scholarly journals Role of micro RNA in the pathogenesis of malignant lymphoma

2013 ◽  
Vol 104 (7) ◽  
pp. 801-809 ◽  
Author(s):  
Hiroyuki Tagawa ◽  
Sho Ikeda ◽  
Kenichi Sawada
Keyword(s):  
Malignant Lymphoma ◽  
Micro Rna

scholarly journals Characterization of micro-RNA in women with different ovarian reserve

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Masood Abu-Halima ◽  
Lea Simone Becker ◽  
Basim M. Ayesh ◽  
Simona Lucia Baus ◽  
Amer Hamza ◽  
...  
Keyword(s):  
Ovarian Reserve ◽  
Independent Set ◽  
Micro Rna ◽  
Mirna Array ◽  
Reverse Transcription Quantitative Pcr ◽  
Diagnostic Approaches ◽  
Auc Value ◽  
Mirna Abundance

AbstractWomen undergoing infertility treatment are routinely subjected to one or more tests of ovarian reserve. Therefore, an adequate assessment of the ovarian reserve is necessary for the treatment. In this study, we aimed to characterize the potential role of microRNAs (miRNAs) as biomarkers for women with different ovarian reserves. A total of 159 women were recruited in the study and classified according to their anti-Müllerian hormone (AMH) level into three groups: (1) low ovarian reserve (LAMH, n = 39), (2) normal ovarian reserve (NAMH, n = 80), and (3) high ovarian reserve (HAMH, n = 40). SurePrint Human miRNA array screening and reverse transcription-quantitative PCR (RT-qPCR) were respectively employed to screen and validate the miRNA abundance level in the three tested groups. Compared with NAMH, the abundance level of 34 and 98 miRNAs was found to be significantly altered in LAMH and HAMH, respectively. The abundance level of miRNAs was further validated by RT-qPCR in both, the screening samples as well as in an independent set of validation samples. The abundance levels of the validated miRNAs were significantly correlated with the AMH level. The best AUC value for the prediction of the increase and decrease in the AMH level was obtained for the miR-100-5p and miR-21-5p, respectively. The level of miRNAs abundance correlates with the level of AMH, which may serve as a tool for identifying women with a different ovarian reserve and may help to lay the ground for the development of novel diagnostic approaches.

Abstract 644: Role of Micro RNA LET-7F in Cigarette Smoke-Induced Impairment of Neovascularization

2015 ◽  
Vol 35 (suppl_1) ◽  
Author(s):  
Wahiba Dhahri ◽  
Sylvie Dussault ◽  
Paola Haddad ◽  
Julie Turgeon ◽  
Sophie Tremblay ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Vascular Endothelial Cells ◽  
Capillary Density ◽  
Tube Formation ◽  
Micro Rna ◽  
Cellular Migration ◽  
Mirna Array ◽  
Inhibition Of Angiogenesis

Background: Exposure to cigarette smoke is associated with impaired neovascularization in response to ischemia. The precise mechanisms involved in that process remain to be determined. Micro RNA (miR) are emerging as key regulators of several physiological processes, including angiogenesis. Here we investigated the potential role of miRs for the modulation of neovascularization in the context of cigarette smoking. Methods and Results: Human Umbilical Vascular Endothelial Cells (HUVECs) were exposed or not to cigarette smoke extracts (CSE). Using Affimetrix GeneChip miRNA array analysis, we found that the pro-angiogenic miR let-7f was downregulated by 40% in HUVECs exposed to CSE. Using an inhibitor of let-7f, we demonstrated reduced migration and tube formation in HUVECs, reproducing the phenotype induced by CSE. A let-7f mimic could rescue cellular migration and tube formation in HUVECs exposed to CSE. Moreover, the expression of let-7f is significantly reduced in the ischemic muscles of mice exposed to cigarette smoke (CS). In vivo, hindlimb ischemia was surgically provoked by femoral artery removal to mice exposed (SMK) or not to CS for two weeks with a local injection of a control or a let-7f mimic. Let-7f mimic could rescue blood flow recuperation and capillary density in ischemic muscles 21 days post-ischemia associated with improved mobility. We found that CS was associated with reduced number of endothelial progenitor cells (EPCs) and impairment of angiogenic activities. Importantly, let-7f mimic rescued EPC number and EPC functional activities in SMK group. TGF-β-RI and HIF1AN are predicted to be targeted by let-7f and both are increased in SMK mice, whereas the expression of HIF-1a and VEGF are reduced in these mice. Interestingly, SMK mice injected with a let-7f mimic have decreased muscle expression of TGF-β-RI and HIF1AN associated with normalized HIF-1 and VEGF expression. Conclusion: Our results suggest that a reduction in the expression of let-7f could be involved in the cigarette smoke-induced inhibition of angiogenesis through modulation of TGF-β-RI and HIF1AN. Overexpression of let-7f using a miR mimic could constitute a novel therapeutic strategy to improve ischemia-induced neovascularization in pathological conditions.

