scholarly journals Human renal tubular epithelial cells suppress alloreactive T cell proliferation

2015 ◽  
Vol 179 (3) ◽  
pp. 509-519 ◽  
Author(s):  
M. W. H. J. Demmers ◽  
S. S. Korevaar ◽  
M. Roemeling-van Rhijn ◽  
T. P. P. van den Bosch ◽  
M. J. Hoogduijn ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
T Cell ◽  
Epithelial Cells ◽  
T Cell Proliferation ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Renal Tubular ◽  
Alloreactive T Cell

scholarly journals Renal tubular epithelial cells modulate T-cell responses via ICOS-L and B7-H1

2005 ◽  
Vol 68 (5) ◽  
pp. 2091-2102 ◽  
Author(s):  
Simone De Haij ◽  
Andrea M. Woltman ◽  
Leendert A. Trouw ◽  
Astrid C. Bakker ◽  
Sylvia W. Kamerling ◽  
...  
Keyword(s):  
T Cell ◽  
Epithelial Cells ◽  
T Cell Responses ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Cell Responses ◽  
Renal Tubular

scholarly journals Aldosterone Induces the Proliferation of Renal Tubular Epithelial Cells In Vivo but Not In Vitro

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Juan Hao ◽  
Lingjin Liu ◽  
Ziqian Liu ◽  
Gege Chen ◽  
Yunzhao Xiong ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Proliferation ◽  
Epithelial Cells ◽  
Mtt Assay ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Renal Tubular ◽  
Hk2 Cells

Objective. To investigate the proliferation effect of aldosterone on renal tubular epithelial cells in vivo and in vitro. Methods. Thirty-two male C57BL/6J mice (20–22 g) were divided randomly into four groups: sham, unilateral nephrectomy (UN), unilateral nephrectomy plus aldosterone infusion (UA), and UA plus eplerenone (UAE). The kidneys were removed 6 weeks after treatment. Expression of proliferating cell nuclear antigen (PCNA) was detected by immunohistochemistry and western blotting. Human kidney proximal tubular epithelial (HK2) and mouse distal convoluted tubule (mDCT) cell lines were stimulated by aldosterone (0, 10−9, 10−8, 10−7, and 10−6 mol/L) in vitro. Cells were collected after 3, 6, 12, 24, 36, and 48 h, and proliferation of each group detected by western blotting, flow cytometry, live imaging, and the MTT assay. In addition, mDCT cells were costimulated with a medium containing a final concentration of 161 mmol/L Na+ and different concentrations of aldosterone, and the number of cells and cellular DNA content was measured by the MTT assay and flow cytometry. Results. Aldosterone could induce a significant increase in the number of PCNA-positive cells in mouse kidneys accompanied by increased deposition of collagen fibers. Eplerenone could inhibit aldosterone-induced cell proliferation and collagen deposition. HK2 cells and mDCT cells administered different concentrations, and different times of aldosterone stimulation failed to cause cell proliferation, and costimulation of aldosterone and salt did not cause proliferation changes in mDCT cells. Conclusions. Aldosterone perfusion can induce proliferation of mouse kidney cells in vivo, and eplerenone can inhibit this change, but aldosterone stimulates HK2 cells and mDCT in vitro without causing their proliferation.

Auxin induces cell proliferation in an experimental model of mammalian renal tubular epithelial cells

Renal Failure ◽  
2015 ◽  
Vol 37 (5) ◽  
pp. 911-913 ◽  
Author(s):  
Valeria Cernaro ◽  
Maria Antonietta Medici ◽  
Giuseppa Leonello ◽  
Antoine Buemi ◽  
Franz Heinrich Kohnke ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cells ◽  
Experimental Model ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Renal Tubular

scholarly journals FP042AUXIN INDUCES CELL PROLIFERATION IN AN EXPERIMENTAL MODEL OF MAMMALIAN RENAL TUBULAR EPITHELIAL CELLS

2015 ◽  
Vol 30 (suppl_3) ◽  
pp. iii78-iii78
Author(s):  
Valeria Cernaro ◽  
Maria Antonietta Medici ◽  
Giuseppa Leonello ◽  
Antoine Buemi ◽  
Anna Maria Bruzzese ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cells ◽  
Experimental Model ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Renal Tubular
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