scholarly journals Clinical and Immunological Evaluation of Cat‐allergic Asthmatics Living With or Without a Cat

2021 ◽  
Author(s):  
Erik R. Wambre ◽  
Mary Farrington ◽  
Veronique Bajzik ◽  
Hannah A. DeBerg ◽  
Marcella Ruddy ◽  
...  
Keyword(s):  
Immunological Evaluation

Production and immunological evaluation of epitope-based preventative pneumococcal candidate vaccine comprising immunodominant epitopes from PspA, CbpA, PhtD and PiuA antigens

2020 ◽  
Vol 22 ◽  
Author(s):  
Hesam Dorosti ◽  
Navid Nezafat ◽  
Reza Heidari ◽  
Mohammad Bagher Ghoshoon ◽  
Ahmad Gholami ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Pneumococcal Vaccine ◽  
Peptide Vaccine ◽  
Promising Alternative ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Serotype Replacement ◽  
Tnf Α ◽  
Immunological Evaluation ◽  
Immunodominant Epitopes

Background: Streptococcus pneumoniae is a leading cause of pneumonia, mostly in children less than five years and elderly people. Although the pneumoniae polysaccharide vaccine (PPV) and pneumonia conjugate vaccines (PCV) are the efficient pneumococcal vaccine in adult and children groups, but the serotype replacement of S. pneumoniae strains causes the reduction in the efficacy of PPV and PCV vaccines. Epitope-based vaccines are a promising alternative to the present capsular antigen vaccines. Methods: In this study, we evaluated cellular and humoral immune responses induced by our novel designed multi-epitope vaccine in BALB/c mice. CD8+ cytolytic T lymphocytes (CTLs) epitopes were selected from PspA and CbpA antigens, and CD4+ helper T lymphocytes (HTLs) epitopes were chosen from PhtD and PiuA antigens. PorB, the TLR2 agonist, as an adjuvant, was employed to increase the immunogenicity of the vaccine. Results and conclusion: The high levels of specific anti-peptide vaccine IgG and an increase in the level of IgG2 in the vaccinated group demonstrated our vaccine could elicit a robust antibody production. The significant increase in IFN-γ, IL-2, TNF-α, IL-4, IL-6, and decrease in IL-10 showed that, the designed vaccine could be proposed as the efficient preventative pneumococcal vaccine in the mouse model.

Immuno-compatibility of desaminotyrosine and desaminotyrosyl tyrosine functionalized star-shaped oligo(ethylene glycol)s with different molecular weights

2015 ◽  
Vol 1718 ◽  
pp. 97-102 ◽  
Author(s):  
Toralf Roch ◽  
Konstanze K. Julich-Gruner ◽  
Axel T. Neffe ◽  
Nan Ma ◽  
Andreas Lendlein
Keyword(s):  
Aqueous Solution ◽  
Ethylene Glycol ◽  
Average Molecular Weight ◽  
Molecular Weights ◽  
Immunological Effects ◽  
Microbial Products ◽  
Low Levels ◽  
Chain Lengths ◽  
Immunological Evaluation

ABSTRACTPolymer-based therapeutic strategies require biomaterials with properties and functions tailored to the demands of specific applications leading to an increasing number of newly designed polymers. For the evaluation of those new materials, comprehensive biocompatibility studies including cyto-, tissue-, and immunocompatibility are essential. Recently, it could be demonstrated that star-shaped amino oligo(ethylene glycol)s (sOEG) with a number average molecular weight of 5 kDa and functionalized with the phenol-derived moieties desaminotyrosine (DAT) or desaminotyrosyl tyrosine (DATT) behave in aqueous solution like surfactants without inducing a substantial cytotoxicity, which may qualify them as solubilizer for hydrophobic drugs in aqueous solution. However, for biomedical applications the polymer solutions need to be free of immunogenic contaminations, which could result from inadequate laboratory environment or contaminated starting material. Furthermore, the materials should not induce uncontrolled or undesired immunological effects arising from material intrinsic properties. Therefore, a comprehensive immunological evaluation as perquisite for application of each biomaterial batch is required. This study investigated the immunological properties of sOEG-DAT(T) solutions, which were prepared using sOEG with number average molecular weights of 5 kDa, 10 kDa, and 20 kDa allowing analyzing the influence of the sOEG chain lengths on innate immune mechanisms. A macrophage-based assay was used to first demonstrate that all DAT(T)-sOEG solutions are free of endotoxins and other microbial contaminations such as fungal products. In the next step, the capacity of the different DAT(T)-functionalized sOEG solutions to induce cytokine secretion and generation of reactive oxygen species (ROS) was investigated using whole human blood. It was observed that low levels of the pro-inflammatory cytokines interleukin(IL)-1β and IL-6 were detected for all sOEG solutions but only when used at concentrations above 250 µg·mL-1. Furthermore, only the 20 kDa sOEG-DAT induced low amounts of ROS-producing monocytes. Conclusively, the data indicate that the materials were not contaminated with microbial products and do not induce substantial immunological adverse effectsin vitro,which is a prerequisite for future biological applications.