At the heart of programming: the role of micro-RNAs

2018 ◽  
Vol 9 (6) ◽  
pp. 615-631 ◽  
Author(s):  
B. Siddeek ◽  
C. Mauduit ◽  
C. Yzydorczyk ◽  
M. Benahmed ◽  
U. Simeoni
Keyword(s):  
Heart Development ◽  
Disease Risk ◽  
Heart Diseases ◽  
Underlying Mechanism ◽  
Micro Rna ◽  
Environmental Challenges ◽  
Micro Rnas ◽  
Heart Functions ◽  
Heart Disease Risk

AbstractEpidemiological and experimental observations tend to prove that environment, lifestyle or nutritional challenges influence heart functions together with genetic factors. Furthermore, when occurring during sensitive windows of heart development, these environmental challenges can induce an ‘altered programming’ of heart development and shape the future heart disease risk. In the etiology of heart diseases driven by environmental challenges, epigenetics has been highlighted as an underlying mechanism, constituting a bridge between environment and heart health. In particular, micro-RNAs which are involved in each step of heart development and functions seem to play a crucial role in the unfavorable programming of heart diseases. This review describes the latest advances in micro-RNA research in heart diseases driven by early exposure to challenges and discusses the use of micro-RNAs as potential targets in the reversal of the pathophysiology.

Role of circulating micro-RNA-21 as a noninvasive biomarker for detection of colorectal cancer

2021 ◽  
Vol 34 (1) ◽  
pp. 124
Author(s):  
MohamedH Gohar ◽  
EhabA Abdel-Atti ◽  
El-SayedI El-Shaib ◽  
MohamedA Shehata ◽  
AhmedA Sonbl ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Micro Rna ◽  
Noninvasive Biomarker

Deregulation of miR-21 and miR-29a in Cervical Cancer Related to HPV Infection

MicroRNA ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 110-115 ◽  
Author(s):  
Sara Zamani ◽  
Amir Sohrabi ◽  
Seyed Masoud Hosseini ◽  
Marjan Rahnamaye-Farzami ◽  
Abolfazl Akbari
Keyword(s):  
Cervical Cancer ◽  
Cancer Progression ◽  
Expression Patterns ◽  
Invasive Cervical Cancer ◽  
Hpv Infection ◽  
Micro Rna ◽  
Internal Reference ◽  
Specific Primers ◽  
Papilloma Virus

Background:Early diagnosis is an important factor to improve the survival of Invasive Cervical Cancer (ICC) patients. Molecular biomarkers such as micro RNA (miRNA) can be used in the early detection of ICC. The expression of miR-21 and miR-29a are deregulated in many types of human cancers.Objective:The aim of this study was to investigate the differences in miR-21 and miR-29a expression patterns in the Human Papilloma Virus (HPV) infection and various grades of cervical cancer among Iranian women.Methods:Small RNAs were extracted from positive for HPV, cervical cancer and healthy samples from 43, 50 and 46 individuals, respectively. Expression levels of miR-21 and miR-29a were analyzed by SYBR Green real-time RT-PCR using specific primers, and 5s rRNA as the internal reference gene.Results:Results have shown a significant increase in miR-21 and decrease in miR-29 in cancerous samples in comparison with the control groups (P < 0.0001).Conclusion:This study illustrated that miR-21 and miR-29a could be operated as an oncogene and tumor-suppressor in cervical cancer progression. More studies are needed to demonstrate the role of miR-21 and miR-29a as potential biomarkers for the diagnosis of cervical cancer in future investigations.

scholarly journals Maternal Recognition of Pregnancy in the Horse: Are MicroRNAs the Secret Messengers?

