scholarly journals Acetylshikonin suppressed growth of colorectal tumour tissue and cells by inhibiting the intracellular kinase, T‐lymphokine‐activated killer cell‐originated protein kinase.

2020 ◽  
Vol 177 (10) ◽  
pp. 2303-2319
Author(s):  
Ran Zhao ◽  
Bu Young Choi ◽  
Lixiao Wei ◽  
Mangaladoss Fredimoses ◽  
Fanxiang Yin ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Killer Cell ◽  
Tumour Tissue ◽  
Colorectal Tumour ◽  
Lymphokine Activated Killer Cell

scholarly journals 3-Deoxysappanchalcone Inhibits Skin Cancer Proliferation by Regulating T-Lymphokine-Activated Killer Cell-Originated Protein Kinase in vitro and in vivo

2021 ◽  
Vol 9 ◽  
Author(s):  
Xiaorong Fu ◽  
Ran Zhao ◽  
Goo Yoon ◽  
Jung-Hyun Shim ◽  
Bu Young Choi ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Protein Kinase ◽  
Skin Cancer ◽  
Cell Growth ◽  
Mouse Model ◽  
Killer Cell ◽  
Skin Cancer Prevention ◽  
Lymphokine Activated Killer Cell

BackgroundSkin cancer is one of the most commonly diagnosed cancers worldwide. The 5-year survival rate of the most aggressive late-stage skin cancer ranges between 20 and 30%. Thus, the discovery and investigation of novel target therapeutic agents that can effectively treat skin cancer is of the utmost importance. The T-lymphokine-activated killer cell-originated protein kinase (TOPK), which belongs to the serine-threonine kinase class of the mitogen-activated protein kinase kinase (MAPKK) family, is highly expressed and activated in skin cancer. The present study investigates the role of 3-deoxysappanchalcone (3-DSC), a plant-derived functional TOPK inhibitor, in suppressing skin cancer cell growth.PurposeIn the context of skin cancer prevention and therapy, we clarify the effect and mechanism of 3-DSC on different types of skin cancer and solar-simulated light (SSL)-induced skin hyperplasia.MethodsIn an in vitro study, western blotting and in vitro kinase assays were utilized to determine the protein expression of TOPK and its activity, respectively. Pull-down assay with 3-DSC and TOPK (wild-type and T42A/N172 mutation) was performed to confirm the direct interaction between T42A/N172 amino acid sites of TOPK and 3-DSC. Cell proliferation and anchorage-independent cell growth assays were utilized to determine the effect of 3-DSC on cell growth. In an in vivo study, the thickness of skin and tumor size were measured in the acute SSL-induced inflammation mouse model or SK-MEL-2 cell-derived xenografts mouse model treated with 3-DSC. Immunohistochemistry analysis of tumors isolated from SK-MEL-2 cell-derived xenografts was performed to determine whether cell-based results observed upon 3-DSC treatment could be recapitulated in vivo.Results3-DSC is able to inhibit cell proliferation in skin cancer cells in an anchorage-dependent and anchorage-independent manner by regulation of TOPK and its related signaling pathway in vitro. We also found that application of 3-DSC reduced acute SSL-induced murine skin hyperplasia. Additionally, we observed that 3-DSC decreased SK-MEL-2 cell-derived xenograft tumor growth through attenuating phosphorylation of TOPK and its downstream effectors including ERK, RSK, and c-Jun.ConclusionsOur results suggest that 3-DSC may function in a chemopreventive and chemotherapeutic capacity by protecting against UV-induced skin hyperplasia and inhibiting tumor cell growth by attenuating TOPK signaling, respectively.

Inhibition of lymphokine-activated killer cell generation by blocking factors in sera of patients with head and neck cancer

1990 ◽  
Vol 31 (3) ◽  
pp. 176-181 ◽  
Author(s):  
Samuel P. Bugis ◽  
Eva Lotzová ◽  
Howard E. Savage ◽  
Jeane P. Hester ◽  
Tamas Racz ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer ◽  
Killer Cell ◽  
Cell Generation ◽  
Lymphokine Activated Killer Cell ◽  
Blocking Factors
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