scholarly journals Amino-terminal enhancer of split gene AES encodes a tumor and metastasis suppressor of prostate cancer

2017 ◽  
Vol 108 (4) ◽  
pp. 744-752 ◽  
Author(s):  
Yoshiyuki Okada ◽  
Masahiro Sonoshita ◽  
Fumihiko Kakizaki ◽  
Naoki Aoyama ◽  
Yoshiro Itatani ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Metastasis Suppressor ◽  
Amino Terminal ◽  
Split Gene ◽  
Enhancer Of Split

287 Amino-terminal enhancer of split/AES is a tumor and metastasis suppressor of prostate cancer

2015 ◽  
Vol 14 (2) ◽  
pp. e287-e287a
Author(s):  
Y. Okada ◽  
T. Kobayashi ◽  
Y. Makino ◽  
M. Uegaki ◽  
Y. Miyazaki ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Metastasis Suppressor ◽  
Amino Terminal ◽  
Enhancer Of Split

MP41-08 AMINO-TERMINAL ENHANCER OF SPLIT/ AES IS A TUMOR AND METASTASIS SUPPRESSOR OF PROSTATE CANCER

2014 ◽  
Vol 191 (4S) ◽  
Author(s):  
Yoshiyuki Okada ◽  
Fumihiko Kakizaki ◽  
Masayuki Uegaki ◽  
Takayuki Goto ◽  
Takeshi Yoshikawa ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Metastasis Suppressor ◽  
Amino Terminal ◽  
Enhancer Of Split

scholarly journals Proteolytic cleavage and truncation of NDRG1 in human prostate cancer cells, but not normal prostate epithelial cells

2013 ◽  
Vol 33 (3) ◽  
Author(s):  
Mohammad K. Ghalayini ◽  
Qihan Dong ◽  
Des R. Richardson ◽  
Stephen J. Assinder
Keyword(s):  
Prostate Cancer ◽  
Epithelial Cells ◽  
Western Blot ◽  
Cancer Cells ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Metastasis Suppressor ◽  
Normal Prostate ◽  
Truncated Protein ◽  
Prostate Epithelial Cells

NDRG1 (N-myc downstream regulated gene-1) is a metastasis suppressor that is down-regulated in prostate cancer. NDRG1 phosphorylation is associated with inhibition of metastasis and Western blots indicate two bands at ~41 and ~46 kDa. Previous investigations by others suggest the higher band is due to NDRG1 phosphorylation. However, the current study using a dephosphorylation assay and the Phos-tag (phosphate-binding tag) SDS/PAGE assay, demonstrated that the 46 kDa NDRG1 protein band was not due to phosphorylation. Further experiments showed that the NDRG1 protein bands were not affected upon glycosidase treatment, despite marked effects of these enzymes on the glycosylated protein, fetuin. Analysis using RT–PCR (reverse transcriptase–PCR) demonstrated only a single amplicon, and thus, the two bands could not result from an alternatively spliced NDRG1 transcript. Western-blot analysis of prostate cancer cell lysates identified the 41 kDa band to be a truncated form of NDRG1, with MS confirming the full and truncated proteins to be NDRG1. Significantly, this truncated protein was not present in normal human PrECs (prostate epithelial cells). Western-blot analysis using anti-NDRG1 raised to its N-terminal sequence failed to detect the truncated protein, suggesting that it lacked N-terminus amino acids (residues 1–49). Sequence analysis predicted a pseudotrypsin protease cleavage site between Cys49–Gly50. Such cleavage of NDRG1 in cancer cells may result in loss of NDRG1 tumour suppressive activity.

scholarly journals The Amino-terminal Peptide Of Bax Perturbs Intracellular Ca2+ Homeostasis To Enhance Apoptosis In Prostate Cancer Cells

2009 ◽  
Vol 96 (3) ◽  
pp. 424a
Author(s):  
Na Li ◽  
Peihui Lin ◽  
Chuanxi Cai ◽  
Zui Pan ◽  
Noah Weisleder ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Prostate Cancer Cells ◽  
Amino Terminal

scholarly journals Human homolog of Drosophila Hairy and enhancer of split 1, Hes1, negatively regulates δ-catenin (CTNND2) expression in cooperation with E2F1 in prostate cancer

2010 ◽  
Vol 9 (1) ◽  
pp. 304 ◽  
Author(s):  
Jian-Ping Lu ◽  
Jiao Zhang ◽  
Kwonseop Kim ◽  
Thomas C Case ◽  
Robert J Matusik ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Human Homolog ◽  
Enhancer Of Split
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