High-Density Lipoprotein Subfractions - What the Clinicians Need to Know

Cardiology ◽  
2013 ◽  
Vol 124 (2) ◽  
pp. 116-125 ◽  
Author(s):  
Angela Pirillo ◽  
Giuseppe Danilo Norata ◽  
Alberico Luigi Catapano
Keyword(s):  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Subfractions ◽  
High Density Lipoprotein Subfractions
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