scholarly journals Blockade of ONECUT2 expression in ovarian cancer inhibited tumor cell proliferation, migration, invasion and angiogenesis

2018 ◽  
Vol 109 (7) ◽  
pp. 2221-2234 ◽  
Author(s):  
Tongyi Lu ◽  
Binhua Wu ◽  
Yunfei Yu ◽  
Wenhui Zhu ◽  
Simin Zhang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Proliferation ◽  
Tumor Cell ◽  
Tumor Cell Proliferation

Bipterostilbene, a Novel Compound From Rhubarb, Specifically Inhibits the Proliferation of Malignant B Cells Via Inactivation of AKT/mTOR Signaling Pathway

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 1423-1423
Author(s):  
You Hua Yu ◽  
Na Guo ◽  
Yujing Gong ◽  
Baidong Liu ◽  
Hong Liu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ovarian Cancer ◽  
Cell Proliferation ◽  
Tumor Cell ◽  
B Cell ◽  
Signaling Pathway ◽  
Cell Lines ◽  
Tumor Cell Proliferation ◽  
Mtor Signaling Pathway ◽  
B Cell Malignancies

Abstract Abstract 1423 Patients with B cell malignaces initially respond to current treatment modalities, however, such malignances remain incurable. Many new therapeutic options have become available during the past several years but nearly all patients develop resistance to currently available therapeutic options. Ideally, a new treatment should inhibit tumor growth, improve the efficacy of other anti-tumor agents, and improve both the overal survial and the quality of life for patients. Pterostilbene is predominantly found in Rhubarb. We synthesized bipterostilbene (5-(4-(4-(3,5-dihydroxylstyryl)phenoxy)styryl)-benzene-1,3-diol) (C28H22O5) of a molecular weight of 438.48 Kda. In this study, we first examined whether bipterostilbene affects tumor cells proliferation using breast cancer, ovarian cancer, lymphoma and multiple myeloma (MM) cell lines. The results of the MTS assay demonstrated that bipterostilbene significantly inhibited tumor cell proliferation of the lymphoma cell line (Raji) and the MM cell lines (RPMI1640 and MM1s) at 48 hours (IC50: 5μM for Raji, 4μM for RPMI8226, and 2 μM for MM1s). The induction of tumor cell apoptosis was most prominent at 72 hours. The extent of the inhibition of tumor cell proliferation and the induction of apoptosis was concentration-dependent. Bipterostilbene had minimal effects on breast and ovarian cancer cell lines. Noteworthy, bipterostilbene had no detectable cytotoxic effects on normal human peripheral blood mononuclear cells (PBMCs). The molecular mechanism by which bipterostilbene mediates its effects was examined. Both the AKT and the NF-κB signaling transduction pathways have been reported to play key roles in B cell metabolism, proliferation and survival. Using RT-PCR, bipterostilbene specifically inhibited AKT1 and mTOR gene expression when Raji or RPMI8226 tumor cells were treated with the IC50 concentration of bipterostilbene for 24 hours. Analysis of downstream gene products of the AKT pathway revealed that Cyclin D1 expression was slightly reduced and P21Cip and P27 kip expressions were not changed. Bipterostilbene did not alter AKT2 or AKT3 gene expression, demonstrating that this compound is specifically targeting AKT1. We further determined whether bipterostilbene interfered with IGF1-induced AKT/mTOR activation or IL-1β –mediated NF-κB phosphorylation by Western blot. The results showed that bipterostilbene markedly inhibited IGF1-induced phosphorylation of AKT but did not interfere with IL-1β-induced NF-κB activity and IκB phosphorylation. Overall, the results of our in vitro studies demonstrate that bipterostilbene inhibits tumor cell proliferation and enhances apoptosis of B-cell malignancies via inhibition of the AKT/mTOR signaling pathway with no detectable effect on the NF-κB signaling pathway. Importantly, bipterostilbene is not cytotoxic on normal hematopoietic cells at concentrations that were highly toxic to B-cell malignancies. We propose that bipterostilbene may be better tolerated than other anti- cancer drugs that are currently being used for the treatment of B-cell malignancies. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Sam68 is Overexpressed in Epithelial Ovarian Cancer and Promotes Tumor Cell Proliferation

2016 ◽  
Vol 22 ◽  
pp. 3248-3256 ◽  
Author(s):  
Lijuan Dong ◽  
Hailuo Che ◽  
Mingmei Li ◽  
Xuepeng Li
Keyword(s):  
Ovarian Cancer ◽  
Cell Proliferation ◽  
Tumor Cell ◽  
Epithelial Ovarian Cancer ◽  
Tumor Cell Proliferation

scholarly journals LRH1 promotes tumor cell proliferation and migration, and is correlated with poor prognosis in ovarian cancer

