scholarly journals Prognostic impact of circulating tumor cells detected with the microfluidic “Universal CTC‐chip” for primary lung cancer

2021 ◽  
Author(s):  
Masatoshi Kanayama ◽  
Taiji Kuwata ◽  
Masataka Mori ◽  
Yukiko Nemoto ◽  
Natsumasa Nishizawa ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Primary Lung Cancer ◽  
Prognostic Impact

Circulating Tumor Cells in Pulmonary Venous Blood of Primary Lung Cancer Patients

2009 ◽  
Vol 87 (6) ◽  
pp. 1669-1675 ◽  
Author(s):  
Yoshitomo Okumura ◽  
Fumihiro Tanaka ◽  
Kazue Yoneda ◽  
Masaki Hashimoto ◽  
Teruhisa Takuwa ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Venous Blood ◽  
Primary Lung Cancer ◽  
Lung Cancer Patients

scholarly journals DNA methylome profiling of circulating tumor cells in lung cancer at single base-pair resolution

Oncogene ◽  
2021 ◽  
Author(s):  
Lei Zhao ◽  
Xiaohong Wu ◽  
Junnian Zheng ◽  
Dong Dong
Keyword(s):  
Lung Cancer ◽  
Dna Methylation ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Base Pair ◽  
Primary Lung Cancer ◽  
Mesenchymal Transition ◽  
Single Base ◽  
Dna Methylome ◽  
Single Base Pair

AbstractDNA methylation plays a pivotal role in regulating cellular processes, and altered DNA methylation pattern is a general hallmark of cancer. However, DNA methylome in circulating tumor cells (CTCs) is still a mystery due to the lack of proper analytical techniques. We introduced an efficient workflow, LCM–µWGBS, which can efficiently profile the DNA methylation of microdissected CTC samples. LCM–µWGBS combines the laser capture microdissection (LCM)-based CTC capture method and whole-genome bisulfite sequencing in very small CTC population (µWGBS) to gain insight into the DNA methylation landscape of CTCs. We herein profiled the DNA methylome of CTCs from lung cancer patients. Deriving from a comprehensive analysis of CTC methylome, a unique “CTC DNA methylation signature” that is distinct from primary lung cancer tissues was identified. Further analysis showed that promoter hypermethylation of epithelial genes is a hallmark of stable epithelial–mesenchymal transition process. Moreover, it has been suggested that CTCs are endowed with a stemness-related feature during dissemination and metastasis. This work constitutes a unique DNA methylation analysis of CTCs at single base-pair resolution, which might facilitate to propose noninvasive CTC DNA methylation biomarkers contributing to clinical diagnosis.

scholarly journals Prognostic Impact of Circulating Tumor Cells in Patients with Small Cell Lung Cancer

2012 ◽  
Vol 7 (3) ◽  
pp. 512-519 ◽  
Author(s):  
Tateaki Naito ◽  
Fumihiro Tanaka ◽  
Akira Ono ◽  
Kazue Yoneda ◽  
Toshiaki Takahashi ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Prognostic Impact ◽  
Small Cell Lung

The prognostic impact of circulating tumor cells in patients with small cell lung cancer.

2011 ◽  
Vol 29 (15_suppl) ◽  
pp. 10617-10617
Author(s):  
T. Naito ◽  
F. Tanaka ◽  
K. Yoneda ◽  
T. Takahashi ◽  
H. Murakami ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Prognostic Impact ◽  
Small Cell Lung

scholarly journals Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood

Cancers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 351 ◽  
Author(s):  
Galina Zamay ◽  
Olga Kolovskaya ◽  
Tatiana Ivanchenko ◽  
Tatiana Zamay ◽  
Dmitry Veprintsev ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Primary Lung Cancer ◽  
Lung Cancer Cells ◽  
Clinical Samples ◽  
Dna Aptamers ◽  
Microscopy Imaging ◽  
Lung Cancer Patients

We selected DNA aptamers to the epithelial cell adhesion molecule (EpCAM) expressed on primary lung cancer cells isolated from the tumors of patients with non-small cell lung cancer using competitive displacement of aptamers from EpCAM by a corresponding antibody. The resulting aptamers clones showed good nanomolar affinity to EpCAM-positive lung cancer cells. Confocal microscopy imaging and spectral profiling of lung cancer tissues confirmed the same protein target for the aptamers and anti-EpCAM antibodies. Furthermore, the resulted aptamers were successfully applied for isolation and detection of circulating tumor cells in clinical samples of peripheral blood of lung cancer patients.

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