scholarly journals Monocytes show immunoregulatory capacity on CD4+ T cells in a human in-vitro model of extracorporeal photopheresis

2018 ◽  
Vol 195 (3) ◽  
pp. 369-380 ◽  
Author(s):  
F. Wiese ◽  
K. Reinhardt-Heller ◽  
M. Volz ◽  
C. Gille ◽  
N. Köstlin ◽  
...  
Keyword(s):  
T Cells ◽  
Cd4 T Cells ◽  
In Vitro Model ◽  
Extracorporeal Photopheresis ◽  
Vitro Model

scholarly journals Transcriptomic Analysis Implicates the p53 Signaling Pathway in the Establishment of HIV-1 Latency in Central Memory CD4 T Cells in an In Vitro Model

2016 ◽  
Vol 12 (11) ◽  
pp. e1006026 ◽  
Author(s):  
Cory H. White ◽  
Bastiaan Moesker ◽  
Nadejda Beliakova-Bethell ◽  
Laura J. Martins ◽  
Celsa A. Spina ◽  
...  
Keyword(s):  
T Cells ◽  
Signaling Pathway ◽  
Cd4 T Cells ◽  
In Vitro Model ◽  
P53 Signaling ◽  
Vitro Model ◽  
Memory Cd4 T Cells ◽  
P53 Signaling Pathway ◽  
Hiv 1

scholarly journals The Vitamin D reduces HIV-1 infection of CD4+ T cells in an in vitro model

2015 ◽  
Vol 6 ◽  
Author(s):  
Villegas-Ospina Simon ◽  
Aguilar-Jiménez Wbeimar ◽  
Gonzalez Sandra ◽  
Zapata Wildeman ◽  
Saulle Irma ◽  
...  
Keyword(s):  
Vitamin D ◽  
T Cells ◽  
Cd4 T Cells ◽  
In Vitro Model ◽  
Vitro Model ◽  
Hiv 1

scholarly journals Diminished HIV Infection of Target CD4+ T Cells in a Toll-Like Receptor 4 Stimulated in vitro Model

2019 ◽  
Vol 10 ◽  
Author(s):  
Ross Cromarty ◽  
Alex Sigal ◽  
Lenine J. P. Liebenberg ◽  
Lyle R. McKinnon ◽  
Salim S. Abdool Karim ◽  
...  
Keyword(s):  
T Cells ◽  
Hiv Infection ◽  
Cd4 T Cells ◽  
In Vitro Model ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Vitro Model

scholarly journals A new in vitro model of polymyositis reveals CD8+ T cell invasion into muscle cells and its cytotoxic role

Rheumatology ◽  
2019 ◽  
Vol 59 (1) ◽  
pp. 224-232
Author(s):  
Mari Kamiya ◽  
Fumitaka Mizoguchi ◽  
Akito Takamura ◽  
Naoki Kimura ◽  
Kimito Kawahata ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cd8 T Cells ◽  
Muscle Injury ◽  
In Vitro Model ◽  
Muscle Cells ◽  
Cd8 T Cell ◽  
Class I ◽  
Vitro Model

Abstract Objectives The hallmark histopathology of PM is the presence of CD8+ T cells in the non-necrotic muscle cells. The aim of this study was to clarify the pathological significance of CD8+ T cells in muscle cells. Methods C2C12 cells were transduced retrovirally with the genes encoding MHC class I (H2Kb) and SIINFEKL peptide derived from ovalbumin (OVA), and then differentiated to myotubes (H2KbOVA-myotubes). H2KbOVA-myotubes were co-cultured with OT-I CD8+ T cells derived from OVA-specific class I restricted T cell receptor transgenic mice as an in vitro model of PM to examine whether the CD8+ T cells invade into the myotubes and if the myotubes with the invasion are more prone to die than those without. Muscle biopsy samples from patients with PM were examined for the presence of CD8+ T cells in muscle cells. The clinical profiles were compared between the patients with and without CD8+ T cells in muscle cells. Results Analysis of the in vitro model of PM with confocal microscopy demonstrated the invasion of OT-I CD8+ T cells into H2KbOVA-myotubes. Transmission electron microscopic analysis revealed an electron-lucent area between the invaded CD8+ T cell and the cytoplasm of H2KbOVA-myotubes. The myotubes invaded with OT-I CD8+ T cells died earlier than the uninvaded myotubes. The level of serum creatinine kinase was higher in patients with CD8+ T cells in muscle cells than those without these cells. Conclusion CD8+ T cells invade into muscle cells and contribute to muscle injury in PM. Our in vitro model of PM is useful to examine the mechanisms underlying muscle injury induced by CD8+ T cells.

T Cells Support Osteoclastogenesis in an In Vitro Model Derived From Human Periodontitis Patients

2005 ◽  
Vol 76 (10) ◽  
pp. 1675-1680 ◽  
Author(s):  
G. Brunetti ◽  
S. Colucci ◽  
P. Pignataro ◽  
M. Coricciati ◽  
G. Mori ◽  
...  
Keyword(s):  
T Cells ◽  
In Vitro Model ◽  
Vitro Model

scholarly journals Lack of Coreceptor Allows Survival of Chronically Stimulated Double-Negative α/β T Cells

2001 ◽  
Vol 193 (10) ◽  
pp. 1113-1122 ◽  
Author(s):  
Abdel Rahim A. Hamad ◽  
Ananth Srikrishnan ◽  
Paria Mirmonsef ◽  
Chris P.M. Broeren ◽  
Carl H. June ◽  
...  
Keyword(s):  
T Cells ◽  
Cd4 T Cells ◽  
Cell Receptor ◽  
Double Negative ◽  
Cd4 Cells ◽  
Lymphoproliferative Diseases ◽  
Chronic Stimulation ◽  
Vitro Model ◽  
Massive Accumulation

Lymphoproliferative diseases are characterized by massive accumulation of CD4−CD8−B220+ (double-negative [DN]) T cells in peripheral organs. Although evidence indicates these cells are derived from mature autoreactive α/β T cells, the significance of coreceptor downregulation is not known. In this study, we examined the role CD4 coreceptor plays in the survival of repeatedly stimulated T cells. CD4+/+ and CD4−/− T cells from AND T cell receptor (TCR) transgenic mice exhibited similar phenotypes after antigenic stimulation, but the CD4−/− T cells survived in much larger numbers than the CD4+/+ cells upon primary and secondary major histocompatibility complex (MHC)/peptide stimulation. Enhanced survival of CD4−/− T cells was due to decreased apoptosis rather than enhanced proliferation. Similarly, circumvention of the CD4/MHC interaction by using a surrogate TCR ligand that does not engage CD4 led to significant enhancement of CD4+/+ cells than when stimulated with MHC/peptide. Finally, we generated DN B220+ T cells using an in vitro model system and showed they were more tolerant to chronic stimulation than CD4+/+ cells. Together, these results indicate that coreceptor engagement controls expansion of normal T cells. In the absence of coreceptor, T cells survive chronic stimulation and express B220 as seen in autoimmune lymphoproliferative diseases.

Induction of osteoclastogenesis in an in vitro model of Gaucher disease is mediated by T cells via TNF-α

Gene ◽  
2012 ◽  
Vol 509 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Juan M. Mucci ◽  
Romina Scian ◽  
Pablo N. De Francesco ◽  
Florencia Suqueli García ◽  
Romina Ceci ◽  
...  
Keyword(s):  
T Cells ◽  
Gaucher Disease ◽  
In Vitro Model ◽  
Tnf Α ◽  
Vitro Model
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