2020 ◽  
Author(s):  
Wenzhou Sun ◽  
Qingtao Shi ◽  
Jiaxin Li ◽  
Jinmeng Li ◽  
Libo Yu
Keyword(s):  
Ovarian Cancer ◽  
Cell Proliferation ◽  
Tumor Cell ◽  
Ubiquitin Ligase ◽  
Peritoneal Metastasis ◽  
Bioinformatics Analysis ◽  
Figo Stage ◽  
Tumor Cell Proliferation ◽  
Ubiquitin Ligase Complex

Abstract Background Liver receptor homolog 1 (LRH1) plays a vital role in several human cancers, but its role in ovarian cancer (OC) remains unclear. We aimed to explore the functions of LRH1 and its clinical relevance. Methods LRH1 expression was evaluated by immunohistochemistry and reverse transcription quantitative polymerase chain reaction (RT-qPCR). The effects of LRH1 on tumor cell proliferation, migration and epithelial-mesenchymal transition (EMT) were evaluated in vitro. Furthermore, bioinformatics analysis was applied to predict the functions of LRH1. Results RT-qPCR showed that LRH1 mRNA expression was intense in the invasive lesions (P < 0.05). LRH1 overexpression was extremely related with elevated International Federation of Gynecology and Obstetrics (FIGO) stage (P = 0.001), lymph node metastasis (P = 0.011), and peritoneal metastasis (P = 0.001). Furthermore, LRH1 expression was an independent prognostic index for disease-free survival in patients with OC (P = 0.041). LRH1 overexpression (P=0.011), FIGO stage (P<0.001), and ascites (P=0.015) independently affected peritoneal metastasis in patients with OC. LRH1 knockdown significantly inhibited the proliferation, migration, and EMT of human OC cells (P < 0.05). Bioinformatics analysis indicated that the functions of LRH1 were associated with the PRC1 complex, nuclear ubiquitin ligase complex, and Polycomb-group (PcG) proteins. Conclusions This study provides evidence of the predictive value of LRH1 on peritoneal metastasis and poor outcome and highlights the potential role of LRH1 as a biomarker for the targeted therapy of OC. Furthermore, LRH1 promotes OC cell proliferation, migration, and EMT in vitro, and its functions may be associated with PRC1 complex, nuclear ubiquitin ligase complex, and PcG proteins.

Eukaryotic elongation factors 2 promotes tumor cell proliferation and correlates with poor prognosis in ovarian cancer

2018 ◽  
Vol 53 ◽  
pp. 53-60 ◽  
Author(s):  
Nannan Shi ◽  
Xiaojing Chen ◽  
Rong Liu ◽  
Danping Wang ◽  
Min Su ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Proliferation ◽  
Tumor Cell ◽  
Poor Prognosis ◽  
Tumor Cell Proliferation ◽  
Elongation Factors

scholarly journals LRH1 promotes tumor cell proliferation, migration and correlates with poor prognosis in ovarian cancer

2020 ◽  
Author(s):  
Wenzhou Sun ◽  
Qingtao Shi ◽  
Jiaxin Li ◽  
Jinmeng Li ◽  
Libo Yu
Keyword(s):  
Ovarian Cancer ◽  
Cell Proliferation ◽  
Tumor Cell ◽  
Peritoneal Metastasis ◽  
Prognostic Index ◽  
Disease Free Survival ◽  
Figo Stage ◽  
Vital Role ◽  
Tumor Cell Proliferation

Abstract Background Recent studies have revealed that liver receptor homolog 1 (LRH1) plays a vital role in several human cancers, but its role in ovarian cancer (OC) remains unclear. We aimed to investigate whether LRH1 protein can act as a promising biomarker to predict the prognosis and metastasis in OC patients.Methods LRH1 expression status was evaluated by immunohistochemical and reverse transcription (RT)-qPCR analysis. The effects of LRH1 on tumor cell proliferation and progression were evaluated in vitro. Results RT-qPCR showed that LRH1 mRNA expression was more intense in the invasive lesions (P < 0.05). LRH1 overexpression were extremely related with elevated FIGO stage (P = 0.001), lymph node metastasis (P = 0.011), and peritoneal metastasis (P = 0.001). Furthermore, LRH1 expression was an independently prognostic index for disease-free survival in OC patients (P = 0.041). In addition, LRH1 overexpression (P=0.011) along with FIGO stage (P<0.001) and ascites (P=0.015) independently affect peritoneal metastasis in OC patients. LRH1 knockdown significantly inhibited the proliferation, migration, and metastasis of human OC cells (P < 0.05).Conclusions The present study provides evidence that LRH1 predicts peritoneal metastasis and poor outcome, highlighting its potential role as biomarker for targeted therapy in OC.

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