Immunological evaluation of Escherichia coli -derived hepatitis C virus second envelope protein (E2) variants

2001 ◽  
Vol 58 (3) ◽  
pp. 221-228 ◽  
Author(s):  
S. Dueñas-Carrera ◽  
A. Viña ◽  
H.E. Garay ◽  
O. Reyes ◽  
L. Alvarez-Lajonchere ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Envelope Protein ◽  
Immunological Evaluation

Synthesis and immunological evaluation of N-acyl modified Globo H derivatives as anticancer vaccine candidates

2021 ◽  
Author(s):  
Canjia Zhai ◽  
Xiu-Jing Zheng ◽  
Cheng-Cheng Song ◽  
Xin-Shan Ye
Keyword(s):  
T Cell ◽  
Carbohydrate Antigen ◽  
Vaccine Candidates ◽  
Antitumor Vaccine ◽  
Cancer Immunotherapies ◽  
Anticancer Vaccine ◽  
Immunological Evaluation

Globo H is a tumor-associated carbohydrate antigen (TACA), which serves as a valuable target for antitumor vaccine or cancer immunotherapies. However, most TACAs are T-cell-independent and they cannot induce powerful...

scholarly journals Immunological Evaluation In Vitro of Nanoparticulate Impurities Isolated From Pharmaceutical-Grade Sucrose

2020 ◽  
Author(s):  
Adam Grabarek ◽  
Myriam Nabhan ◽  
Isabelle Turbica ◽  
Andrea Hawe ◽  
Marc Pallardy ◽  
...  
Keyword(s):  
Immunological Evaluation

scholarly journals Synthesis and immunological evaluation of synthetic peptide based anti-SARS-CoV-2 vaccine candidates

2021 ◽  
Author(s):  
Qingyu Zhao ◽  
Yanan Gao ◽  
Min Xiao ◽  
Xuefei Huang ◽  
Xuanjun Wu
Keyword(s):  
Synthetic Peptide ◽  
Spike Protein ◽  
Effective Vaccine ◽  
The Novel ◽  
Vaccine Candidates ◽  
Peptide Epitopes ◽  
Novel Coronavirus ◽  
Immunological Evaluation

For prevention of the coronavirus disease 2019 caused by the novel coronavirus SARS-CoV-2, an effective vaccine is critical. Herein, several potential peptide epitopes from the spike protein of SARS-CoV-2 have...

Immunological evaluation in oral lichen planus with chronic hepatitis C

1997 ◽  
Vol 32 (3) ◽  
pp. 324-329 ◽  
Author(s):  
Yumiko Nagao ◽  
Michio Sata ◽  
Kazuhiro Abe ◽  
Kyuichi Tanikawa ◽  
Tadamitsu Kameyama
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Chronic Hepatitis C ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Immunological Evaluation ◽  
Oral Lichen

Synthetic delivery system for tuberculosis vaccines: immunological evaluation of the M. tuberculosis 38 kDa protein entrapped in biodegradable PLG microparticles

Vaccine ◽  
1995 ◽  
Vol 13 (16) ◽  
pp. 1576-1582 ◽  
Author(s):  
H.M. Vordermeier ◽  
A.G.A. Coombes ◽  
P. Jenkins ◽  
J.P. McGee ◽  
D.T. O'Hagan ◽  
...  
Keyword(s):  
Delivery System ◽  
Immunological Evaluation
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