2020 ◽  
Vol 21 (2) ◽  
pp. 419 ◽  
Author(s):  
Katrien Smits ◽  
Yannick Gansemans ◽  
Laurentijn Tilleman ◽  
Filip Van Nieuwerburgh ◽  
Margot Van De Velde ◽  
...  
Keyword(s):  
Differential Expression ◽  
Micro Rna ◽  
Steroid Synthesis ◽  
Tissue Samples ◽  
Strong Basis ◽  
Embryonic Tissues ◽  
Uterine Fluid ◽  
Maternal Recognition Of Pregnancy ◽  
And Control

The signal for maternal recognition of pregnancy (MRP) has still not been identified in the horse. High-throughput molecular biology at the embryo–maternal interface has substantially contributed to the knowledge on pathways affected during MRP, but an integrated study in which proteomics, transcriptomics and miRNA expression can be linked directly is currently lacking. The aim of this study was to provide such analysis. Endometrial biopsies, uterine fluid, embryonic tissues, and yolk sac fluid were collected 13 days after ovulation during pregnant and control cycles from the same mares. Micro-RNA-Sequencing was performed on all collected samples, mRNA-Sequencing on the same tissue samples and mass spectrometry was conducted previously on the same fluid samples. Differential expression of miRNA, mRNA and proteins showed high conformity with literature and confirmed involvement in pregnancy establishment, embryo quality, steroid synthesis and prostaglandin regulation, but the link between differential miRNAs and their targets was limited and did not indicate the identity of an unequivocal signal for MRP in the horse. Differential expression at the embryo–maternal interface was prominent, highlighting a potential role of miRNAs in embryo–maternal communication during early pregnancy in the horse. These data provide a strong basis for future targeted studies.

P2581Unique molecular profile of exosomes from epicardial fat of patients with atrial fibrillation

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
O Shaihov - Teper ◽  
E Ram ◽  
L Levin - Kotler ◽  
N Naftali - Shani ◽  
J Leor
Keyword(s):  
Heart Surgery ◽  
Micro Rna ◽  
Epicardial Fat ◽  
Molecular Profile ◽  
Atrial Remodeling ◽  
Intercellular Signaling ◽  
Intracellular Compartments ◽  
Novel Biomarkers ◽  
First Time

Abstract Background and aim Exosome, the smallest vesicles that originate from intracellular compartments, contains and delivers specific bioactive proteins, lipids, mRNA, and miRNA to facilitate intercellular signaling. However, the role and mechanism of exosomes in atrial remodeling and fibrillation (AF) has not been determined. While epicardial fat (eFat) and inflammation have been linked to atrial remodeling and fibrillation (AF), the role of eFat exosomes in the pathogenesis of AF remains unknown. Thus, we aimed to determine whether eFat exosomes play a role in the initiation and progression of AF. Methods and results We collected eFat specimens from patients with and without chronic or paroxysmal AF undergoing heart surgery. Isolated fat specimens were cut into small pieces and incubated as organ cultures. We isolated exosomes from the medium of the explant by differential ultra-centrifugation and characterized the vesicle size distributions, morphology, specific markers, and molecular cargo. Immunoblotting of CD63, CD81 and TSG101 confirmed the presence of exosomes (Fig.1 B). Significantly, eFat from patients with AF secreted higher amounts of exosomes compared with controls (p<0.001), which confirmed by Nanoparticle Tracking Analysis (NTA) (Fig.1 A). The levels of exosomal pro-inflammatory and pro-fibrotic cytokines were higher in exosomes from patients with, compared with those without, AF (Fig.1 C, D). Measurements of micro RNA (miRNA) by real-time PCR showed lower levels of anti-fibrotic miRNA-133a (p=0.2), and higher levels of pro-fibrotic miRNA-146b (p=0.2) in exosomes from patients with, compared with those without, AF (Fig.1 E, F) Conclusion We show, for the first time, that exosomes from eFat of patients with AF have a unique pro-inflammatory and pro-fibrotic profile. We suggest a novel mechanism that links eFat to AF and eFat exosomes as novel biomarkers and potential therapeutic targets for AF.

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