Anti-TWEAK monoclonal antibodies reduce vascular damage and leucocyte infiltration in a mouse model of cutaneous reverse passive Arthus reaction

2016 ◽  
Vol 41 (8) ◽  
pp. 871-877 ◽  
Author(s):  
T. Chen ◽  
Z.-P. Guo ◽  
L.-X. Fu ◽  
N. Cao ◽  
S. Qin
Keyword(s):  
Monoclonal Antibodies ◽  
Mouse Model ◽  
Vascular Damage ◽  
Arthus Reaction ◽  
Leucocyte Infiltration

Paeoniflorin suppresses vascular damage and the expression of E-selectin and ICAM-1 in a mouse model of cutaneous Arthus reaction

2013 ◽  
Vol 22 (7) ◽  
pp. 453-457 ◽  
Author(s):  
Tao Chen ◽  
Zai-pei Guo ◽  
Lin Wang ◽  
Sha Qin ◽  
Na Cao ◽  
...  
Keyword(s):  
Mouse Model ◽  
Vascular Damage ◽  
Arthus Reaction

scholarly journals Murine monoclonal antibodies against RBD of SARS-CoV-2 neutralize authentic wild type SARS-CoV-2 as well as B.1.1.7 and B.1.351 viruses and protect in vivo in a mouse model in a neutralization dependent manner

2021 ◽  
Author(s):  
Fatima Amanat ◽  
Shirin Strohmeier ◽  
Wen-Hsin Lee ◽  
Sandhya Bangaru ◽  
Andrew B Ward ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Severe Acute Respiratory Syndrome ◽  
Mouse Model ◽  
Neutralizing Antibodies ◽  
Dependent Manner ◽  
Receptor Binding Domain ◽  
Wild Type ◽  
Murine Monoclonal Antibodies

After first emerging in December 2019 in China, severe acute respiratory syndrome 2 (SARS-CoV-2) has since caused a pandemic leading to millions of infections and deaths worldwide. Vaccines have been developed and authorized but supply of these vaccines is currently limited. With new variants of the virus now emerging and spreading globally, it is essential to develop therapeutics that are broadly protective and bind conserved epitopes in the receptor binding domain (RBD) or the whole spike of SARS-CoV-2. In this study, we have generated mouse monoclonal antibodies (mAbs) against different epitopes on the RBD and assessed binding and neutralization against authentic SARS-CoV-2. We have demonstrated that antibodies with neutralizing activity, but not non-neutralizing antibodies, lower viral titers in the lungs when administered in a prophylactic setting in vivo in a mouse challenge model. In addition, most of the mAbs cross-neutralize the B.1.351 as well as the B.1.1.7 variants in vitro.

scholarly journals Isotype Switching Increases Efficacy of Antibody Protection against Cryptococcus neoformans Infection in Mice

1998 ◽  
Vol 66 (3) ◽  
pp. 1057-1062 ◽  
Author(s):  
Rui Rong Yuan ◽  
Gadi Spira ◽  
Jin Oh ◽  
Melia Paizi ◽  
Arturo Casadevall ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Mouse Model ◽  
Antibody Response ◽  
Cryptococcus Neoformans ◽  
Immunoglobulin G ◽  
Relative Efficacy ◽  
Isotype Switching ◽  
Mediate Immunity ◽  
Igg Isotypes ◽  
The Relationship

ABSTRACT The isotype and epitope specificities of antibodies both contribute to the efficacy of antibodies that mediate immunity toCryptococcus neoformans, but the relationship between these properties is only partially understood. In this study, we analyzed the efficacy of protection of two sets of immunoglobulin G (IgG) isotype switch variants from two IgG3 monoclonal antibodies (MAbs) which are either not protective or disease enhancing, depending on the mouse model used. The two IgG3 MAbs 3E5 and 4H3 have different epitope specificities. Protection experiments were done with A/JCr mice infected intravenously with C. neoformans and administered with 3E5 IgG3 and its IgG1, IgG2a, and IgG2b switch variants. These experiments revealed that IgG1, IgG2b, and IgG2a were each more effective than IgG3. For 4H3 IgG3 and its IgG1 and IgG2b switch variants, the relative efficacy was IgG2b > IgG1 >> IgG3. The combination of 3E5 IgG3 and 4H3 IgG3 was more deleterious than either IgG3 alone. All IgG isotypes were opsonic for mouse bronchoalveolar cells, with the relative efficacy being IgG2b > IgG2a > IgG1 > IgG3. These results (i) confirm that a nonprotective IgG3 MAb can be converted to a protective MAb by isotype switching, (ii) indicate that the efficacy of protection of an IgG1 MAb can be increased by isotype switching to another subclass, (iii) show that protective and nonprotective IgG MAbs are opsonic, and (iv) provide additional evidence for the concept that the efficacy of the antibody response to C. neoformans is dependent on the type of MAb elicited.

371: Investigation of Hypoxia in Renal Cell Carcinoma Using PET-CT Imaging of 1241-G250 Monoclonal Antibodies and Oxygen Tension Measurements in a Nude Mouse Model

2006 ◽  
Vol 175 (4S) ◽  
pp. 121-122
Author(s):  
Nathan Lawrentschuk ◽  
Angela Rigopoulos ◽  
Angela Mountain ◽  
Gareth Jones ◽  
Fook-Thean Lee ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Monoclonal Antibodies ◽  
Mouse Model ◽  
Cell Carcinoma ◽  
Oxygen Tension ◽  
Nude Mouse ◽  
Renal Cell ◽  
Ct Imaging ◽  
Nude Mouse Model ◽  
Pet Ct
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