scholarly journals High-throughput screening in colorectal cancer tissue-originated spheroids

2018 ◽  
Vol 110 (1) ◽  
pp. 345-355 ◽  
Author(s):  
Jumpei Kondo ◽  
Tomoya Ekawa ◽  
Hiroko Endo ◽  
Kanami Yamazaki ◽  
Norio Tanaka ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
High Throughput ◽  
High Throughput Screening ◽  
Cancer Tissue ◽  
Colorectal Cancer Tissue

scholarly journals O23: CHARACTERISING PATIENT-DERIVED COLORECTAL CANCER TISSUE-ORIGINATED ORGANOIDAL SPHEROIDS FOR HIGH-THROUGHPUT MICROFLUIDIC APPLICATIONS

2021 ◽  
Vol 108 (Supplement_1) ◽  
Author(s):  
MI Khot ◽  
M Levenstein ◽  
R Coppo ◽  
J Kondo ◽  
M Inoue ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fluid Flow ◽  
High Throughput ◽  
Microfluidic Devices ◽  
In Vitro Models ◽  
Fluorescent Imaging ◽  
Cancer Tissue ◽  
Cell Models

Abstract Introduction Three-dimensional (3D) cell models have gained reputation as better representations of in vivo cancers as compared to monolayered cultures. Recently, patient tumour tissue-derived organoids have advanced the scope of complex in vitro models, by allowing patient-specific tumour cultures to be generated for developing new medicines and patient-tailored treatments. Integrating 3D cell and organoid culturing into microfluidics, can streamline traditional protocols and allow complex and precise high-throughput experiments to be performed with ease. Method Patient-derived colorectal cancer tissue-originated organoidal spheroids (CTOS) cultures were acquired from Kyoto University, Japan. CTOS were cultured in Matrigel and stem-cell media. CTOS were treated with 5-fluorouracil and cytotoxicity evaluated via fluorescent imaging and ATP assay. CTOS were embedded, sectioned and subjected to H&E staining and immunofluorescence for ABCG2 and Ki67 proteins. HT29 colorectal cancer spheroids were produced on microfluidic devices using cell suspensions and subjected to 5-fluorouracil treatment via fluid flow. Cytotoxicity was evaluated through fluorescent imaging and LDH assay. Result 5-fluorouracil dose-dependent reduction in cell viability was observed in CTOS cultures (p<0.01). Colorectal CTOS cultures retained the histology, tissue architecture and protein expression of the colonic epithelial structure. Uniform 3D HT29 spheroids were generated in the microfluidic devices. 5-fluorouracil treatment of spheroids and cytotoxic analysis was achieved conveniently through fluid flow. Conclusion Patient-derived CTOS are better complex models of in vivo cancers than 3D cell models and can improve the clinical translation of novel treatments. Microfluidics can streamline high-throughput screening and reduce the practical difficulties of conventional organoid and 3D cell culturing. Take-home message Organoids are the most advanced in vitro models of clinical cancers. Microfluidics can streamline and improve traditional laboratory experiments.

scholarly journals HistoMosaic Detecting KRAS G12V Mutation Across Colorectal Cancer Tissue Slices through in Situ PCR

2016 ◽  
Vol 88 (5) ◽  
pp. 2792-2798 ◽  
Author(s):  
Christopher B. Raub ◽  
Chen-Chung Lee ◽  
Darryl Shibata ◽  
Clive Taylor ◽  
Emil Kartalov
Keyword(s):  
Colorectal Cancer ◽  
Cancer Tissue ◽  
Tissue Slices ◽  
In Situ Pcr ◽  
Colorectal Cancer Tissue

scholarly journals Tumor-infiltrating Immune Cells in H&E-stained Sections of Colorectal Cancer Tissue as a Reasonable Immunological Biomarker

2018 ◽  
Vol 38 (12) ◽  
pp. 6721-6727 ◽  
Author(s):  
SHINJI MATSUTANI ◽  
MASATSUNE SHIBUTANI ◽  
KIYOSHI MAEDA ◽  
HISASHI NAGAHARA ◽  
TATSUNARI FUKUOKA ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Immune Cells ◽  
Cancer Tissue ◽  
Colorectal Cancer Tissue

The stability of dihydropyrimidine dehydrogenase activity in colorectal cancer tissue after resection

2003 ◽  
Author(s):  
Sotaro Sadahiro ◽  
Toshiyuki Suzuki ◽  
Kenji Ishikawa ◽  
Tomoki Nakamura ◽  
Akemi Kamijo ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dehydrogenase Activity ◽  
Dihydropyrimidine Dehydrogenase ◽  
Cancer Tissue ◽  
Colorectal Cancer Tissue ◽  
The Stability

scholarly journals The Effects of Storage Time of Colorectal Cancer Tissue on the Detection of K-rasGene

2011 ◽  
Vol 42 (8) ◽  
pp. 478-481 ◽  
Author(s):  
Yong-qi Shen ◽  
Xian-bin Feng ◽  
Yong-rong Lai ◽  
Xue-jian Ning ◽  
Xian-ji Fan ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Storage Time ◽  
Cancer Tissue ◽  
Colorectal Cancer Tissue
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