Detailed SAR and PCA of the Tyrocidines and Analogues Towards Leucocin A-Sensitive and Leucocin A-ResistantListeria monocytogenes

2014 ◽  
Vol 84 (5) ◽  
pp. 543-557 ◽  
Author(s):  
Adrienne Nyango-Nkeh Leussa ◽  
Marina Rautenbach
Keyword(s):  
Leucocin A

Manganese privation induced transcriptional upregulation of the class IIa bacteriocin plantaricin 423 in Lactobacillus plantarum 423

2021 ◽  
Author(s):  
Ross Vermeulen ◽  
Shelly Deane ◽  
Leon Dicks ◽  
Johann Rohwer ◽  
Anton Du Preez van Staden
Keyword(s):  
Transition Metal ◽  
Lactobacillus Plantarum ◽  
Foodborne Pathogens ◽  
Economic Value ◽  
Metal Homeostasis ◽  
Culture Conditions ◽  
Reporter System ◽  
Precursor Peptide ◽  
Enterocin A ◽  
Leucocin A

Plantaricin 423 is produced by Lactobacillus plantarum 423 using the pla biosynthetic operon located on the 8188 bp plasmid, pPLA4. As with many class IIa bacteriocin operons, the pla operon encodes biosynthetic genes ( plaA : precursor peptide, plaB : immunity, plaC : accessory and plaD : ABC transporter) but does not encode local regulatory genes. Little is known about the regulatory mechanisms involved in the expression of the apparently regulationless class IIa bacteriocins such as plantaricin 423. In this study, phylogenetic analysis of class IIa immunity proteins indicated that at least three distinct clades exist, which were then used to subgroup the class IIa operons. It became evident that the absence of classical quorum sensing genes on mobile bacteriocin encoding elements is a predisposition of the subgroup which includes plantaricin 423, pediocin AcH/PA-1, divercin V41, enterocin A, leucocin-A and -B, mesentericin Y105 and sakacin G. Further analysis of the subgroup suggested that the regulation of these class IIa operons may be linked to transition metal homeostasis in the host. By using a fluorescent promoter-reporter system in Lactobacillus plantarum 423, transcriptional regulation of plantaricin 423 was shown to be upregulated in response to manganese privation. IMPORTANCE Lactic acid bacteria hold huge industrial application and economic value, especially bacteriocinogenic strains which further aids in the exclusion of specific foodborne pathogens. Since bacteriocinogenic strains are sought after it is equally important to understand the mechanism of bacteriocin regulation. This is currently an understudied aspect of class IIa operons. Our research suggests the existence of a previously undescribed mode of class IIa bacteriocin regulation, whereby bacteriocin expression is linked to management of the producer’s transition metal homeostasis. This delocalized metalloregulatory model may fundamentally affect the selection of culture conditions for bacteriocin expression and change our understanding of class IIa bacteriocin gene transfer dynamics in a given microbiome.

scholarly journals Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum.

1991 ◽  
Vol 173 (23) ◽  
pp. 7491-7500 ◽  
Author(s):  
J W Hastings ◽  
M Sailer ◽  
K Johnson ◽  
K L Roy ◽  
J C Vederas ◽  
...  
Keyword(s):  
Leucocin A ◽  
Leuconostoc Gelidum ◽  
Bacteriocin Gene

scholarly journals Utilization of the leucocin A export system in Leuconostoc gelidum for production of a Lactobacillus bacteriocin

1995 ◽  
Vol 131 (1) ◽  
pp. 87-93 ◽  
Author(s):  
G.E. Allison ◽  
C. Ahn ◽  
M.E. Stiles ◽  
T.R. Klaenhammer
Keyword(s):  
Export System ◽  
Leucocin A ◽  
Leuconostoc Gelidum

scholarly journals Design, synthesis and evaluation of antimicrobial activity of N-terminal modified Leucocin A analogues

2013 ◽  
Vol 21 (13) ◽  
pp. 3715-3722 ◽  
Author(s):  
Krishna Chaitanya Bodapati ◽  
Rania Soudy ◽  
Hashem Etayash ◽  
Michael Stiles ◽  
Kamaljit Kaur
Keyword(s):  
Antimicrobial Activity ◽  
Design Synthesis ◽  
Leucocin A

Expression of mptC of Listeria monocytogenes induces sensitivity to class IIa bacteriocins in Lactococcus lactis

Microbiology ◽  
2004 ◽  
Vol 150 (8) ◽  
pp. 2663-2668 ◽  
Author(s):  
Manilduth Ramnath ◽  
Safia Arous ◽  
Anne Gravesen ◽  
John W. Hastings ◽  
Yann Héchard
Keyword(s):  
Lactic Acid ◽  
Listeria Monocytogenes ◽  
Lactic Acid Bacteria ◽  
Lactococcus Lactis ◽  
Inhibitory Activity ◽  
Bacteriocin Activity ◽  
Phosphotransferase System ◽  
Enterocin A ◽  
Leucocin A

Sensitivity to class IIa bacteriocins from lactic acid bacteria was recently associated with the mannose phosphotransferase system (PTS) permease, , in Listeria monocytogenes. To assess the involvement of this protein complex in class IIa bacteriocin activity, the mptACD operon, encoding , was heterologously expressed in an insensitive species, namely Lactococcus lactis, using the NICE double plasmid system. Upon induction of the cloned operon, the recombinant Lc. lactis became sensitive to leucocin A. Pediocin PA-1 and enterocin A also showed inhibitory activity against Lc. lactis cultures expressing mptACD. Furthermore, the role of the three genes of the mptACD operon was investigated. Derivative plasmids containing various combinations of these three genes were made from the parental mptACD plasmid by divergent PCR. The results showed that expression of mptC alone is sufficient to confer sensitivity to class IIa bacteriocins in Lc. lactis.

Surface-Conjugated Antimicrobial Peptide Leucocin A Displays High Binding to Pathogenic Gram-Positive Bacteria

2014 ◽  
Vol 6 (2) ◽  
pp. 1131-1138 ◽  
Author(s):  
Hashem Etayash ◽  
Lana Norman ◽  
Thomas Thundat ◽  
Michael Stiles ◽  
Kamaljit Kaur
Keyword(s):  
Antimicrobial Peptide ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
High Binding ◽  
Leucocin A
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